Analysis of genes encoding secretory and membrane protein by CAST method and microRNA regulation in esophago-gastric cancer and its clinical application

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390105
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Hiroshima University
• Principal Investigator: YASUI Wataru 広島大学, 大学院・医歯薬学総合研究科, 教授 (40191118)
• Project Period (FY): 2009 – 2011
• Keywords: 胃癌 / 食道扁平上皮癌 / 膜蛋白・分泌蛋白 / CAST法 / microRNA / 診断・治療

Research Abstract

CAST method was carried out on esophago-gastric cancers and identified DSC2, TSPAN8, TM9SF3, etc. as possible therapeutic target. By microRNA microarray, several microRNAs were identified as diagnostic marker. miR-148a and miR-143 were found to participate in invasion and morphogenesis. New development of diagnosis and therapeutics using novel markers is expected

Research Products

• [Journal Article] Upregulation of HOXAio in gastric cancer with the intestinal mucin phenotype : Reduction during tumor progression and favorable prognosis (2012)
  • Author(s): Sentani K, one N, Naito Y, Sakamoto N, Anami K, 00 HZ, Uraoka N, Aoyagi K, Sasaki H and Yasui W
  • Journal Title: Carcinogenesis Volume: (in press)
  • Peer Reviewed
• [Journal Article] Cytokeratin 7 is a predictive marker for survival and effect of adjuvant chemotherapy in patients with esophageal squamous cell carcinoma (2012)
  • Author(s): oue N, Noguchi T, Anami K, Kitano S, Sakamoto N, Sentani K, Uraoka N, Aoyagi K, Yoshida T, Sasaki H and Yasui W
  • Journal Title: Ann Surg Oncol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Deficiency of claudin-l8 causes paracellular H+ leakage, up regulation of interleukinlbeta, and atrophic gastritis in mice (2012)
  • Author(s): Hayashi D, Tamura A, Tanaka H, Yamazaki Y, Watanabe S, Suzuki K, Suzuki K, Sentani K, Yasui W, Rakugi H, Isaka Y and Tsukita S
  • Journal Title: Gastroenterology Volume: 142 Pages: 292-304
  • Peer Reviewed
• [Journal Article] Identification of transmembrane protein in prostate cancer by the Escherichia coll ampicillin secretion trap : Expression of CDON is involved in tumor cell growth and invasion (2011)
  • Author(s): Hayashi T, one N, Sakamoto N, Anami K, 00 HZ, Sentani K, Ohara S, Teishima J, Matsubara A and Yasui W
  • Volume: 78
  • Pages: 277-284
  • Peer Reviewed
• [Journal Article] Antistromal therapy with imatinib inhibits growth and metastasis of gastric carcinoma in an orthotopic mouse model (2011)
  • Author(s): Sumida T, Kitadai Y, Shinagawa K, Tanaka M, Kodama M, Ohnishi M, Ohara E, Tanaka S, Yasui W and Chayama K
  • Volume: 128
  • Pages: 2050-2062
  • Peer Reviewed
• [Journal Article] A functional SNP in MUCl, at chromosome lq22, determines susceptibility to diffuse-type gastric cancer Short title : MUClis a gastric cancer susceptibility gene (2011)
  • Author(s): Saeki N, Saito A, Choi II, Matsuo K, Ohnami S, Totsuka H, Chiku S, Kuchiba A, Lee YS, Yoon KA, Kook MC, Park SR, Kim YW, Tanaka H, Ta j ima K, Hirose H, Tanioka F, Matsuno Y, Sugimura H, Kate S, Nakamura T, Nishina T, Yasui W, Aoyagi K, Sasaki H, Yanagiha
  • Journal Title: Gastroenterology Volume: 140 Pages: 892-902
  • Peer Reviewed
• [Journal Article] Upregulation of connexin 30 in intestinal phenotype gastric cancer and its reduction during tumor progression (2010)
  • Author(s): Sentani K, one N, Sakamoto N, Anami K, Naito Y, Aoyagi K, Sasaki H and Yasui W
  • Journal Title: Pathobiology Volume: 77 Pages: 241-248
  • Peer Reviewed
• [Journal Article] Chemoprevention by nonsteroidal antiinflammatory drug eliminates oncogenic intestinal stem cells via SMACdependent apoptosis (2010)
  • Author(s): Qui W, Wang X, Leibowitz, Lin H, Barker N, Okada H, one N, Yasui W, Clevers H, Schoen RE, Yu J and Zhang L
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 20027-20032
  • Peer Reviewed
• [Journal Article] CDX2 regulates multidrug resistance 1 gene expression in malignant intestinal epithelium (2010)
  • Author(s): Takakura Y, Hinoi T, one N, Sasada T, Kawaguchi Y, Okajima M, Akyol A, Fearon ER, Yasui W and Ohdan H
  • Journal Title: Cancer Res Volume: 70 Pages: 6767-6778
  • Peer Reviewed
• [Journal Article] Potential role for vascular endothelial growth factor-D as an autocrine factor for human gastric carcinoma cells (2010)
  • Author(s): Tanaka M, Kitadai Y, Kodama M, Shinagawa K, Sumida T, Tanaka S, one N, Yasui W and Chayama K
  • Journal Title: Cancer Sci Volume: 101 Pages: 2121-2127
  • Peer Reviewed
• [Journal Article] Expression of plateletderived growth factor(PDGF)-B and PDGFreceptor b is associated with lymphatic metastasis in human gastric carcinoma (2010)
  • Author(s): Kodama M, Kitadai Y, Sumida T, Ohnishi M, Ohara E, Tanaka M, Shinagawa K, Tanaka S, Yasui W and Chayama K
  • Journal Title: Cancer Sci Volume: 101 Pages: 1984-1989
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells enhance growth and metastasis of colon cancer (2010)
  • Author(s): Shinagawa K, Kitadai Y, Tanaka M, Sumida T, Kodama M, Higashi Y, Tanaka S, Yasui W and Chayama K
  • Journal Title: Int J Cancer Volume: 127 Pages: 2323-2333
  • Peer Reviewed
• [Journal Article] Search for transmembrane protein in gastric cancer by the Escherichia coll ampicillin secretion trap : expression of DSC2 in gastric cancer with intestinal phenotype (2010)
  • Author(s): Anami K, one N, Noguchi T, Sakamoto N, Sentani K, Hayashi T, Hinoi T, Okajima M, Graft IM and Yasui W
  • Journal Title: J Pathol Volume: 221 Pages: 275-284
  • Peer Reviewed
• [Journal Article] Relation between microRNA expression and progression and prognosis of gastric cancer : a microRNA expression analysis (2010)
  • Author(s): Ueda T, Volinia S, Okumura H, Shimizu M, Taccioli C, Rossi S, Alder H, Lin CG, one N, Yasui W, Yoshida K, Sasaki H, Nomura S, Seto Y, Kaminishi M, Carin GA and Croce CM
  • Journal Title: Lancet Oncol Volume: 11 Pages: 136-146
  • Peer Reviewed
• [Journal Article] Serial analysis of gene expression of esophageal squamous cell carcinoma : ADAMTSl6 is upregulated in esophageal squamous cell carcinoma (2010)
  • Author(s): Sakamoto N, one N, Noguchi T, Sentani K, Anami K, Sanada Y, Yoshida K and Yasui W
  • Journal Title: Cancer Sci Volume: 101 Pages: 1038-1044
  • Peer Reviewed
• [Journal Article] Wntsa signaling is involved in aggressiveness of prostate cancer and expression of metalloproteinase (2010)
  • Author(s): Yamamoto H, one N, Sate A, Hasegawa Y, Yamamoto H, Matsubara A, Yasui W and Kikuchi A
  • Journal Title: Oncogene Volume: 29 Pages: 2036-2046
  • Peer Reviewed
• [Journal Article] Transcriptome dissection of gastric cancerIdentification of novel diagnostic and therapeutic targets from pathology specimens(review article) (2009)
  • Author(s): Yasui W, one N, Sentani K, Sakamoto N and Motoshita J
  • Journal Title: Pathol Int cancer Volume: 59 Pages: 121-136
  • Peer Reviewed
• [Journal Article] Serum olfactomedin 4(GWll2, hGC-l) in combination with Reg IV is a highly sensitive biomarker for gastric cancer patients (2009)
  • Author(s): Oue N, Sentani K, NOGUCHI T, Ohara S, Sakamoto N, Hayashi T, Anami K, Motoshita J, Ito M, Tanaka S, Yoshida K and Yasui W
  • Journal Title: Int J Cancer Volume: 125 Pages: 2382-2392
  • Peer Reviewed
• [Journal Article] Laminin gamma2 mediates Wnt5a-induced invasion by gastric cancer cells (2009)
  • Author(s): Yamamoto H, Kitadai Y, Yamamoto H, one N, Ohdan H, Yasui W and Kikuchi A
  • Journal Title: Gastroenterol Volume: 137 Pages: 242-252
  • Peer Reviewed
• [Journal Article] h-prune is an independent prognostic marker for survival in esophageal squamous cell carcinoma (2009)
  • Author(s): Noguchi T, one N, Wada S, Sentani K, Sakamoto N, Kikuchi A and Yasui W
  • Journal Title: Ann Surg Oncol Volume: 16 Pages: 1390-1396
  • Peer Reviewed
• [Journal Article] Characteristic gene expression in stromal cells of gastric cancers among atomic-bomb survivors (2009)
  • Author(s): one N, Sentani K, Sakamoto N, Motoshita J, Nishisaka T, Fukuhara T, Matsuura H, Sasaki H, Nakachi K and Yasui W
  • Journal Title: Int J Cancer Volume: 124 Pages: 1112-1121
  • Peer Reviewed
• [Presentation] CAST法で同定したTSPAN8の胃癌における発現と機能解析 (2011)
  • Author(s): 阿南勝宏、大上直秀、坂本直也、仙谷和弘、浦岡直礼、内藤寛、HtooZarniOo、野口剛、安井弥
  • Organizer: 第22回日本消化器癌発生学会、ワークショップ2
  • Place of Presentation: 佐賀
  • Year and Date: 20111125-26
• [Presentation] 胃癌におけるmiR-148aの発現低下とMMP7を介した浸潤への関与 (2011)
  • Author(s): 坂本直也、内藤寛、仙谷和弘、大上直秀、安井弥
  • Organizer: 第22回日本消化器癌発生学会、ワークショップ3
  • Place of Presentation: 佐賀
  • Year and Date: 20111125-26
• [Presentation] CAST法を用いた胃癌細胞株における遺伝子発現解析 (2011)
  • Author(s): 阿南勝宏、大上直秀、坂本直也、林哲太郎、仙谷和弘、浦岡直礼、HtooZarniOo、内藤寛、坂根潤一、後藤景介、野口剛、安井弥
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111003-05
• [Presentation] SAGE法を用いた食道扁平上皮癌の解析:食道扁平上皮癌におけるNRD1の発現亢進 (2011)
  • Author(s): 浦岡直礼、坂本直也、大上直秀、仙谷和弘、阿南勝宏、野口剛、真田雄市、吉田和弘、安井弥
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111003-05
• [Presentation] Gene expression profile of scirrhous type gastric cancer analyzed by CAST method (2011)
  • Author(s): Oo HZ, Uraoka N, Hayashi T, Anami K, Sakamoto N, Sentani K, Oue N and Yasui W
  • Organizer: 8th International Symposium on Minimal Residual Cancer
  • Place of Presentation: Osaka (Japan)
  • Year and Date: 20110921-23
• [Presentation] Identification of novel diagnostic and therapeutic targets of gastric cancer by transcriptome dissection (2011)
  • Author(s): Yasui W
  • Organizer: The Third CREAST-SBM International Conference
  • Place of Presentation: Hiroshima (Japan)
  • Year and Date: 20110608-09
• [Presentation] 胃癌の発生・進展の分子機序のマイルストーン (2011)
  • Author(s): 安井弥
  • Organizer: 第100回日本病理学会総会、シンポジウム1
  • Place of Presentation: 横浜
  • Year and Date: 20110428-30
• [Presentation] 胃癌におけるmicroRNA発現解析 (2011)
  • Author(s): 坂本直也、内藤寛、仙谷和弘、大上直秀、安井弥
  • Organizer: 第100回日本病理学会総会、ワークショップ1
  • Place of Presentation: 横浜
  • Year and Date: 20110428-30
• [Presentation] SAGE法を用いた食道扁平上皮癌の新規マーカーNRD1の同定 (2011)
  • Author(s): 浦岡直礼、坂本直也、大上直秀、仙谷和弘、阿南勝宏、野口剛、真田雄市、吉田和弘、安井弥
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20110428-30
• [Presentation] Novel diagnostic and therapeutic targets of gastric cancer identified by" Omics" study (2011)
  • Author(s): YasuiW, SentaniK, SakamotoN, AnamiK, NaitoY and OueN
  • Organizer: 9^<th> International Gastric Cancer
  • Place of Presentation: Seoul(Korea)
  • Year and Date: 20110420-23
• [Presentation] Gene expression profile in scirrhous type gastric mcancer by CAST method. 9th International Gastric Cancer Congress (2011)
  • Author(s): OoHZ, UraokaN, HayashiT, AnamiK, SakamotoN, SentaniK, OueN and YasuiW
  • Organizer: 9^<th> International Gastric Cancer Congress, Seoul(Korea)
  • Place of Presentation: Seoul(Korea)
  • Year and Date: 20110420-23
• [Presentation] Unique microRNA signatures associated with progression and prognosis of gastric cancer. 9th International Gastric Cancer Congress (2011)
  • Author(s): UedaT, YasuiW, YoshidaK, SasakiS, NomuraS, SetoY, KaminishiM, CalinG, CroceC, VoliniaS, OkumuraH, ShimizuM, TaccioliC, RossiS, AlderH, LiuC and OueN
  • Organizer: 9^<th> International Gastric Cancer Congress, Seoul(Korea)
  • Place of Presentation: Seoul(Korea)
  • Year and Date: 20110420-23
• [Presentation] miR-l48aa is down羊egulated in gastric cancer and involved in gastric cancer invasion by repression ofMMPi7 expression (2011)
  • Author(s): SakamotoN, NaitoY, UraokaN, AnamiK, SentaniK, OueN and YasuiW
  • Organizer: 9^<th> International Gastric Cancer Congress, Seoul(Korea)
  • Place of Presentation: Seoul(Korea)
  • Year and Date: 20110420-23
• [Presentation] Molecular character of gastric and intestinal phenotypes of gastric cancer (2011)
  • Author(s): YasuiW, OueN, SentaniK, and SakamotoN
  • Organizer: The 6th International Conference on Gastroenterological Carcinogenesis, Lecture
  • Place of Presentation: Houston, Texas(USA)
  • Year and Date: 20110106-08
• [Presentation] トランスクリプトーム解析による新規診断標的の同定とがん病理診断への応用 (2010)
  • Author(s): 安井弥
  • Organizer: 第69回日本癌学会学術総会、シンポジウム5
  • Place of Presentation: 大阪
  • Year and Date: 20100922-24
• [Presentation] 胃癌の進行と予後に関与するmicroRNAの探索 (2010)
  • Author(s): 上田哲也、StefanoVolinia、奥村浩、Chang-goneLiu、大上直秀、安井弥、吉田和弘、佐々木博己、野村幸世、瀬戸泰之、上西紀夫、GeorgeA. Calin、CarloM. Croce
  • Organizer: 第69回日本癌学会学術総会、インターナショナルセッション8
  • Place of Presentation: 大阪
  • Year and Date: 20100922-24
• [Presentation] 胃癌における細胞表面蛋白質desmocollin2の解析 (2010)
  • Author(s): 大上直秀、阿南勝宏、内藤寛、坂本直也、仙谷和弘、安井弥
  • Organizer: 第19回日本がん転移学会学術集会・総会ミニシンポジウム(2)
  • Place of Presentation: 金沢
  • Year and Date: 20100616-17
• [Presentation] Desmocollin 2(DSC2), identified by CAST method, is associated with intestinal phenotype of gastric cancer (2010)
  • Author(s): AnamiK, OueN, SakamotoN, HayashiT, OharaS, SentaniK, NoguchiT and Yasui W
  • Organizer: The 101st Annual Meeting of the American Association for Cancer Research
  • Place of Presentation: Maryland(USA)
  • Year and Date: 20100417-21
• [Presentation] Serial Analysis of gene expression(SAGE) of esophageal squamous cell carcinoma : ADAMTS-l66 is up-regulated in esophageal squamous cell carcinoma (2010)
  • Author(s): SakamotoN, OueN, NoguchiT, SentaniK, AnamiK, SanadaY, YoshidaK and YasuiW
  • Organizer: The 101st Annual Meeting of the American Association for Cancer Research, Washington D. C.
  • Place of Presentation: Maryland(USA)
  • Year and Date: 20100417-21
• [Presentation] Molecular basis of intestinal and gastric phenotypes of gastric cancer (2010)
  • Author(s): YasuiW
  • Organizer: The 17th Seoul International Cancer Symposium" Gastric Cancer Update 2010"
  • Place of Presentation: Seoul(Korea)
  • Year and Date: 2010-06-01
• [Presentation] 胃がんの新規診断治療標的の同定と展開 (2009)
  • Author(s): 安井弥
  • Organizer: 第68回日本癌学会学術総会、シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 20091001-03
• [Presentation] 胃がんの組織像とオーミクス (2009)
  • Author(s): 安井弥
  • Organizer: 第6回日本病理学会カンファレンス、レクチャー
  • Place of Presentation: 筑波
  • Year and Date: 20090731-0801
• [Presentation] Identification of potential therapeutic targets in gastric cancer by basic and translational research state of art lecture- (2009)
  • Author(s): YasuiW
  • Organizer: 8^<th> International Gastric Cancer Congress, Lectureat Plenary session 1
  • Place of Presentation: Krakow(Poland)
  • Year and Date: 20090610-13
• [Book] Histoogical and serological tumor markers of gastric cancer. In Histological and serological tumor markers and their clinical usefulness in cancers, ed. By Dan Hellberg (2009)
  • Author(s): YasuiW, SentaniK, SakamotoN, MotoshitaJandOueN
  • Total Pages: 93-111
  • Publisher: Nova Science Publishers, NewYork
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/byori1/