2011 Fiscal Year Final Research Report
Molecular analysis of novel factors involved in repression of infection and propagation of a DNA virus in silkworm.
| Project/Area Number |
21380043
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
TANAKA Hiromitsu 独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (30391577)
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| Co-Investigator(Kenkyū-buntansha) |
SEZUTSU Hideki 独立行政法人農業生物資源研究所, 遺伝子組換え研究開発ユニット, ユニット長 (70342805)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 昆虫病理 |
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| Research Abstract |
RNA interference and overexpression experiments using silkworm culture cells demonstrated that an Ets family transcription factor, BmEts was involved in repression of propagation of BmNPV, which belongs to DNA viruses, in the silkworm cells. BmEts suppressed the activation of promoter activity of viral immediate early gene. Furthermore, the expression of BmEts gene was shown to be up-regulated in response to bacterial challenge. BmEts enhanced the expression of a silkworm antimicrobial peptide gene, lebocin. On the other hand, BmToll10-3was shown to be involved in the promoter activation of viral immediate early gene, suggesting that BmToll10-3induces activation of transcription factors for enhancement of the immediate early gene promoter activity, in contrast to BmEts. We believe that these findings contribute to elucidation of the mechanisms of insect immunity against DNA viruses.
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