Identification of membrane proteins associated with Prion invasion in bovine intestinal microfold (M)cells

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380170
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Tohoku University
• Principal Investigator: ASO Hisashi 東北大学, 大学院・農学研究科, 教授 (50241625)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Takahiro 東北大学, 大学院・農学研究科, 教授 (20111297) ; OHWADA Shuichi 東北大学, 大学院・農学研究科, 助教 (00183244) ; WATANABE Koichi 東北大学, 大学院・農学研究科, 助教 (80261494) ; KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885)
• Project Period (FY): 2009 – 2011
• Keywords: 機能性物質

Research Abstract

In order to identify a membrane protein associated with the Prion invasion in bovine intestinal microfold (M)cells, M cell-differentiated bovine intestinal epitheliocyte cell line (BIE cells)were treated with recombinant bovine prion protein-coated magnetic beads, and then vesicles including magnetic beads were purified from BIE cells. After 2D-electrophoresis, fructose-bisphosphate aldolase A was identified using LC-MS/MS spectrometer. Aldolase A were specifically expressed on M cells in ileal Peyer's patches and the epithelium in myctero pharynges, these tissues of which were thought to be associated with Prion invasion. In addition, the far western analysis revealed that aldolase A could bind to prion protein.

Research Products

• [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway. (2012)
  • Author(s): Tomoyuki Shimazu, Julio Villena, Masanori Tohno, Hitomi Fujie, Shoichi Hosoya, Takeshi Shimosato, Hisashi Aso, Yoshihito Suda, Yasushi Kawai, Tadao Saito, Seiya Makino, Shuji Ikegami, Hiroyuki Itoh, Haruki Kitazawa.
  • Journal Title: Infection and Immunity Volume: 80 (1) Pages: 276-288
  • Peer Reviewed
• [Journal Article] Orally administered prion protein is incorporated by M cells and propagated into lymphoid tissues by macrophages in prion protein knockout mice. (2011)
  • Author(s): Ikuro Takakura, Kohtaro Miyazawa, Takashi Kanaya, Wataru Itani, Kouichi Watanabe, Shyuichi Ohwada, Hitoshi Watanabe, Michael T. Rose, Suehiro Sakaguchi, Noriyuki Nishida, Shigeru Katamine, Takahiro Yamaguchi, Hisashi Aso.
  • Journal Title: American Journal of Pathology Volume: 179(3) Pages: 1301-1309
  • Peer Reviewed
• [Journal Article] Cytokeratin 18 is a specific marker of bovine intestinal microfold (M)cell. (2011)
  • Author(s): Tetsuya Hondo, Takashi Kanaya, Ikuro Takakura, Hitoshi Watanabe, Shyuichi Ohwada, Kouichi Watanabe, Haruki Kitazawa, Michael T. Rose, Takahiro Yamaguchi, Hisashi Aso.
  • Journal Title: American Journal of Physiology-Gastrointestinal and Liver Physiology Volume: 300 Pages: G442-G453
  • Peer Reviewed
• [Journal Article] Role of peripheral serotonin in glucose and lipid metabolism. (2011)
  • Author(s): Hitoshi Watanabe, Michael T. Rose, Hisashi Aso.
  • Journal Title: Current Opinion in Lipidology Volume: 22 Pages: 186-191
  • Peer Reviewed
• [Journal Article] TIEG1 negatively controls the myoblast pool indispensable for fusion during myogenic differentiation of C2C12 cells. (2010)
  • Author(s): Miyake M., Hayashi Shinichiro, Iwasaki S., Uchida T., Watanabe K., Ohwada S., Aso H., Yamaguchi T.
  • Journal Title: Journal of Cellular Physiology Volume: 226(4) Pages: 1128-1136
  • Peer Reviewed
• [Journal Article] Peripheral serotonin enhances lipid metabolism by accelerating bile acid turnover. (2010)
  • Author(s): Hitoshi Watanabe, Daisuke Akasaka, Hideki Ogasawara, Kan Sato, Masato Miyake, Kazuki Saito, Takashi Kanaya, Ikuro Takakura, Guozheng Chao, Michael T. Rose, Shyuichi Ohwada, Kouichi Watanabe, Takahiro Yamaguchi, Hisashi Aso.
  • Journal Title: Endocrinology Volume: 151(10) Pages: 4776-4786
  • Peer Reviewed
• [Journal Article] Transcytosis of murine-adapted BSE agent in an in vitro bovine M cell model. (2010)
  • Author(s): Kohtaro Miyazawa, Takashi Kanaya, Ikuro Takakura, Sachi Tanaka, Tetsuya Hondo, Hitoshi Watanabe, Michael T. Rose, Haruki Kitazawa, Takahiro Yamaguchi, Shigeru Katamine, Noriyuki Nishida, Hisashi Aso.
  • Journal Title: Journal of Virology Volume: 84(23) Pages: 12285-12291
  • Peer Reviewed
• [Journal Article] Possible role of TIEG1 as a feedbaclk regulator of myostatin and TGF-β in myoblasts. (2010)
  • Author(s): Miyake M., Hayashi S., Iwasaki S., Chao G., Takahashi H., Watanabe K., Ohwada S., Aso H., Yamaguchi T.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 393(4) Pages: 762-766
  • Peer Reviewed
• [Journal Article] ウシ腸管上皮細胞株(BIE)のin vitro M細胞分化誘導系における最適条件の検討 (2010)
  • Author(s): 本堂哲也、金谷高史、高橋遊、渡邉一史、大和田修一、渡邊康一、山口高弘、麻生久
  • Journal Title: 東北畜産学会報 Volume: 55(3) Pages: 49-53
  • Peer Reviewed
• [Journal Article] Characterization of newly established bovine intestinal epithelial cell line. (2010)
  • Author(s): Kohtaro Miyazawa, Tetsuya Hondo, Takashi Kanaya, Sachi Tanaka, Ikuro Takakura, Wataru Itani, Michael T. Rose, Haruki Kitazawa, Takahiro Yamaguchi, Hisashi Aso.
  • Journal Title: Histochemistry and Cell Biology Volume: 133 Pages: 125-134
  • Peer Reviewed
• [Journal Article] Utilization of the porcine system to study lymphotoxin-β regulation in intestina lymphoid tissue. (2009)
  • Author(s): Tomoyuki Shimazu, Masanori Tohno, Shinichiro Katoh, Takeshi Shimosato, Hisashi Aso, Yasushi Kawai, Tadao Saito, Haruki Kitazawa.
  • Journal Title: Biochemical Genetics Volume: 47 Pages: 126-136
  • Peer Reviewed
• [Journal Article] Expression of Hyaluronan Synthase 1 and Distribution of Hyaluronan During Follicular Atresia in Pig Ovaries. (2009)
  • Author(s): Yuko Miyake, Masahiro Sakurai, Sachi Tanaka, Woro Anindito Sri Tunjung, Masaki Yokoo, Hiromichi Matsumoto, Hisashi Aso, Takahiro Yamaguchi, and Eimei Sato.
  • Journal Title: Biology of Reproduction Volume: 80(2) Pages: 249-257
  • Peer Reviewed
• [Presentation] Effect of peripheral serotonin on glucose uptake in murine brown adipocyte (2011)
  • Author(s): Watanabe H., Saito K., Saito R., Nakano T., Okada N., Sumiyoshi K., Ohwada S., Watanabe K., Aso H
  • Organizer: 2011 ASCB Annual Meeting. 11th-15th December The American Society for Cell Biology
  • Place of Presentation: Denver, USA
  • Year and Date: 20111211-15
• [Presentation] 腸管からのプリオン侵入機構 (2011)
  • Author(s): 麻生久
  • Organizer: 第16回東北大学学際ライフサイエンスシンポジウム
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2011-11-01
• [Presentation] 腸管上皮M細胞は経口摂取プリオン蛋白の取り込みに関与する (2011)
  • Author(s): 麻生久
  • Organizer: 日本解剖学会第57回東北・北海道連合支部学術集会
  • Place of Presentation: 岩手大学(盛岡)
  • Year and Date: 2011-09-11
• [Presentation] Evaluation of anti-inflammatory immunobiotics in porcine intestinal epithelial cell (2010)
  • Author(s): Kitazawa, Haruki ; Shimazu, Tomoyuki ; Tohno, Masanori ; Villena, Julio ; Hosoya, Syoichi ; Fujie, Hitomi ; Suda, Yoshihito ; Shimosato, Takeshi ; Aso, Hisashi ; Makino, Seiya ; Ikegami, Shuuji ; Kawai, Yasushi ; Saito, Tadao
  • Organizer: International Scientific Conference on Probiotics and Prebiotics-IPC2010
  • Place of Presentation: Kosice, Slovakia
  • Year and Date: 20100615-17
• [Presentation] Effect of immunobiotics on the immune performance and productivity of pigs (2010)
  • Author(s): Suda, Yoshihito ; Takahashi, Yu ; Tohno, Masanori ; Villena, Julio ; Hosoya, Syoichi ; Fujie, Hitomi ; Shimosato, Takeshi ; Kobashi, Jin ; Ishida, Mitsuharu ; Aso, Hisashi ; Kawai, Yasushi ; Saito, Tadao ; Makino, Seiya ; Ikegami, Shuuji ; Kitazawa, Haruk
  • Organizer: International Scientific Conference on Probiotics and Prebiotics-IPC2010
  • Place of Presentation: Kosice, Slovakia
  • Year and Date: 20100615-17
• [Presentation] Peripheral Serotonin Enhanced Bile Acids Circulation and Accelerated Lipid Metabolism (2009)
  • Author(s): H. Watanabe, D. Akasaka, K. Saito, H. Ogasawara, T. Kanaya, M. Miyake, S. Iwasaki, T. Hondo, Y. Takahashi, K. Sato, S. Ohwada, K. Watanabe, T. Yamaguti, H. Aso
  • Organizer: 49th Annual Meeting, The American Society for Cell Biology
  • Place of Presentation: San Diego, U. S. A
  • Year and Date: 20091205-10
• [Presentation] Myostatin Expresses in Neurons Related to Olfactory System in the Brain (2009)
  • Author(s): S. Iwasaki, M. Miyake, E. Kitagawa, K. Watanabe, S. Ohwada, H. Aso, T. Yamaguti
  • Organizer: 49th Annual Meeting, The American Society for Cell Biology
  • Place of Presentation: San Diego, U. S. A
  • Year and Date: 20091205-10
• [Presentation] Silencing TIEG1 Expression Promotes Myotube Formation through Regulation of Apoptosis (2009)
  • Author(s): M. Miyake, S. Iwasaki, C. Guozheng, H. Takahashi, K. Watanabe, S. Ohwada, H. Aso, T. Yamaguti
  • Organizer: 49th Annual Meeting, The American Society for Cell Biology
  • Place of Presentation: San Diego, U. S. A
  • Year and Date: 20091205-10
• [Presentation] Cytokeratin 18 Is a Specific Marker of M Cell of Bovine Peyer's Patches (2009)
  • Author(s): T. Hondo, H. Aso, T. Kanaya, I. Takakura, H. Watanabe, M. Miyake, S. Iwasaki, K. Saito, Y. Takahashi, S. Ohwada1, K. Watanabe, T. Yamaguti
  • Organizer: 49th Annual Meeting, The American Society for Cell Biology
  • Place of Presentation: San Diego, U. S. A
  • Year and Date: 20091205-10
• [Book] The Expression of Cytokeratins in Bovine Intestinal Microfold (M)Cells. In : Cytokeratins-Tools in Oncology (2012)
  • Author(s): Takashi Kanaya, Tetsuya Hondo, Kohtaro Miyazawa, Michael T. Rose and Hisashi Aso
  • Total Pages: 3-14
  • Publisher: In Tech
• [Book] 農学生命科学を学ぶための入門生物学 (2011)
  • Author(s): 麻生久
  • Total Pages: 21-25
  • Publisher: 東北大学出版会
• [Book] Phenotypes and Distributions of Dendritic Cells in Peripheral Blood and Tissues Cattle (2010)
  • Author(s): Kohtaro Miyazawa, Takashi, Kanaya, Sachi Tanaka, Haruki Kitazawa, Michael T. Rose, Takahiro Yamaguchi, Hisashi Aso
  • Total Pages: 123-138
  • Publisher: NOVA Science Publishers, Inc.
• [Remarks]
  • URL: http://www.agri.tohoku.ac.jp/keitai/index-j.html