2011 Fiscal Year Final Research Report
Study on the Structures, Functions and Applications of Sphingolipid-metabolizing machinery
| Project/Area Number |
21380066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
ITO Makoto 九州大学, 大学院・農学研究院, 教授 (40253512)
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| Co-Investigator(Kenkyū-buntansha) |
OKINO Nozomu 九州大学, 大学院・農学研究院, 准教授 (90363324)
KAKUTA Yoshimitsu 九州大学, 大学院・農学研究院, 准教授 (00314360)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 糖鎖生物学 / 酵素利用学 / スフィンゴ(糖)脂質 / 代謝 / 結晶構造 |
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| Research Abstract |
In this study, we explored the structures and functions of novel sphingolipid-metabolizing enzymes(ceramidase, sphingolipid ceramide N-deacylase, Hex1, NgaP, EGCrP1, EGALC), which had been identified and cloned in our laboratory. Interestingly, both ceramidase and sphingolipid ceramide N-deacylase are a zinc-dependent amidohydrolase. The cleavage of N-acyl linkage of ceramide by ceramidase follows a similar mechanism as observed for the zinc-dependent carboxypeptidases. Hex1 and NgaP were found to cleave the terminal
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