Study of radiation-induced cluster DNA damage- its structure and repair susceptibility

2013 Fiscal Year Final Research Report

• Project/Area Number: 21310041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: YOKOYA Akinari 独立行政法人日本原子力研究開発機構, 原子力科学研究部門先端基礎研究センター, 研究主席 (10354987)
• Co-Investigator(Kenkyū-buntansha): SHIKAZONO Naoya 独立行政法人日本原子力研究開発機構・ 原子力科学研究部門, 量子ビーム応用研究センタ ー, 研究主幹 (10354961) ; WATANABE Ritsuko 独立行政法人日本原子力研究開発機構・ 原子力科学研究部門, 原子力基礎工学研究センタ ー, 研究副主幹 (10360439) ; FUJII Kentaro 独立行政法人日本原子力研究開発機構・ 原子力科学研究部門, 先端基礎研究センター, 研究副主幹 (00360404) ; NOGUCHI Miho 独立行政法人日本原子力研究開発機構・ 原子力科学研究部門, 先端基礎研究セ ンター, 研究員 (40455283) ; HIGUCHI Mariko 独立行政法人日本原子力研究開発機構・ 原 子力科学研究部門, 原子力基礎工学研究 センタ ー, 任期付研究員 (90370460) ; OKA Toshitaka 東北大学, 高等教育開発推進センタ ー, 助教 (70339745)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Keywords: クラスターDNA損傷 / 放射線 / シンクロトロン放射光 / イオンビーム / DNA修復 / 軟X線 / EPR / DNAラジカル

Research Abstract

In order to clarify the induction processes of clustered DNA damage, we have investigated the radiation effect on DNA molecules using both biochemical and spectroscopic techniques. We determined the yields of clustered DNA damage induced by various ion beam exposure. The data were analyzed using a computer simulation method to establish a damage induction model. Further we found a novel radical process involved in the DNA damage induction using an EPR apparatus installed in a high brilliance synchrotron radiation facility. These results have been presented in a number of symposiums or workshops, and also published as 25 peer review papers.

Research Products

• [Journal Article] A. Induction of DNA damage, including abasic sites, in plasmid DNA by carbon ion and X-ray irradiation (2013)
  • Author(s): Shiina, T., Watanabe, R., Shiraishi, I., Suzuki, M., Sugaya, Y., Fujii, K. and Yokoya
  • Journal Title: Radiat. Environ. Biophys. Volume: 52 Pages: 99-112
  • Peer Reviewed
• [Journal Article] M. Unpaired electron species in calf thymus DNA thin films induced by nitrogen and oxygen K-shell photoabsorption (2012)
  • Author(s): Oka, T., Yokoya, A., Fujii, K., Fukuda, Y. and Ukai
  • Journal Title: Phys. Rev. Lett. Volume: 109
  • Peer Reviewed
• [Journal Article] The mutagenic potential of 8-oxoG/single strand break-containing clusters depends on their relative positions (2012)
  • Author(s): Noguchi, M., Urushibara, A., Yokoya
  • Journal Title: Mutat. Res. Volume: 732 Pages: 34-42
  • Peer Reviewed
• [Journal Article] A. A novel technique using DNA denaturation to detect multiply induced single-strand breaks in a hydrated plasmid DNA molecule by X-ray and 4He2+ ion irradiation (2011)
  • Author(s): Yokoya, A., Shikazono, N., Fujii, K., Noguchi, M. and Urushibara
  • Journal Title: Radiat. Protect. Dosim Volume: 143 Pages: 219-225
  • Peer Reviewed
• [Journal Article] A. The yield, processing, and biological consequences of clustered DNA (2009)
  • Author(s): Shikazono N., Noguchi, M., Fujii, K., Urushibara, A. and Yokoya
  • Journal Title: J. Radiat. Res Volume: (Invited Review) Pages: 27-36
  • Peer Reviewed
• [Journal Article] A. Nucleobase Lesions and Strand Breaks in Dry DNA Thin Film selectively induced by Monochromatic Soft X-rays (2009)
  • Author(s): Fujii, K., Shikazono, N. and Yokoya
  • Journal Title: J. Phys. Chem. B Volume: 113 Pages: 16007-16015
  • Peer Reviewed
• [Presentation] 高輝度軟X線を用いた不対電子観測によるDNA損傷の物理学的プロセス (2013)
  • Author(s): 岡壽崇
  • Organizer: 物構研サイエンスフェスタ2013
  • Place of Presentation: つく ば市
  • Year and Date: 20130318-19
  • Invited
• [Presentation] Molecular and Cellular Effect of Ionizing Radiation (2013)
  • Author(s): Yokoya, A. , Oka, T. , Fujii, K. , Shiina, T. , Sugaya, Y. , Shiraishi, I. , Urushibara, A. , Narita1, A. , Noguchi, M. and Watanabe, R.
  • Organizer: The 16th SANKEN International The 11th SANKEN Nanotechnology Symposium
  • Place of Presentation: saka, Japan
  • Year and Date: 20130122-23
  • Invited
• [Presentation] DNA damage by soft X-ray exposure at oxygen K-edge (2013)
  • Author(s): Sugaya, Y. , Nartita, A. , Fujii, K. and Yokoya, A.
  • Organizer: 1st Conference on Light and Particle Beams in Materials Science
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 20130000
• [Presentation] Dependence of the yields of AP sites and AP clusters produced in plasmid DNA on scavenging capacity and LET (2012)
  • Author(s): Shiina, T. , Sugaya, Y. , Shiraishi, I. , Watanabe, R. , Suzuki, M. , Yokoya, A.
  • Organizer: The 12th International Workshop on Radiation Damage to DNA
  • Place of Presentation: Prague, Czech Republic
  • Year and Date: 20120902-06
• [Presentation] 放射線によるゲノムDNA損傷の初期過程と生体修復 (2012)
  • Author(s): 横谷明徳
  • Organizer: 日本物理学会第67回年次大会
  • Place of Presentation: 西宮市
  • Year and Date: 20120324-27
  • Invited
• [Presentation] Study of Unpaired Electron Species in DNA Film Induced by Nitrogen and Oxygen K-shell Photoabsorption (2011)
  • Author(s): Oka, T.
  • Organizer: The 7th Auger Symposium
  • Place of Presentation: Julich, Germany
  • Year and Date: 20110824-26
• [Presentation] Various modes of DNA damage formation by the direct effect of radiation (2010)
  • Author(s): Yokoya, A.
  • Organizer: The 56th Annual Meeting of Radiation Research Society
  • Place of Presentation: Hawaii, USA
  • Year and Date: 20100925-29
  • Invited
• [Book] Spectroscopic study of radiation-induced DNA lesions and their susceptibility to enzymatic repair. In: Charged particle and photon interactions with matter-recent advances, applications and interfaces(Eds. , Y. Hatano, Y. Katsumura, and A. Mozumder) (2011)
  • Author(s): Yokoya, A. , Fujii, K. , Shikazono, N. and Ukai
  • Total Pages: 543-574
  • Publisher: CRC/Taylor & Francis Group
• [Remarks]
  • URL: http://asrc.jaea.go.jp/soshiki/gr/yokoya-gr/