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2011 Fiscal Year Final Research Report

Structural biology of protein-protein interactions involved in DNA replication under DNA damage

Research Project

  • PDF
Project/Area Number 21370048
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionYokohama City University

Principal Investigator

SATO Mamoru  横浜市立大学, 生命ナノシステム科学研究科, 教授 (60170784)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Hiroshi  横浜市立大学, 生命ナノシステム科学研究科, 助教 (40336590)
Project Period (FY) 2009 – 2011
KeywordsX線結晶解析
Research Abstract

DNA clamp is involved in various cellular events on DNA including DNA replication, repair and replication, and functions as a scaffold protein in those events. DNA clamp forms a ring-shaped trimer that encircles the DNA double helix and slides freely along the DNA axis. PCNA and RAD9/HUS1/RAD1(9-1-1) are DNA clamp in eukaryote. PCNA is a homo-trimer, whereas 9-1-1 is a hetero-trimer. Each subunit of 9-1-1 is composed of PCNA-like domain. However, RAD9 has an additional domain with a molecular weight of 13 kD in the C-terminus. Structure of 9-1-1 trimer of PCNA-like domains is similar to that of PCNA trimer. In this study, we prepared the C-terminal domain of RAD9CTD and performed structural analysis by small angle X-ray scattering(SAXS). Some archaea have heterotrimeric DNA clamp(PCNA1-PCNA2-PCNA3). We reveals that PCNA2-PCNA3 from Sulfolobus tokodaii forms tetrameric ring structure in solution by SAXS.

  • Research Products

    (4 results)

All 2012 2011 2010

All Journal Article (2 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Identification of a small molecule PCNA inhibitor that disrupts interactions with PIP-Box proteins and inhibits DNA replication2012

    • Author(s)
      Chandanamali Punchihewa, Akira Inoue, Asami Hishiki, Yoshihiro Fujikawa, Michele Connelly, Benjamin Evison, Youming Shao, Richard Heath, Isao Kuraoka, Patrick Rodrigues, Hiroshi Hashimoto, Takashi Yagi, & Naoaki Fujii
    • Journal Title

      J. Biol. Chem

      Volume: 287 Pages: 14289-14300

  • [Journal Article] A novel heterotetameric structure of the crenarchaeal PCNA-2PCNA3 complex2011

    • Author(s)
      Akito Kawai, Hiroshi Hashimoto, Shigesada Higuchi, Masaru Tsunoda, Mamoru Sato, Kazuo T Nakamura, & Shuichi Miyamoto
    • Journal Title

      J. Struct. Biol

      Volume: 174 Pages: 443-450

  • [Book] 翻訳後修飾のプロテオミクス(平野久、大野茂男編集)2011

    • Author(s)
      佐藤衛、萩原央記、明石知子
    • Total Pages
      234
    • Publisher
      講談社
  • [Patent(Industrial Property Rights)] 抗PAD4抗体医薬の創成2010

    • Inventor(s)
      佐藤衛,金澤智,山田道之,清水敏之
    • Industrial Property Rights Holder
      横浜市立大学,名古屋市立大学
    • Industrial Property Number
      特願、2010-185734
    • Filing Date
      2010-08-23

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Published: 2013-07-31  

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