2011 Fiscal Year Final Research Report
Structure of recombinant habutobin protein and relation withexpression of antithrombotic activity
| Project/Area Number |
21390060
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Mariko 琉球大学, 大学院・医学研究科, 准教授 (40180400)
SUNAGAWA Masanori 琉球大学, 大学院・医学研究科, 助教 (70325835)
UEDA Tomoyuki 琉球大学, 医学部, 准教授 (80160175)
EGUCHI Yukinori 琉球大学, 医学部, 准教授 (50160354)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 抗血栓活性 / 抗血小板剤 / 血小板 / ハブトビン / 組換え蛋白体 / 蛇毒 |
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| Research Abstract |
Habutobin expresses antithrombotic action by defibrinogenation, acceleration of fibrinolysis, and inhibition of platelet aggregation. Structural determination of the site responsible for the action of habutobin underlies the development of antithrombotic agents with multiple pharmacological actions. Habutobin cDNA was cloned and 4 fragments of recombinant habutobin (habu-mut 1, 2, 3 and 4) each with different target sites were constructed. It was found that habu-mut2 suppressed collagen-induced platelet aggregation by approximately 10%, and habu-mut3 suppressed it by approximately 20-30%. Novel antithrombotic agents can be developed by structural analysis.
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