Structural analysis of A1 protein-telomere-telomerase complex, and development of inhibition of telomerase

2011 Fiscal Year Final Research Report

• Project/Area Number: 21370047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University (2010-2011); Yokohama City University (2009)
• Principal Investigator: KATAHIRA Masato 京都大学, エネルギー理工学研究所, 教授 (70211844)
• Co-Investigator(Kenkyū-buntansha): NAGATA Takashi 京都大学, エネルギー理工学研究所, 助教 (10415250)
• Project Period (FY): 2009 – 2011
• Keywords: テロメア / テロメラーゼ / A1タンパク質 / 癌 / デコイ

Research Abstract

We have determined the structure of the Musashi-target RNA binary complex with the assist of long-range structural information. With the same assist, the structure of the A1 protein-telomere DNA-telomerase RNA ternary complex has been analyzed. It has been shown that A1 works as a bridge between telomere DNA and telomerase RNA. This suggests that A1 functions to bring telomerase to telomere DNA. The strategy to inhibit telomerase with decoy nucleic acids has also been suggested to be promising.

Research Products

• [Journal Article] Structure of Musashi1 in a complex with target RNA : The role of aromatic stacking interactions (2012)
  • Author(s): Ohyama, T., Nagata, T., Tsuda, K., Kobayashi, N., Imai, T., Okano, H., Yamazaki, T. and Katahira, M
  • Journal Title: Nucleic Acids Res Volume: 10 Pages: 3218-3231
  • DOI: doi:10.1093/nar/gkr1139
  • Peer Reviewed
• [Journal Article] Intelligent ribozyme that switches its activity in response to K+ throughquadruplex formation (2012)
  • Author(s): Nagata, T., Sakurai, Y., Hara, Y., Mashima, T. and Katahira, M.
  • Journal Title: FEBS J. Volume: 279 Pages: 1456-1463
  • DOI: doi:10.1111/j.1742-4658.2012.08538.x
  • Peer Reviewed
• [Journal Article] Presence of alternating glucosaminoglucan in the sheath of Thiothrix nivea (2012)
  • Author(s): Takeda, M., Kondo, K., Ymamada, M., Sumikawa, M., Koizumi, J., Mashima, T. and Katahira, M.
  • Journal Title: Internet J. Biol. Volume: 50 Pages: 236-244
  • DOI: doi:10.1016/j.ijbiomac.2011.10.024
  • Peer Reviewed
• [Journal Article] Structural aspects for the recognition of ATPby ribonucleopeptide receptors (2011)
  • Author(s): Nakano, S., Mashima, T., Matsugami, A., Inoue, M., Katahira, M. and Morii, T.
  • Journal Title: J. Am. Chem. Soc. Volume: 133 Pages: 4567-4579
  • DOI: DOI:10.1021/ja110725d
  • Peer Reviewed
• [Journal Article] Heat shock protein 70 inhibitsHIV-1 Vif-mediated ubiquitination and degradation of APOBEC3G (2011)
  • Author(s): Sugiyama, R., Nishitsuji, H., Furukawa, A., Katahira, M., Habu, Y., Takeuchi, H., Ryo, A. and Takaku, H.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 10051-10057
  • DOI: doi:10.1074/jbc.M110.166108
  • Peer Reviewed
• [Journal Article] Study of a novel glycoconjugate, thiopeptidoglycan, and a novel polysaccharide lyase (2011)
  • Author(s): Kondo, K., Takeda, M., Ejima, W., Kawasaki, Y., Umezu, T., Yamada, M., Koizumi, J., Mashima, T., and Katahira, M.
  • Journal Title: Internat. J. Biol. Macro mol. Volume: 48 Pages: 256-262
  • DOI: doi:10.1016/j.ijbiomac.2010.11.009
  • Peer Reviewed
• [Journal Article] Solution structures of the trihelix DNA-binding domains of the wild-type and a phosphomimetic mutant of Arabidopsis GT-1 (2010)
  • Author(s): Nagata, T., Niyada, E., Fujimoto, N., Nagasaki, Y., Noto, K., Miyanoiri, Y., Murata, J., Hiratsuka, K. and Katahira, M.
  • Journal Title: Proteins Volume: 78 Pages: 3033-3047
  • DOI: DOI:10.1002/prot.22827
  • Peer Reviewed
• [Journal Article] Solubilization and structural determination of a glycoconjugate which isassembled into the sheath of Leptothrixcholodnii (2010)
  • Author(s): Takeda, M., Kondo, K., Yamada, M., Koizumi, J., Mashima, T., Matsugami, A. and Katahira, M.
  • Journal Title: Int. J. Biol. Macro mol Volume: 46 Pages: 206-211
  • DOI: doi:10.1016/j.ijbiomac.2009.12.006
  • Peer Reviewed
• [Journal Article] Structure, interaction, and real-time monitoring of the enzymatic reaction of wild-type APOBEC3G (2009)
  • Author(s): Furukawa, A., Nagata, T., Matsugami, A., Habu, Y., Sugiyama, R., Hayashi, F., Yokoyama, S., Takaku, H. and Katahira, M.
  • Journal Title: EMBO J. Volume: 28 Pages: 440-451
  • DOI: doi:10.1038/emboj.2008.290
  • Peer Reviewed
• [Journal Article] Uniquequadruplex structure and interaction of anRNA aptamer against bovine prion protein (2009)
  • Author(s): Mashima, T., Matsugami, A., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Journal Title: Nucleic Acids Res. Volume: 37 Pages: 6249-6258
  • DOI: doi:10.1093/nar/gkp647
  • Peer Reviewed
• [Journal Article] The orientation of the ends of G-quadruplex structures investigated using end-extended oligonucleotides (2009)
  • Author(s): Sannohe, Y., Sato, K., Matsugami, A., Shinohara, K., Mashimo, T., Katahira, M. and Sugiyama, H.
  • Journal Title: Bioorg. Medici. Chem. Volume: 17 Pages: 1870-1875
  • DOI: doi:10.1016/j.bmc.2009.01.051
  • Peer Reviewed
• [Journal Article] Structural studies of an RNA aptamer containing GGArepeats under ionic conditions using microchip electrophoresis (2009)
  • Author(s): Nishikawa, F., Murakami, K., Matsugami, A., Katahira, M. and Nishikawa, S.
  • Journal Title: Oligonucleotides Volume: 19 Pages: 179-190
  • DOI: doi:10.1089/oli.2008.0167
  • Peer Reviewed
• [Presentation] Sliding-direction-dependent activity of anti-HIV enzyme, and structural basis of anti-prion activity of RNA aptamer (2012)
  • Author(s): Furukawa, A., Mashima, T., Sugase, K., Fujiwara, H., Nagata, T., Morishita, R., Ryo, A., Nishikawa, F., Nishikawa, S., Kamatari, Y., Kuwata, K. and Katahira, M.
  • Organizer: Korea-Japan Bilateral NMR Symposium
  • Place of Presentation: Sapporo
  • Year and Date: 2012-03-16
• [Presentation] Towards evaluation of wood cell wall structures and those biodegradation using solution NMR (2012)
  • Author(s): Nishimura, H., Kozawa, Y., Okamura, H., Watanabe, T. and Katahira, M.
  • Organizer: Korea-Japan Bilateral NMR Symposium
  • Place of Presentation: Sapporo
  • Year and Date: 2012-03-16
• [Presentation] Recognition of prion protein by the RNA aptamer (2012)
  • Author(s): Mashima, T., Fujiwara, H., Nishikawa, F., Saimura, M., Kamatari, Y., Kuwata, K., Imamura, M., Yokoyama, T., Nishikawa, S. and Katahira, M.
  • Organizer: Korea-Japan Bilateral NMR Symposium
  • Place of Presentation: Sapporo
  • Year and Date: 2012-03-16
• [Presentation] Base-deamination rate dependent on the direction of sliding of an enzyme along DNA as revealed by numerical analysis of NMR data, and wood biomass studied by solution NMR (2011)
  • Author(s): Nishimura, H., Furukawa, A., Okamura, H., Kozawa, Y., Morishita, R., Ryo, A., Sugase, K., Watanabe, T. and Katahira, M.
  • Organizer: The International Symposium on Nuclear Magnetic Resonance 2011
  • Place of Presentation: Yokohama
  • Year and Date: 20111115-18
• [Presentation] r(GGA) 4 chimeric ribozyme switches its activity in response to K+ via quadruplex formation (2011)
  • Author(s): Nagata, T., Sakurai, Y., Mashima, T. and Katahira, M.
  • Organizer: The 38th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Sapporo
  • Year and Date: 20111109-11
• [Presentation] Elucidation of functions on APOBEC3DE and APOBEC3F based on structure-oriented analysis (2011)
  • Author(s): Iwaoka, R., Furukawa, A., Okamura, H. and Katahira, M.
  • Organizer: 3^<rd> G-COE International Symposium Zero Carbon Energy 2011
  • Place of Presentation: 水原
  • Year and Date: 20110818-19
• [Presentation] Structural basis of the high affinity of the RNA aptamer against prion protein (2011)
  • Author(s): Mashima, T., Fujiwara, H., Koshida, T., Saimura, M., Imamura, M., Yokoyama, T., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: The 16th annual meeting of the RNA society
  • Place of Presentation: Kyoto
  • Year and Date: 20110614-18
• [Presentation] Structure and interaction of an RNA aptamer with potential therapeutic use (2010)
  • Author(s): Mashima, T., Matsugami, A., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: The International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii
  • Year and Date: 20101215-20
• [Presentation] Unique quadruplex structure and interaction of r(GGAGGAGGAGGA) with protein (2010)
  • Author(s): Mashima, T., Matsugami, A., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: The International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii
  • Year and Date: 20101215-20
• [Presentation] Structural aspects of the fluorescence intensity changes of the ATP-binding ribonucleopeptide sensor (2010)
  • Author(s): Yoshioka, K., Daidai, M., Katahira, M. and Watanabe, T.
  • Organizer: The International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii
  • Year and Date: 20101215-20
• [Presentation] Structure and interaction of RNA aptamer againstprion protein in complex with the partial binding peptide (2010)
  • Author(s): Mashima, T., Koshida, T., Saimura, M., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: The 37th International Symposium on Nucleic Acids Chemistry 2010
  • Place of Presentation: Yokohama
  • Year and Date: 20101110-12
• [Presentation] Structure and interaction of RNA aptamer against prion protein in complex with the partial binding peptide (2010)
  • Author(s): Nagata, T., Niyada, E., Fujimoto, N., Nagasaki, Y., Noto, K., Miyanoiri, Y., Murata, J., Hiratsuka, K. and Katahira, M.
  • Organizer: The 37th International Symposium on Nucleic Acids Chemistry 2010
  • Place of Presentation: Yokohama
  • Year and Date: 20101110-12
• [Presentation] A mechanism for a increase in DNA-binding affinity of the trihelix DNA-binding domain via its phosphrylation (2010)
  • Author(s): Nakano, S., Mashima, T., Tainaka, K., Katahira, M. and Morii, T.
  • Organizer: The 37th International Symposium on Nucleic Acids Chemistry 2010
  • Place of Presentation: Yokohama
  • Year and Date: 20101110-12
• [Presentation] Real-time monitoring of the enzymatic reaction of APOBEC3G possessing anti-HIV activity, and the structure and interaction of the RNA aptamer against prion protein (2010)
  • Author(s): Furukawa, A., Mashima, T., Nagata, T., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: The 10th KIAS Conference on Protein Structure and Function
  • Place of Presentation: Seoul
  • Year and Date: 20100930-1002
• [Presentation] Real-time monitoring of the enzymatic reaction of APOBEC3G possessing anti-HIV activity, and the structure of the RNA aptamer against prion protein (2010)
  • Author(s): Mashima, T., Furukawa, A., Nagata, T., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: XXIV International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Cairns
  • Year and Date: 20100822-27
• [Presentation] The real-time monitoring of the enzymatic reaction of an anti-HIV factor APOBEC3G (2010)
  • Author(s): Furukawa, A., Nagata, T., Morishita, R., Iwaoka, R. and Katahira, M.
  • Organizer: XXIV International Conference on Magnetic Resonance in Biological Systems
  • Place of Presentation: Cairns
  • Year and Date: 20100822-27
• [Presentation] The analysis of the mechanism of an enzyme reaction toward the development of new energy systems (2010)
  • Author(s): Iwaoka, R., Furukawa, A. and Katahira, M.
  • Organizer: The 2nd International Symposium of Kyoto University Global COE Program Zero Carbon Energy Kyoto 2010
  • Place of Presentation: Kyoto
  • Year and Date: 20100819-20
• [Presentation] Real-time monitoring of the cytidine deamination along single-stranded DNA by an anti-HIV factor (2010)
  • Author(s): Furukawa, A., Mashima, T., Matsugami, A., Nagata. T., Sugiyama, R., Takaku, H., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: The International Symposium on Nuclear Magnetic Resonance 2011
  • Place of Presentation: Seoul
  • Year and Date: 20100517-19
• [Presentation] The real-time monitoring of the enzymatic reaction of an anti-HIV factor APOBEC3G (2010)
  • Author(s): Furukawa, A., Nagata, T., Morishita, R., Iwaoka, R. and Katahira, M.
  • Organizer: Korea-Japan Bilateral NMR Symposium
  • Year and Date: 20100000
• [Presentation] Real-time monitoring of the enzymatic reaction of APOPBEC3G possessing anti-HIV activity, and the structure of the RNA aptamer against prion (2009)
  • Author(s): Furukawa, A., Mashima, T., Nagata, T., Takaku, H., Matsugami, A., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: The 3rd Asia-Pacific NMR Symposium
  • Place of Presentation: Cheju
  • Year and Date: 20091115-18
• [Presentation] Structural analysis of ribonucleopeptide aptamer against ATP (2009)
  • Author(s): Mashima, T., Matsugami, A., Nakano, S., Inoue, M., Fukuda, M., Morii, T. and Katahira, M.
  • Organizer: The 6^<th> International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Gifu
  • Year and Date: 20091104-06
• [Presentation] Structure and real-time monitoring of the enzymatic reaction of APOBEC3G which is involved in anti-HIV activity (2009)
  • Author(s): Furukawa, A., Nagata, T., Matsugami, A., Habu, Y., Sugiyama, R., Hayashi, F., Kobayashi, N., Yokoyama, S., Takaku, H. and Katahira, M.
  • Organizer: The 6th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Gifu
  • Year and Date: 20091104-06
• [Presentation] Structural aspects for the function of ATP-binding ribonucleopeptide receptors (2009)
  • Author(s): Nakano, S., Fukuda, M., Mashima, T., Katahira, M. and Morii, T.
  • Organizer: The 6^<th> International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Gifu
  • Year and Date: 20091104-06
• [Presentation] Unique quadruplex structure and interaction of the RNA aptamer against bovine prion protein (2009)
  • Author(s): Mashima, T., Matsugami, A., Nishikawa, F., Nishikawa, S. and Katahira, M.
  • Organizer: Joint Symposium of the 5^<th> annual Meeting of the Oligonucleotide Therapeutics Society and the 19^<th> Antisense Symposium
  • Place of Presentation: Fukuoka
  • Year and Date: 20091104-06
• [Presentation] Structure, interaction and real-time monitoring of the enzymatic reaction of APOBEC3G which possesses anti-HIV activity (2009)
  • Author(s): Furukawa, A., Nagata, T., Sugiyama, R., Takaku, H. and Katahira, M.
  • Organizer: The 3rd Asia-Pacific NMR Symposium
  • Place of Presentation: Cheju
  • Year and Date: 20091025-28
• [Book] Encyclopedia of Biophysics,"Introduction to Nucleic Acids NMR" (2012)
  • Author(s): Katahira, M. and Mashima, T.
  • Publisher: Springer
• [Book] 日本化学会編・CSJカレントレビュー"核酸化学のニュートレンド-DNA・RNAの新たな可能性を開く""論文にみる最重要概念と革新実験データ" (2011)
  • Author(s): 片平正人
  • Total Pages: 36-40
  • Publisher: 化学同人
• [Remarks]
  • URL: http://www.iae.kyoto-u.ac.jp/bio/a-13_j.html
• [Patent(Industrial Property Rights)] Nucleic Acid-Enzyme Complex (2011)
  • Inventor(s): 片平正人
  • Industrial Property Rights Holder: 横浜国立大学長飯田嘉宏
  • Industrial Property Number: 特許、7910721
  • Acquisition Date: 2011-03-10
  • Overseas