Studies on how cytoskeleton-related molecules regulate neuronal migration, the brain formation as well as the maturation.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390052
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Fukui
• Principal Investigator: SATO Makoto 福井大学, 医学部, 教授 (10222019)
• Co-Investigator(Kenkyū-buntansha): YAGI Hideshi 兵庫医科大学, 医学部, 准教授 (10303372) ; XIE Min-jue 福井大学, 医学部, 助教 (40444210) ; IGUCHI Tokuichi 福井大学, 医学部, 特命助教 (60509305) ; IKEDA Hiroshi 福井大学, 工学研究科, 教授 (80377473)
• Project Period (FY): 2009 – 2011
• Keywords: 細胞分化 / 細胞形成 / 細胞骨格

Research Abstract

It has been demonstrated that LL5beta and FILIP bind to filamin A, one of well-known actin-binding molecules, and exert their important activities over cell migration, especially radial migration that is essential for the neocortex development. In the present study, we further pursued the possible roles of LL5beta and FILIP in the adult brain and found that LL5beta plays an important role for spine development and that FILIP for synaptic transmission through modulating the morphology of spines and subcellular localization of glutamate receptors. In addition, we observed that some actin-binding molecules, other than filamin A, are involved in their activities.

Research Products

• [Journal Article] GPR98/Gpr98 Gene Is Involved in the Regulation of Human and Mouse Bone Mineral Density (2012)
  • Author(s): Urano, T., Shiraki, M, . Yagi, H., Ito, M., Sasaki, N., Sato, M., Ouchi, Y. and Inoue, S.
  • Journal Title: J. Clin. Endocrinol. Metab Volume: 97(4) Pages: E565-74
  • DOI: doi:10.1210/jc.2011-2393
  • Peer Reviewed
• [Journal Article] A tightly controlled conditional knockdown system using the tol2 transposon-mediated technique (2012)
  • Author(s): Iguchi, T., Yagi, H., Wang, CC. and Sato, M.
  • Journal Title: PLoS ONE Volume: 7(3) Pages: e33380
  • DOI: doi:10.1371/journal.pone.0033380
  • Peer Reviewed
• [Journal Article] Maternal bisphenol A oral dosing relates to the acceleration of neurogenesis in the developing neocortex of mouse fetuses (2012)
  • Author(s): Komada, M., Asai, Y., Morii, M., Matsuki, M., Sato, M. and Nagao, T.
  • Journal Title: Toxicology Volume: 16 ; 295(1-3) Pages: 31-8
  • DOI: doi:10.1016/j.tox.2012.02.0132012
  • Peer Reviewed
• [Journal Article] LL5βdirects the translocation of Filamin A and SHIP2 to sites of PtdIns(3, 4, 5) P3 accumulation and PtdIns(3, 4, 5) P3 localization is mutually modified by co-recruited SHIP2 (2010)
  • Author(s): Takabayashi, T., Xie, M.-J., Takeuchi, S., Kawasaki, M., Yagi, H., Okamoto, M., Tariqur, R. M., Malik, F., Kuroda, K., Kubota, C., Fujieda, S., Nagano, T. and Sato, M.
  • Journal Title: J. Biol. Chem Volume: 285(21) Pages: 16155-16165
  • DOI: doi:10.1074/jbc.M109.081901
  • Peer Reviewed
• [Journal Article] Inhibition of calpain increases LIS1 expression and partially rescues in vivo phenotypes in a mouse model of lissencephaly (2009)
  • Author(s): Yamada, M., Yoshida, Y., Mori, D., Takitoh, T., Kengaku, M., Umeshima, H., Takao, K., Miyakawa, T., Sato, M., Sorimachi, H., Wynshaw-Boris, A. and Hirotsune, S.
  • Journal Title: Nat. Med Volume: 15(10) Pages: 1202-1207
  • DOI: doi:10.1038/nm.2023
  • Peer Reviewed
• [Journal Article] Deficiency of Vlgr1 resulted in deafness and susceptibility to audiogenic seizures while the degree of hearing impairment was not correlated with seizure severity in C57BL/6-and 129-backcrossed lines of Vlgr1 knockout mice (2009)
  • Author(s): Yagi, H., Noguchi, Y., Kitamura, K. and Sato, M.
  • Journal Title: Neurosci Lett Volume: 18 ; 461(2) Pages: 190-195
  • URL: http://dx.doi.org/10.1016/j.neulet
• [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration
  • Author(s): Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M.
  • Journal Title: Cerebral Cortex Volume: (in press)
  • DOI: doi:10.1093/cercor/bhs123
  • Peer Reviewed
• [Presentation] 大脳皮質形成とスパイン形成の新たな共通分子基盤とその展開.シンポジウム(S19-3)「神経発生・形成における細胞骨格の意義」 (2012)
  • Author(s): 佐藤真
  • Organizer: 第117回日本解剖学会全国学術集会
  • Place of Presentation: 甲府市
  • Year and Date: 2012-03-28
• [Presentation] Molecular aspects shared by the cortical development and the spine formation, and their potential involvement in the network remodeling (2011)
  • Author(s): 佐藤真
  • Organizer: AACL-2011(Asian Aging Core for Longevity)長崎シンポジウムJapan-Korea Joint Conference on Brain Aging and Neurodegeneration
  • Place of Presentation: 長崎
  • Year and Date: 2011-11-21
• [Presentation] Dendritic growth cone activities, regulated by Abl kinase and Cdk5 via WAVE2-Abi2, are essential for completing multipolar-bipolar transition and starting glia-guided locomotion (2011)
  • Author(s): Xie, M.-J., et al.
  • Organizer: Society For Neuroscience 2011
  • Place of Presentation: ワシントン
  • Year and Date: 2011-11-15
• [Presentation] dendritic growth cone activity regulated by Abl kinase and Cdk5 via WAVE2-Abi2 is essential for completing the multipolar-bipolar transition and initiating glia-guided locomotion. (2011)
  • Author(s): 佐藤真
  • Organizer: シンポジウム(S06-2)「神経形態制御から機能へ-スペーシャルセルバイオロジーの観点から」第54回日本神経化学会大会
  • Place of Presentation: 石川県山代
  • Year and Date: 2011-09-27
• [Presentation] 破綻から学ぶ脳形成・発達のしくみ:細胞分化・遊走から代謝、精神疾患まで (2010)
  • Author(s): 佐藤真
  • Organizer: 特別講演,日本薬学会北陸支部特別講演会
  • Place of Presentation: 富山
  • Year and Date: 2010-06-09
• [Presentation] 大脳皮質において棘形成・発達と神経細胞遊走を司る新たな分子機構(S1-3)神経の発生・再生・移植研究会 (2010)
  • Author(s): 佐藤真
  • Organizer: 第25回学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-22
• [Presentation] Phosphatidylinositol(3, 4, 5)-triphosphate associated factor LL5βregulates morphology and maturation of dendritic spines (2009)
  • Author(s): Xie, M-J., Yagi, H., Iguchi, T. and Sato, M
  • Organizer: ポスター大阪大学グローバルCOEポログラム"System Dynamics of Biological Fuction"
  • Place of Presentation: 淡路島
  • Year and Date: 2009-10-09
• [Book] iPS細胞研究とその周辺技術、「特集1神経系におけるiPS細胞(iPS細胞の活用も含めた神経機能修復の現状と将来) (2011)
  • Author(s): 黒田一樹,佐藤真
  • Total Pages: 30(234)-34(238)
  • Publisher: 金芳堂
• [Remarks]
  • URL: http://www.med.u-fukui.ac.jp/KAIBOU2/