2011 Fiscal Year Final Research Report
Investigation of the folding dynamics of proteins based on the advanced method of single molecule detection
| Project/Area Number |
21370072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Research Collaborator |
KAMAGATA Kiyoto 東北大学, 多元物質科学研究所, 助教 (90432492)
OIKAWA Hiroyuki 東北大学, 多元物質科学研究所, 教育研究支援者
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| Project Period (FY) |
2009 – 2011
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| Keywords | フォールディング |
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| Research Abstract |
In this project, we first developed spherical mirror system for the detection of fluorescence from single molecules for long periods. Based on the developed system, we successfully determined the energy landscape for cytochrome c. We next developed line conforcal microscopy for the fast time resolved measurements of single molecule fluorescence time series. The method was used to investigate the unfolded state dynamics of the B domain of protein A, and revealed the presence of substates in the unfolded ensemble. Further applications of the developed methods for other protein systems are currently underway.
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