Evolution of W, female-specific sex chromosome through the comparative genomics between Bombyx mori and B. mandarina

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: KADONO-OKUDA Keiko 独立行政法人農業生物資源研究所, 昆虫ゲノム研究ユニット, 上級研究員 (40355722)
• Co-Investigator(Kenkyū-buntansha): MITA Kazuei (独)農業生物資源研究所, 昆虫科学研究領域, 特任上級研究員 (30159165) ; ABE Hiroaki 東京農工大学, 大学院・共生科学技術研究院, 助教 (80222660) ; MAEKAWA Hideaki 琉球大学, 熱帯生物圏研究センター, 教授 (60100096) ; NAKAJIMA Yumiko 琉球大学, 熱帯生物圏研究センター, 准教授 (70244340) ; YUKUHIRO Kenji (独)農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (50343992)
• Co-Investigator(Renkei-kenkyūsha): SHIMADA Toru 東京大学, 農学生命科学研究科, 教授 (20202111)
• Project Period (FY): 2009 – 2011
• Keywords: 養蚕 / 蚕糸

Research Abstract

We analyzed the W-chromosome construction in B. mori, B. mandarina and other related species. B. mandarina and B. huttoni was branched 14 million years ago(MYA). In B. mandarina one copy set of two genes was translocated from an autosome to W-chromosome(z1-z20) in 7 MYA, and z1-z20 was duplicated on W-chromosome 0.3 MYA. Such a new type of B. mandarina containing duplicated z1-z20 on W-chromosome had spread drastically. But, since Japan islands have been isolated from the continent 0.1 MYA, the new type did not invade in Japan. In present, there are 4 copies of z1-z20 in all B. mori, and B. mandarina except for Japan.

Research Products

• [Journal Article] Female sex pheromone and male behavioral responses of the bombycid moth Trilocha varians : comparison with those of the domesticated silkmoth Bombyx mori (2012)
  • Author(s): Daimon T, Fujii T, Yago M, Hsu Y-F, Nakajima Y, Fujii T, Katsuma S, Ishikawa Y, and Shimada T
  • Journal Title: Naturwissenschaften Volume: 99 Pages: 207-15
  • Peer Reviewed
• [Journal Article] Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth (2012)
  • Author(s): Daimon T, Yago M, Hsu Y-F., Fujii T, Nakajima Y, Kokusho R, Abe H, Katsuma S, Shimada T
  • Journal Title: Trilocha varians.、Journal of Insect Science Volume: 12
  • Peer Reviewed
• [Journal Article] The silkworm W chromosome is a source of female-enriched piRNAs (2012)
  • Author(s): Kawaoka S, Kadota K, Arai Y, Suzuki Y, Fujii T, Abe H, Yasukochi Y, Mita K, Sugano S, Shimizu K, Tomari Y, Shimada T, Katsuma Shimada, T.
  • Journal Title: RNA Volume: 17 Pages: 2144-51
  • Peer Reviewed
• [Journal Article] 南西諸島に棲息する昆虫・クモ類における転移因子マリナー様配列(MLE : mariner-like element)の比較系統解析 (2011)
  • Author(s): 山田香織,川西祐一,奥間政一郎,岩崎華子,山田明徳.佐々木健志,中島裕美子,前川秀彰
  • Journal Title: Entomotech Volume: 35 Pages: 13-39
  • Peer Reviewed
• [Journal Article] 分子系統解析によるクワコの進化と日本列島の地理的変動との関係-カイコのゲノム情報とクワコの多型から- (2010)
  • Author(s): 川西祐一、前川秀彰、中島裕美子
  • Journal Title: 蚕糸・昆虫バイオテック Volume: 79 Pages: 109-117
• [Journal Article] Novel non-autonomous transposable elements on W chromosome of the silkworm (2010)
  • Author(s): Abe H, Fujii T, Shimada T, Mita K.
  • Journal Title: Bombyx mori., Journal of Genetics Volume: 89 Pages: 375-87
  • Peer Reviewed
• [Journal Article] Molecular analysis of sex chromosome-linked mutants in the silkworm Bombyx mori (2010)
  • Author(s): Fujii T, Abe H, Shimada T.
  • Journal Title: Journal of Genetics Volume: 53 Pages: 585-93
  • Peer Reviewed
• [Journal Article] Recent transposition of yabusame, a novel piggyBac-like transposable element in the genome of the silkworm, Bombyx mori (2010)
  • Author(s): Daimon T, Mitsuhiro M, Katsuma S, Abe H, Mita K, Shimada T.
  • Journal Title: Genome Volume: 53 Pages: 585-93
  • Peer Reviewed
• [Journal Article] Repression of tyrosine hydroxylase is responsible for the sex-linked chocolate mutation of the silkworm, Bombyx mori (2010)
  • Author(s): Liu C, Yamamoto K, Cheng TC, Kadono-Okuda K, Narukawa J, Liu SP, Han Y, Futahashi R, Kidokoro K, Noda H, Kobayashi I, Tamura T, Ohnuma A, Banno Y, Dai FY, Xiang ZH, Goldsmith MR, Mita K, Xia QY
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 12980-5
  • Peer Reviewed
• [Journal Article] 亜熱帯に生息する昆虫・クモ類に見出される転移因子マリナー様配列の水平伝播機構の解析 (2010)
  • Author(s): 奥間政一郎,川西祐一,佐々木健志,日高道雄,前川秀彰,中島裕美子
  • Journal Title: Entomotech Volume: 34 Pages: 47-52
  • URL: http://www.agr.kyushu-u.ac.jp/sanshi-kyushu/entomotech34.html
  • Peer Reviewed
• [Journal Article] Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable(spli) larval body phenotype in the silkworm (2010)
  • Author(s): Fujii T, Kuwazaki S, Yamamoto K, Abe H, Ohnuma A, Katsuma S, Mita K, Shimada T.
  • Journal Title: Bombyx mori. Genome Volume: 53 Pages: 45-54
  • Peer Reviewed
• [Journal Article] Identification of 2nd chromosome region translocated onto the W chromosome by RFLP with EST-cDNA clones in the Gensei-kouken strains of the mulberry silkworm (2010)
  • Author(s): Sivaramakurup Sreekumar, Keiko Kadono-Okuda, Ken-ichi Nagayasu and Wajiro Hara
  • Journal Title: Bombyx mori L、Genetics and Molecular Biology Volume: 33 Pages: 27-35
  • Peer Reviewed
• [Presentation] カイコおよび近縁野蚕ゲノムにおけるBm1について (2012)
  • Author(s): 味村正博、三田和英, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
  • Year and Date: 20120305-06
• [Presentation] カイコ濃核病ウイルスと抵抗性突然変異の起源 (2012)
  • Author(s): 門野敬子、味村正博、三田和英、中島裕美子、前川秀彰、行弘研司、阿部広明, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
  • Year and Date: 20120305-06
• [Presentation] 日本産クワコの系統地理学 (2012)
  • Author(s): 行弘研司, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
  • Year and Date: 20120305-06
• [Presentation] カイコとクワコのBmTNML座位における、転移因子の動向 (2012)
  • Author(s): 中島裕美子, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
  • Year and Date: 20120305-06
• [Presentation] Molecular genetics of the densovirus resistance genes in Bombyx silkworms (2011)
  • Author(s): K. Kadono-Okuda, D. O. Ogoyi, H. Masui, K. Kidokoro, M. Ajimura, K. Ito, K. Yukuhiro, E. Kosegawa, Y. Banno, H. Maekawa, Y. Nakajima, K. Morimoto, M. Ichida, T. Adachi, K. Yamamoto, K. Mita
  • Organizer: 22nd Congress of the International Sericultural Commission
  • Place of Presentation: Chiangmai(タイ)
  • Year and Date: 20111214-17
• [Presentation] (パスツール賞受賞講演) Silk Genome Opens New Silk Road for Insect Sciences and Its Applications (2011)
  • Author(s): K. Mita
  • Organizer: 22nd Congress of the International Sericultural Commission
  • Place of Presentation: Chiangmai(タイ)
  • Year and Date: 20111214-17
• [Presentation] Ancient Mariner Transposon Targeted by Other Transposable Elements in the Genome of Bombyx mori and B. mandarina (2011)
  • Author(s): Nakagome, S., Mano, S., Nakajima, Y.
  • Organizer: Annual Meeting of the Society for Molecular Biology and Evolution
  • Place of Presentation: 京都大学
  • Year and Date: 20110700