2011 Fiscal Year Final Research Report
Development of oligonucleotide-based artificial transcription factor based on the higher-order structure of nucleic acids
| Project/Area Number |
21350094
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
OBIKA Satoshi 大阪大学, 大学院・薬学研究科, 教授 (80243252)
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| Co-Investigator(Kenkyū-buntansha) |
KODAMA Tetsuya 大阪大学, 大学院・薬学研究科, 助教 (00432443)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 核酸 / 人工核酸 / 三重鎖 / 4重鎖 / 分子認識 / 転写制御 |
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| Research Abstract |
An artificial nucleic acid bearing a switching function controlling structural properties, a useful nucleic acid unit for conjugation chemistry, and a couple of bridged nucleic acid guanine analogs were synthesized. Non-natural oligonucleotides that can form cytosine quadruplex structure at physiological conditions were synthesized as well. Moreover, invasive technique for evaluating gene expression change was established, and it was shown that artificial oligonucleotide could induce a change in gene expression pattern. These results indicate that our developing artificial nucleic acids are effective to gene expression regulation.
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