2012 Fiscal Year Final Research Report
Creation of CYP3A- and P-glycoprotein-humanized mouse and its application to the study of drug metabolism and disposition
| Project/Area Number |
21390040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Chiba University |
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Principal Investigator |
CHIBA Kan 千葉大学, 大学院・薬学研究院, 教授 (40159033)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Kaoru 千葉大学, 大学院・薬学研究院, 准教授 (30255864)
FURIHATA Tomomi 千葉大学, 大学院・薬学研究院, 助教 (80401008)
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| Co-Investigator(Renkei-kenkyūsha) |
KAZUKI Yasuhiro 鳥取大学, 大学院・医学研究科, 助教 (90403401)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 薬物代謝 / 応用動物 |
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| Research Abstract |
CYP3A is the most important subfamily of cytochrome P450s in humans, which metabolizes around 50% of therapeutic agents. We have created humanized mouse (CYP3A-HAC mouse) which carries human CYP3A instead of mouse Cyp3a, using human artificial chromosome system, This mouse shows similar characteristics as human for the tissue- and stage-specific expression of CYP3A genes and metabolism of various therapeutic agents. Thus, this mouse is considered to be valuable for the prediction of human drug metabolism. As for P-glycoprotein- and pregnane X receptor-humanized mice, they have also been developed and their function and valuableness for the prediction of human drug metabolism are undertaken.
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Research Products
(3 results)
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[Journal Article] Trans-chromosomic mice containing a human CYP3A cluster for prediction of xenobiotic metabolism in humans2013
Author(s)
Kazuki Y, Kobayashi K, Aueviriyavit S, Oshima T, Kuroiwa Y, Tsukazaki Y, Senda N, Kawakami H, Ohtsuki S, Abe S, Takiguchi M, Hoshiya H, Kajitani N, Takehara S, Kubo K, Terasaki T, Chiba K, Tomizuka K, Oshimura M.
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Journal Title
Hum Mol Genet
Volume: 22(3)
Pages: 578-92
Peer Reviewed
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