2011 Fiscal Year Final Research Report
Realization of a secure and safe proteinaceous-insecticide based on the directed evolution of the insecticidal toxin from soil bacterium, Bacillus thuringiensis
| Project/Area Number |
21310051
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
SATO Ryoichi 東京農工大学, 大学院・農学研究院, 教授 (30235428)
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| Co-Investigator(Kenkyū-buntansha) |
KUNIMI Yasuhisa 東京農工大学, 大学院・農学研究院, 教授 (50195476)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 環境負荷低減技術 / 殺虫剤 / Cry toxin / 進化分子工学 / Bacillus thuiringiensis / タンパク質殺虫剤 / カドヘリン様受容体 |
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| Research Abstract |
It was indicated that Cry toxin mutants affinity-maturated to cadherin-like receptor is expected to be easily acquired, if mutation is introduced in loop 2 or loop 3 of Cry toxin, mutant Cry toxins are displayed on the T7 phage, and mutant-toxin-displaying phages are screened by bio-panning using cadherin-like receptor. That is to say, base technology was developed for the directed evolution of Cry toxin by which any available Cry toxins are possible to be changed to be highly active to any insect pests
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