2011 Fiscal Year Final Research Report
Development of a pyrosequence-based technique for HIV drug-resistance diagnosis
| Project/Area Number |
21360408
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Waseda University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUGIURA Wataru (独)国立病院機構名古屋医療センター, 臨床研究センター感染免疫研究部, 部長 (70300936)
OKAMURA Yoshiko 広島大学, 先端物質科学研究科, 准教授 (80405513)
MORI Tetsushi 早稲田大学, 理工学術院, 助教 (00590100)
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| Project Period (FY) |
2009 – 2011
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| Keywords | pyrosequencing / HIV / quasispecies / 同時並列個別増幅 / 一分子計測 |
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| Research Abstract |
The aim of this research is to develop a single-cell analytical technique based on pyrosequencing to accurately identify diverse mutations within HIV and to conduct sequence-based elucidation of the mechanisms related to HIV quasispecies formation. As a result, we were successful in the establishment of a system for measuring cDNA molecules to as low as 10 copies. Furthermore, we were also successful in tracing the coevolution between the subtypes of HIV quasispecies-related proviral DNA, Gag and PR.
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