Nobel method to transform plants through epigenetic modifications by the cucumber mosaic virus-based vector

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380203
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Hokkaido University
• Principal Investigator: MASUTA Chikara 北海道大学, 大学院・農学研究院, 教授 (60281854)
• Co-Investigator(Kenkyū-buntansha): KANAZAWA Akira 北海道大学, 大学院・農学研究院, 准教授 (30281794)
• Project Period (FY): 2009 – 2011
• Keywords: 遺伝子 / 染色体工学

Research Abstract

Gene silencing through transcriptional repression can be induced by targeting double-stranded RNA (dsRNA) to a gene promoter. We show that heritable gene silencing can be induced by targeting dsRNA to the endogenous gene promoters in petunia and tomato plants, using the Cucumber mosaic virus (CMV)-based vector. This CMV-based gene silencing system provides a useful tool to artificially modify DNA methylation in plant genomes and elucidate the mechanism for epigenetic controls.

Research Products

• [Journal Article] The 2b proteins of Cucumber mosaic virus generally have the potential to differentially induce necrosis on Arabidopsis (2012)
  • Author(s): Masuta, C., Inaba, J. and Shimura, H.
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 43-45
  • Peer Reviewed
• [Journal Article] Decrease in Globodera rostochiensis (Woll.) (Nematode:Heteroderidae) population density on resistant tomato cultivars in greenhouse trials (2011)
  • Author(s): Uehara, T., Itou, K., Narabu, T. and Masuta, C.
  • Journal Title: Nematological Research Volume: 41 Pages: 41-44
  • Peer Reviewed
• [Journal Article] Tobacco salicylic acid glucosyltransferase is active toward tuberonic acid (12-hydroxyjasmonic acid) and is induced by mechanical wounding stress (2011)
  • Author(s): Seto, Y., Hamada, S., Ito, H., Masuta, C., Matsui, H., Nabeta, K. and Matsuura, H.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Pages: 2316-2320
  • Peer Reviewed
• [Journal Article] Variability in the P1 gene helps to refine phylogenetic relations among Leek yellow stripe virus isolates from garlic (2011)
  • Author(s): Yoshida, N, Shimura, H., Yamashita, K., Suzuki, M. and Masuta, C.
  • Journal Title: Archives of Virology Volume: 157 Pages: 147-153
  • Peer Reviewed
• [Journal Article] Infection dynamics in viral spread and interference under the synergism between Cucumber mosaic virus and Turnip mosaic virus (2011)
  • Author(s): Takeshita, M., Koizumi, E., Noguchi, M., Sueda, K., Shimura, H., Ishikawa, N., Matsuura, H, Ohshima, K., Natsuaki, T., Kuwata, S., Furuya, N., Tsuchiya, K. and Masuta, C.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Pages: 18-27
  • Peer Reviewed
• [Journal Article] Virus-induced necrosis Is a consequence of direct protein-protein interaction between a viral RNA-silencing suppressor and a host catalase (2011)
  • Author(s): Inaba, J., Kim, B. M., Shimura, H. and Masuta, C.
  • Journal Title: Plant Physiology Volume: 156 Pages: 2026-2036
  • Peer Reviewed
• [Journal Article] Size and positional effects of promoter RNA segments on virus-induced RNA-directed DNA methylation and transcriptional genesilencing (2011)
  • Author(s): Otagaki, S., Kawai, M., Masuta, C. and Kanazawa, A.
  • Journal Title: Epigenetics Volume: 6 Pages: 681-691
  • Peer Reviewed
• [Journal Article] Combinations of a host resistance gene and the CI gene of Turnip mosaic virus differentially regulate sympotom expression in Brassica rapa cultivars (2011)
  • Author(s): Fujiwara, A., Inukai, T., Kim, B. M. and Masuta, C.
  • Journal Title: Archives of Virology Volume: 156 Pages: 1575-1581
  • Peer Reviewed
• [Journal Article] RNA-mediated epigenetic modifications of an endogenous gene targeted by a viral vector:a potent gene silencing system to produce a plant that does not carry atransgene but has altered traits (2011)
  • Author(s): Kanazawa, A., Inaba, J., Kasai, M., Shimura, H. and Masuta, C.
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 1090-1093
  • Peer Reviewed
• [Journal Article] A viral satellite RNA induces yellow symptoms on tobacco by targeting a gene involved in chlorophyll biosynthesis using the RNA silencing machinery (2011)
  • Author(s): Shimura, H., Pantaleo, V., Ishihara, T., Myojo, N., Inaba, J., Sueda, K., Burgyan, J. and Masuta, C.
  • Journal Title: PLoS Pathogens Volume: 7(5) Pages: e1002021
  • Peer Reviewed
• [Journal Article] Distal transport of exogenously applied jasmonoyl-isoleucine with wounding stress (2011)
  • Author(s): Sato, C., Aikawa, K., Sugiyama, S., Nabeta, K., Masuta, C. and Matsuura, H.
  • Journal Title: Plant Cell Physioogy Volume: 52 Pages: 509-517
  • Peer Reviewed
• [Journal Article] Multigenic system controlling viral systemic infection determined by the interactions between Cucumber mosaic virus genes and QTLs of soybean cultivar (2011)
  • Author(s): Ohnishi, S., Echizenya, I., Yoshimoto, Kim, B. M., E., Inukai, T. and Masuta, C.
  • Journal Title: Phytopathology Volume: 101 Pages: 575-582
  • Peer Reviewed
• [Journal Article] Virus induced gene silencing in Antirrhinum majus using the Cucumber mosaic virus vector : Functional analysis of the AINTEGUMENTA (Am-ANT) gene of A. majus (2011)
  • Author(s): Kim, B. M., Inaba, J. and Masuta, C.
  • Journal Title: Horticulture, Environment, and Biotechnology Volume: 52 Pages: 176-182
  • Peer Reviewed
• [Journal Article] Virus-mediated efficient induction of epigenetic modifications of endogenous genes with phenotypic changes in plants (2010)
  • Author(s): Kanazawa, A., Inaba, J., Shimura, H., Otagaki, S., Tsukahara, S., Matsuzawa, A., Kim, B., Goto, K., Masuta, C.
  • Journal Title: The Plant Journal Volume: 65 Pages: 156-68
  • Peer Reviewed
• [Journal Article] Resistant and Susceptible Responses in Tomato to Cyst Nematode are Differentially Regulated by Salicylic Acid (2010)
  • Author(s): Uehara, T., Sugiyama, S., Matsuura, H., Arie, T. and Masuta, C.
  • Journal Title: Plant Cell Physiology Volume: 52 Pages: 1524-1536
  • Peer Reviewed
• [Journal Article] Risk-managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement (2010)
  • Author(s): Fukuzawa, N., Ishihara, T., Itchoda, N., Tabayashi, N., Kataoka, C., Masuta, C. and Matsumura, T.
  • Journal Title: Plant Biotechnology Journal Volume: 9 Pages: 38-49
  • Peer Reviewed
• [Journal Article] Soybean stem growth habit gene Dt1 is an orthologue of Arabidopsis TFL1 (2010)
  • Author(s): Liu, B., Watanabe, S., Uchimiya, T., Kong, F., Kanazawa, A., Xia, Z., Nagamatsu, A., Arai, M., Yamada, T., Kitamura, K., Masuta, C., Harada, K. and Abe, J.
  • Journal Title: Plant Physiology Volume: 153 Pages: 198-210
  • Peer Reviewed
• [Journal Article] HC-Pro, a potyvirus RNA silencing suppressor, cancels cycling of Cucumber mosaic virus in Nicotiana benthamiana plants (2010)
  • Author(s): Fukuzawa, N., Itchoda, N., Ishihara, T., Goto, K., Masuta, C. and Matsumura, T.
  • Journal Title: Virus Genes Volume: 40 Pages: 440-446
  • Peer Reviewed
• [Journal Article] The C-terminal residues of the 2b protein of Cucumber mosaic virus are important for efficient expression in Escherichia coli and DNA binding (2010)
  • Author(s): Sueda, K., Shimura, H., Meguro, A., Uchida T., Inaba J., and Masuta, C.
  • Journal Title: FEBS Letters Volume: 584 Pages: 945-950
  • Peer Reviewed
• [Journal Article] A progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its RNA silencing suppressor leading to systemic infection in tobacco (2010)
  • Author(s): Asaoka, R., Shimura, H., Arai, M. and Masuta, C.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 32-339
  • Peer Reviewed
• [Journal Article] Mixed Infection in Tomato to Ensure Frequent Generation of A Natural Reassortant Between Two Subgroups of Cucumber mosaic virus (2010)
  • Author(s): Maoka, T., Saito-Hayano, Y., Iwasaki, M., Yoshida, K. and Masuta, C.
  • Journal Title: Virus Genes Volume: 40 Pages: 148-150
  • Peer Reviewed
• [Journal Article] The P3 Protein of turnip mosaic virus can alone induce HR-like cell death in Arabidopsis thaliana carrying TuNI (2010)
  • Author(s): Kim, B., Suehiro, N., Natsuaki, T., Inukai, T. and Masuta, C.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 40 Pages: 144-152
  • Peer Reviewed
• [Journal Article] Purification and cDNA cloning of a wound inducible glucosyltransferase active toward 12-hydroxy jasmonic acid (2009)
  • Author(s): Seto, Y., Hamada, S., Matsuura, H., Matsushige, M., Satou, C., Takahashi, K., Masuta, C., Ito, H., Matsui, H. and Nabeta, K.
  • Journal Title: Phytochemistry Volume: 70 Pages: 370-379
  • Peer Reviewed
• [Journal Article] Simultaneous UPLC MS/MS analysis of endogenous jasmonic acid, salicylic acid, and their related compounds (2009)
  • Author(s): Matsuura, H., Aoi, A., Satou, C., Nakaya, M., Masuta, C. and Nabeta, K.
  • Journal Title: Plant Growth Regulation Volume: 57 Pages: 293-301
  • Peer Reviewed
• [Presentation] Analysis of DNA-binding ability and phosphorylaiton of the 2b protein of Cucumber mosaic virus (2012)
  • Author(s): Sueda, K.
  • Organizer: Gene silencing by small RNAs In Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Fairmont Hotel Vancouver(バンクーバー,カナダ)
  • Year and Date: 20120207-12
• [Presentation] Ascorbic acid accumulation is specifically induced in incompatible interaction between Brassica rapa and Turnip mosaic virus (2012)
  • Author(s): Fujiwara, A.
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Rapid and convenient diagnosis of strawberry anthracnose using a method combining macroarray and microtube hybridization (2012)
  • Author(s): 長嶋沙希
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Simultaneous detection of strawberry viruses using cDNA macroarray (2012)
  • Author(s): 古田和義
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] Virus-induced necrosis is a consequence of direct protein-protein in theraction between a viral RNA silencing suppressor and a host catalase (2012)
  • Author(s): Masuta, C.
  • Organizer: 第2回日韓合同シンポジウム
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-03-27
• [Presentation] RNA-mediated epigenetic modifications provide a tool to produce a plant that does not carry a transgene but has altered traits (2012)
  • Author(s): Kanazawa, A.
  • Organizer: Japan-Australia Symposium on Plant Science for Agriculture
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-01-19