2011 Fiscal Year Final Research Report
Studies by molecular biological methods on proton pumping mechanisms of bovine heart and bacterial cytochrome c oxidases
| Project/Area Number |
21370073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Hyogo |
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Principal Investigator |
SHIMADA Hideo 兵庫県立大学, 大学院・生命理学研究科, 教授 (80095611)
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| Co-Investigator(Renkei-kenkyūsha) |
KATAYAMA Yukie 兵庫県立大学, 大学院・生命理学研究科, 特任講師 (70464998)
AMINAKA Ryohta 兵庫県立大学, 大学院・生命理学研究科, 特任助教
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| Project Period (FY) |
2009 – 2011
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| Keywords | タンパク質 / 核酸の構造 / 動態 / 機能 |
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| Research Abstract |
Function of a putative proton transfer pathway, D-pathway of bovine heart cytochrome c oxidase (CcO) was analyzed by site-directed mutagenesis employing HeLa cell expression system we have developed to produce bovine/human hybrid CcO. Corresponding bacterial pathway has been proposed to convey pumping and water-forming protons, which is supported by mutagenesis. Our analyses did not support the proton pumping function. Study on proton transfer mechanism of proteins by vibrational spectroscopy requires site-specific isotope labeling of proteins, which can be achieved by cell-free protein synthesis. We have successfully developed a E. coli cell free system to produce normal bacterial CcO.
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