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2011 Fiscal Year Final Research Report

Identification of endogenous SID-1 ligands

Research Project

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Project/Area Number 21390063
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionTokyo Women's Medical University

Principal Investigator

MITANI Shohei  東京女子医科大学, 医学部, 教授 (90192757)

Project Period (FY) 2009 – 2011
Keywords分子 / 細胞生理学
Research Abstract

RNAi(RNA interference) is now a popular method to downregulate gene functions of interest. In C. elegans, it has been known that dsRNAs transmit from cells to other cells. This phenomenon is called as systemic RNAi and SID-1 protein is the representative gene that is required for dsRNA uptake in systemic RNAi. We searched for molecules involved in the phenomenon and found that animals are resistant to gon-1 RNAi in the vps-45 mutant background. GON-1 protein is a metalloprotease and necessary for secretion of various proteins from distal tip cells and body wall muscle cells. We found that GON domain alone is important for this secretion.

  • Research Products

    (32 results)

All 2012 2011 2010 2009 Other

All Journal Article (31 results) Remarks (1 results)

  • [Journal Article] The Caenorhabditis elegans HEN1 ortholog, HENN-1, methylates and stabilizes select subclasses of germline small RNAs2012

    • Author(s)
      Billi AC, Alessi AF, Khivansara V, Han T, Freeberg M, Mitani S and Kim JK
    • Journal Title

      PLoS Genetics

      Volume: 8(4) Pages: e102617

  • [Journal Article] GPI anchor synthesis in the distal tip cells is indispensable for development and differentiation of germline cells of the nematode Caenorhabditis elegans2012

    • Author(s)
      Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y, Ito S, Gengyo-Ando K, Kage-Nakadai E, Mitani S and Nomura K
    • Journal Title

      Mol. Biol. Cell

      Volume: 23(6) Pages: 982-995

  • [Journal Article] Phosphorylation of α-synuclein at Ser129 reduces neuronal dysfunction by lowering its membrane-binding property in Caenorhabditis elegans2012

    • Author(s)
      Kuwahara T, Tonegawa R, Mitani S, Iwatsubo T
    • Journal Title

      J Biol Chem.

      Volume: 287(10) Pages: 7098-109

  • [Journal Article] LYCAT, a homologue of C. elegans acl-8, acl-9 and acl-10, determines the fatty acid composition of phosphatidylinositol in mice2012

    • Author(s)
      Imae R, Inoue T, Nakasaki Y, Uchida Y, Ohba Y, Mitani S and Arai H
    • Journal Title

      J. Lipid Res.

      Volume: 53(3) Pages: 335-47

  • [Journal Article] Single/Low-copy integration of transgenes in Canehorhabditis elegans by an ultraviolet and trimethylpsolaren method2012

    • Author(s)
      Kage-Nakadai E, Kobuna H, Funatsu O, Otori M, Gengyo-Ando K, Yoshina S, Hori S and Mitani S
    • Journal Title

      BMC Biotechnology

      Volume: 12(1) Pages: 1-9

  • [Journal Article] Expression of ATP sensor protein in Caenorhabditis elegans2012

    • Author(s)
      Kishikawa J, Fujikawa M, Imamura H, Yasuda K, Noji H, Ishii N, Mitani S, and Yokoyama K
    • Journal Title

      Microscopy Research and Technique

      Volume: 75(1) Pages: 15-19

  • [Journal Article] Osmotic shock results in hmit induction in C. elegans glial and excretory canal cells2011

    • Author(s)
      Kage-Nakadai E, Uehara T and Mitani S
    • Journal Title

      Biochem. Biophys. Res. Comm

      Volume: 410 Pages: 471-477

  • [Journal Article] Ceramide glucosyltransferease of the nematode Caenorhabditis elegans is involved in oocyte maturation and in early embryonic cell division2011

    • Author(s)
      Nomura K, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M and Nomura K
    • Journal Title

      Glycobiology

      Volume: 21 Pages: 834-848

  • [Journal Article] Intracellular PLA1 and acyltransferase involved in C. elegans stem cell divisions determine the sn-1 fatty acyl chain of phosphatidylinositol2010

    • Author(s)
      Imae R, Inoue T, Kimura M, Kanamori T, Hara N, Kage-Nakadai E, Mitani S, and Arai H
    • Journal Title

      Mol. Biol. Cell

      Volume: 21 Pages: 3114-3124

  • [Journal Article] Two Golgi-resident 3'-phosphoadenosine 5'-phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans2010

    • Author(s)
      Dejima K, Murata D, Mizuguchi S, Nomura KH, Izumikawa T, Kitagawa H, Gengyo-Ando K, Yoshina S, Ichimiya T, Nishihara S, Mitani S and Nomura K
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 24717-24728

  • [Journal Article] Multivesicular Body Formation Requires OSBP. Related Proteins and Cholesterol2010

    • Author(s)
      Kobuna H, Inoue T, Shibata M, Gengyo-Ando K, Yamamoto K, Mitani S, Arai H
    • Journal Title

      PLoS Genetics

      Volume: 6(8) Pages: e1001055

  • [Journal Article] The Arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans2010

    • Author(s)
      Nakae I, Fujino T, Kobayashi T, Sasaki A, Kikko Y, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T
    • Journal Title

      Mol. Biol. Cell

      Volume: 21 Pages: 2434-2442

  • [Journal Article] EAK-7 controls development and lifespan by regulating nuclear DAF-16/FoxO activity2010

    • Author(s)
      Alam H, Williams T, Yoshina S, Mitani S, and Hu PJ
    • Journal Title

      Cell Metabolism

      Volume: 12 Pages: 30-41

  • [Journal Article] C. elegans transthyretin-like protein TTR-52 mediates recognition of apoptotic cells by CED-1 phagocyte receptor2010

    • Author(s)
      Wang X, Li W, Zhao D, Liu B, Shi Y, Chen B, Yang H, Guo P, Geng X, Shang Z, Peden E, Kage-Nakadai E, Mitani S, and Xue D
    • Journal Title

      Nature Cell Biol

      Volume: 12 Pages: 655-664

  • [Journal Article] An Arf-like small G protein, ARL-8, Promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation2010

    • Author(s)
      Klassen MP, Wu YE, Maeder CI, Nakae I, Cueva JG, Lehrman EK, Tada M, Gengyo-Ando K, Wang GJ, Goodman M, Mitani S, Kontani K, Katada T and Shen K
    • Journal Title

      Neuron

      Volume: 66 Pages: 710-723

  • [Journal Article] Protein phosphatase 2A cooperates with the autophagy-related kinase UNC-51 to regulate axon guidance in Caenorhabditis elegans2010

    • Author(s)
      Ogura K, Mitani S, Gengyo-Ando K, Baillie DL, Kohara Y and Goshima Y
    • Journal Title

      Development

      Volume: 137 Pages: 1657-1667

  • [Journal Article] Caspase-dependent conversion of Dicer ribonuclease into a death-promoting deoxyribonuclease2010

    • Author(s)
      Nakagawa A, Shi Y, Kage-Nakadai, Mitani S and Xue D
    • Journal Title

      Science

      Volume: 328 Pages: 327-334

  • [Journal Article] Combinatorial regulation of three TRPM channels governs magnesium homeostasis2010

    • Author(s)
      Teramoto T, Sternick LA, Kage-Nakadai E, Sajjadi S, Siembida J, Mitani S, Iwasaki K and Lambie EJ
    • Journal Title

      PLoS One

      Volume: 5(3) Pages: e9589

  • [Journal Article] Two Very Long Chain Fatty Acid Acyl-CoA Synthetase Genes, acs-20 and acs-22, Have Roles in the Cuticle Surface Barrier in Caenorhabditis elegans2010

    • Author(s)
      Kage-Nakadai E, Kobuna H, Kimura M, Gengyo-Ando K, Inoue T, Arai H, Mitani S
    • Journal Title

      PLoS One

      Volume: 5(1) Pages: e8857

  • [Journal Article] NBRP databases : Databases of biological resources in Japan2010

    • Author(s)
      Yamazaki Y, Akashi R, Banno Y, Endo T, Ezura H, Fukami-Kobayashi K, Inaba K, Isa T, Kamei K, Kasai F, Kobayashi M, Kurata N, Kusaba M, Matuzawa T, Mitani S, Nakamura T, Nakamura Y, Nakatsuji N, Naruse K, Niki H, Nitasaka E, Obata Y, Okamoto H, Okuma M, Sato K, Serikawa T, Shiroishi T, Sugawara H, Urushibara H, Yamamoto M, Yaoita Y, Yoshiki A, and Kohara Y
    • Journal Title

      Nucl. Acid Res

      Volume: 38 Pages: D26-D32

  • [Journal Article] Distinct Argonaute-mediated 22G-RNA pathways direct genome surveillance in the C. elegans germline2009

    • Author(s)
      Gu W, Shirayama M, Conte D Jr, Vasale JJ, Batista PJ, Claycomb JM, Keys J, Chen CG, Chaves DA, Duan SE, Kasschau KD, Falgren N, Mitani S, Carrington JC, Mello CC
    • Journal Title

      Mol. Cell

      Volume: 36 Pages: 231-244

  • [Journal Article] The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation2009

    • Author(s)
      Claycomb JM, Batista PJ, Pang KM, Gu W, 1 Vasale JJ, van Wolfswinkel JC, Chaves DA, Shirayama M, Mitani S, Ketting RF, Conte D, Jr., and Mello CC
    • Journal Title

      Cell

      Volume: 139 Pages: 123-134

  • [Journal Article] Differential roles of Caenorhabditis elegans DNase II genes in apoptotic DNA degradation and development2009

    • Author(s)
      Lai H-J, Lo SJ, Kage-Nakadai E, Mitani S, Xue D
    • Journal Title

      PLoS One

      Volume: 4 Pages: 1-13

  • [Journal Article] Biological function of the C. elegans glycosphingolipid-binding galectin LEC-82009

    • Author(s)
      Ideo H, Fukushima K, Gengyo-Ando K, Mitani S, Dejima K, Nomura K & Yamashita K
    • Journal Title

      J. Biol. Chem

      Volume: 284 Pages: 26493-501

  • [Journal Article] FLR-2, the glycoprotein hormone alpha 2 subunit, is involved in the neural control of intestinal functions in Caenorhabditis elegans2009

    • Author(s)
      Oishi A, Gengyo-Ando K, Mitani S, Mohri-Shiomi A, Kimura KD, Ishihara T and Katsura I
    • Journal Title

      Genes to Cells

      Volume: 14 Pages: 1141-54

  • [Journal Article] C. elegans caspase homolog CSP-2 inhibits CED-3 autoactivation and apoptosis in germ cells2009

    • Author(s)
      Geng X, Zhou Q, Kage-Nakadai E, Shi, Yan N, Mitani S and Xue D
    • Journal Title

      Cell Death and Differentiation

      Volume: 16 Pages: 1385-94

  • [Journal Article] Functional analysis of GS28, an intra-Golgi v-SNARE2009

    • Author(s)
      Maekawa M, Inoue T, Kobuna H, Nishimura T, Gengyo-Ando K, Mitani S and Arai H
    • Journal Title

      C. elegans Genes to Cells

      Volume: 14 Pages: 1003-13

  • [Journal Article] The functional homolog of the human nucleotide-sugar transporter like protein(hUGTrel1) is involved in maintenance of ER homeostasis and essential for larval development in the nematode Caenorhabditis elegans2009

    • Author(s)
      Dejima K, Murata D, Mizuguchi S, Nomura KH, Gengyo-Ando K, Mitani S, Kamiyama S, Nishihara S, and Nomura K
    • Journal Title

      FASEB J

      Volume: 23 Pages: 2215-2225

  • [Journal Article] Normal formation of Caenorhabditis elegans intestinal granules requires ABC transporters HAF-4 and HAF-9, which are highly homologous to human lysosomal peptide transporter TAPL(TAP-like)2009

    • Author(s)
      Kawai H, Tanji T, Shiraishi H, Yamada M, Iijima R, Inoue T, Kezuka Y, Ohashi K, Yoshida Y, Tohyama K, Gengyo-Ando K, Mitani S, Arai H, Ohashi-Kobayashi A, and Maeda M
    • Journal Title

      Mol. Biol. Cell

      Volume: 20 Pages: 2797-2990

  • [Journal Article] Identification of a novel ADAMTS9/GON-1 function for protein transport from the ER to the Golgi

    • Author(s)
      Yoshina S, Sakaki K, Yonezumi-Hayashi A, Gengyo-Ando K, Inoue H, Iino Y & Mitani S
    • Journal Title

      Mol. Biol. Cell

      Volume: (in press)

  • [Journal Article] Reduced Expression of BTBD10, an Akt activator, Drives Impairment of Motor Function

    • Author(s)
      Nawa M, Kage-Nakadai E, Aiso S, Okamoto K, Mitani S, and Matsuoka M
    • Journal Title

      Cell Death and Differentiation

      Volume: (in press)

  • [Remarks]

    • URL

      http://www.twmu.ac.jp/Basic/physiol2/

URL: 

Published: 2013-07-31  

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