Study on the role of Rab33 in membrane trafficking

2011 Fiscal Year Final Research Report

• Project/Area Number: 21370087
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tohoku University
• Principal Investigator: FUKUDA Mitsunori 東北大学, 大学院・生命科学研究科, 教授 (50311361)
• Project Period (FY): 2009 – 2011
• Keywords: Rab33 / エフェクター / 膜輸送 / Atg16L1 / オートファジー / 低分子量G蛋白質 / オルガネラ / 神経細胞

Research Abstract

Small GTPase Rab33 is mostly conserved in vertebrates and has been suggested to be involved in specific membrane trafficking events, although its precise role remained to be determined. In this study, we identified Atg16L and RUFY2/3 as novel GTP-Rab33-specific binding proteins and demonstrated the possible involvement of Rab33 in the regulation of autophagosomal maturation and neuronal function.

Research Products

• [Journal Article] Atg16L2, a novel isoform of mammalian Atg16L that is not essential for canonical autophagy despite forming an Atg12. 5-16L2 complex. (2011)
  • Author(s): Ishibashi, K., Fujita, N., Kanno, E., Omori, H., Yoshimori, T., Itoh, T. & Fukuda, M.
  • Journal Title: Autophagy Volume: 7 Pages: 1500-1513
  • Peer Reviewed
• [Journal Article] A possible role of Atg8 homologues as a scaffold for signal transduction (2011)
  • Author(s): Itoh, T. & Fukuda, M.
  • Journal Title: Autophagy Volume: 7 Pages: 1080-1081
  • Peer Reviewed
• [Journal Article] Genome-wide investigation of the Rab binding activity of RUN domains : Development of a novel tool that specifically traps GTP-Rab35 (2011)
  • Author(s): Fukuda, M., Kobayashi, H., Ishibashi, K. & Ohbayashi, N.
  • Journal Title: Cell Struct. Funct Volume: 36 Pages: 155-170
  • Peer Reviewed
• [Journal Article] OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation (2011)
  • Author(s): Itoh, T., Kanno, E., Uemura, T., Waguri, S. & Fukuda, M.
  • Journal Title: J. Cell Biol Volume: 192 Pages: 839-853
  • Peer Reviewed
• [Journal Article] TBC proteins : GAPs for mammalian small GTPase Rab? (2011)
  • Author(s): Fukuda, M.
  • Journal Title: Biosci. Rep Volume: 31 Pages: 159-168
  • Peer Reviewed
• [Journal Article] Comprehensive screening for novel Rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs (2010)
  • Author(s): Kanno, E., Ishibashi, K., Kobayashi, H., Matsui, T., Ohbayashi, N. & Fukuda, M.
  • Journal Title: Traffic Volume: 11 Pages: 491-507
  • Peer Reviewed
• [Presentation] Rabの網羅的解析ツールを用いた新規膜輸送経路の探索と新たなRab機能の同定 (2011)
  • Author(s): 福田光則
  • Organizer: 第84回日本生化学会大会シンポジウム「メンブレントラフィック研究の最近のトレンド(低分子量GTPaseによる多彩な制御機構)」
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] オートファジーに関与するRab-GAPの探索 (2010)
  • Author(s): 伊藤敬、菅野栄子、植村武文、和栗聡、福田光則
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] オートファジー制御に関与する低分子量GTPase Rabの機能解析 (2009)
  • Author(s): 福田光則
  • Organizer: 第82回日本生化学会大会シンポジウム「低分子量GTPaseから眺めるメンブレントラフィック研究の新展開」
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Remarks]
  • URL: http://www.lifesci.tohoku.ac.jp/research/field_list/membrane_trafficking/t_fukuda