2011 Fiscal Year Final Research Report
Single molecule FRET study for the walking mechanism of molecular motor kinesin
| Project/Area Number |
21370066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOMISHIGE Michio 東京大学, 大学院・工学系研究科, 准教授 (50361530)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 一分子計測 / 蛍光共鳴エネルギー移動 / 分子機械 / 分子モーター / 構造変化 |
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| Research Abstract |
Molecular motor kinesin walks along microtubules by alternately moving two motor heads. We hypothesized based on atomic structural models that the neck linkers connecting two heads are essential for the coordination between heads. We tested this hypothesis by observing at the single molecule level the movements and structural changes of the mutant kinesins whose tension posed to the neck linker were reduced or abolished and provided the structural basis to explain the walking mechanism of kinesin.
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