Study on roles of basophils under physiological and pathological conditions

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390116
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KARASUYAMA Hajime 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (60195013)
• Project Period (FY): 2009 – 2011
• Keywords: 炎症 / 好塩基球 / アレルギー / 寄生虫感染 / モデルマウス

Research Abstract

The functional analysis of basophils in vivo has long been hampered by the lack of model animals that are deficient only in basophils. To overcome this limitation, we established Mcpt8^<DTR> mice in that basophils can be conditionally ablated by administration of diphtheria toxin. Taking advantage of this mouse model, we showed that basophils play an important role in the acquired protective immunity against tick infestation.

Research Products

• [Journal Article] Th2-inducing cytokines IL-4 and IL-33 synergistically elicit the expression of transmembrane TNF-αon macrophages through the autocrine action of IL-6 (2012)
  • Author(s): Ogawa, H., Mukai, K., Kawano, Y., Minegishi, Y., and Karasuyama, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 420 Pages: 114-118
  • Peer Reviewed
• [Journal Article] Role of mast cells and basophils in IgE responses and in allergic airway hyperresponsiveness (2012)
  • Author(s): Sawaguchi, M., Tanaka, S., Nakatani, Y., Harada, Y., Mukai, K., Matsunaga, Y., Ishiwata, K., Oboki, K., Kambayashi, T., Watanabe, N., Karasuyama, H., Nakae, S., Inoue, H., and Kubo, M.
  • Journal Title: J. Immunol Volume: 188 Pages: 1809-1818
  • Peer Reviewed
• [Journal Article] Nonredundant roles of basophils in immunity (2011)
  • Author(s): Karasuyama, H., Mukai, K., Obata, K., Tsujimura, Y., and Wada, T.
  • Journal Title: Annu. Rev. Immunol Volume: 29 Pages: 45-69
  • Peer Reviewed
• [Journal Article] Mouse and human neutrophils induce anaphylaxis (2011)
  • Author(s): Jonsson, F., Mancardi, D. A., Kita, Y., Karasuyama, H., Iannascoli, B., Van Rooijen, N., Shimizu, T., Daeron, M, and Bruhns, P.
  • Journal Title: J. Clin. Invest Volume: 121 Pages: 1484-1496
  • Peer Reviewed
• [Journal Article] NK cell-depleting anti-asialo GM1 Ab exhibits a lethal off-target effect on basophils in vivo (2011)
  • Author(s): Nishikado, H., Mukai, K., Kawano, Y., Minegishi, Y., and Karasuyama, H.
  • Journal Title: J. Immunol Volume: 186 Pages: 5766-5771
  • Peer Reviewed
• [Journal Article] Newly appreciated roles for basophils in allergy and protective immunity (2011)
  • Author(s): Karasuyama, H., Obata, K., Wada, T., Tsujimura, Y., and Mukai, K.
  • Journal Title: Allergy Volume: 66 Pages: 1133-1141
  • Peer Reviewed
• [Journal Article] Critical role of the neutrophil-associated high-affinity receptor for IgE in the pathogenesis of experimental cereberal malaria (2011)
  • Author(s): Porcherie, A., Mathieu, C., Peronet, R., Schneider, E., Claver, J., Commere, P-H., Kiefer-Biasizzo, H., Karasuyama, H., Milon, G., Dy, M., Kinet, J-P., Louis, J., Blank, U., and Mecheri, S.
  • Journal Title: J. Exp. Med Volume: 208 Pages: 2225-2236
  • Peer Reviewed
• [Journal Article] Basophil-derived mouse mast cell protease 11 induces microvascular leakage and tissue edema in a mast cell-independent manner (2011)
  • Author(s): Yamagishi, H., Mochizuki, Y., Hamakubo, T., Obata, K., Ugajin, T., Sato, S., Kawano, Y., Minegishi, Y., and Karasuyama, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 415 Pages: 709-713
  • Peer Reviewed
• [Journal Article] 最近クローズアップされた好塩基球の重要性 (2011)
  • Author(s): 烏山一
  • Journal Title: 感染・炎症・免疫 Volume: 40(4) Pages: 12-21
• [Journal Article] アレルギーと生体防御における好塩基球の新たな役割 (2011)
  • Author(s): 烏山一
  • Journal Title: アレルギー・免疫 Volume: 18(4) Pages: 42-49
• [Journal Article] 慢性アレルギーと好塩基球 (2011)
  • Author(s): 小畑一茂、石川亮、烏山一
  • Journal Title: 慢性炎症-多様な疾患の基盤病態,実験医学増刊号 Volume: 29(10) Pages: 151-155
• [Journal Article] 好塩基球の研究の進歩 (2011)
  • Author(s): 江川真由美、烏山一
  • Journal Title: 血液フロンティア Volume: 21(6) Pages: 92-95
• [Journal Article] The transfer of maternal antigen-specific IgG regulates the development of allergic airway inflammation early in life in an FcRn-dependent manner (2010)
  • Author(s): Nakata, K., Kobayashi, K., Ishikawa, Y., Yamamoto, M., Funada, Y., Kotani, Y., Blumberg, R. S., Karasuyama, H., Yoshida, M., and Yoshihiro Nishimura.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 395 Pages: 238-243
  • Peer Reviewed
• [Journal Article] Role for basophils in systemic anaphylaxis (2010)
  • Author(s): Karasuyama, H., Tsujimura Y., Obata, K., and Mukai, K.
  • Journal Title: Chem. Immunol. Allergy Volume: 95 Pages: 85-97
  • Peer Reviewed
• [Journal Article] Selective ablation of basophils in mice reveals their nonredundant role in acquired immunity against ticks (2010)
  • Author(s): Wada, T., Ishiwata, K., Koseki, H., Ishikura, T., Ugajin, T., Ohnuma, N., Obata, K., Ishikawa, R., Yoshikawa, S., Mukai, K., Kawano, Y., Minegishi, Y., Yokozeki, H., Watanabe, N., and Karasuyama, H.
  • Journal Title: J. Clin. Invest Volume: 120 Pages: 2867-2875
  • Peer Reviewed
• [Journal Article] An atopic dermatitis-like skin disease with hyper-IgEemia develops in mice carrying a spontaneous recessive point mutation in the Traf3ip2(Act1/CIKS) gene (2010)
  • Author(s): Matsushima, Y., Kikkawa, Y., Takada, T, Matsuoka, K., Seki, Y., Yoshida, H., Minegishi, Y., Karasuyama, H., and Yonekawa, H.
  • Journal Title: J. Immunol Volume: 185 Pages: 2340-2349
  • Peer Reviewed
• [Journal Article] Nonredundant roles for basophils in immediate-and delayed-onset allergic reactions (2010)
  • Author(s): Karasuyama, H., Mukai, K., Obata, K., Tsujimura, Y., Kawano, Y., and Minegishi, Y.
  • Journal Title: The Open Allergy Journal Volume: 3 Pages: 73-80
  • Peer Reviewed
• [Journal Article] Basophils amplify type 2 immune responses, but do not serve a protective role, during chronic infection of mice with the filarial nematode (2010)
  • Author(s): Torrero, M. N., Hubner, M. P., Larson, D., Karasuyama, H., and Mitre, E.
  • Journal Title: Litomosoides sigmodontis. J. Immunol Volume: 185 Pages: 7426-7434
  • Peer Reviewed
• [Journal Article] IgG-mediated systemic anaphylaxis to protein antigen can be induced even under conditions of limited amounts of antibody and antigen (2010)
  • Author(s): Ishikawa, R., Tsujimura, Y., Obata, K., Kawano, Y., Minegishi, Y., and Karasuyama, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 402 Pages: 742-746
  • Peer Reviewed
• [Journal Article] 急性ならびに慢性アレルギー発症における好塩基球の新たな役割 (2010)
  • Author(s): 烏山一
  • Journal Title: 炎症と免疫 Volume: 18(1) Pages: 23-27
• [Journal Article] 好塩基球が主役を演じるIgE依存性慢性アレルギー炎症 (2010)
  • Author(s): 烏山一
  • Journal Title: IgE practice in asthma Pages: 1-5
• [Journal Article] 吸血ダニに対する抵抗性、好塩基球が鍵を握っていた (2010)
  • Author(s): 烏山一
  • Journal Title: メディカルバイオ Volume: 7(6) Pages: 52-55
• [Journal Article] Newly-discovered roles for basophils : a neglected minority gains new respect (2009)
  • Author(s): Karasuyama, H., Mukai, K., Tsujimura, Y. and Obata, K.
  • Journal Title: Nat. Rev. Immunol Volume: 9 Pages: 9-13
  • Peer Reviewed
• [Journal Article] Fc receptorγ-chain, a constitutive component of the interleukin 3 receptor is required for interleukin 3-induced interleukin 4 (2009)
  • Author(s): Hida, S., Yamasaki, S., Sakamoto, Y., Takamoto, M., Obata, K., Takai, T., Karasuyama, H., Sugane, K., Saito, T. and Taki, S.
  • Journal Title: Nat. Immunol Volume: 10 Pages: 214-222
  • Peer Reviewed
• [Journal Article] New insights into the roles for basophils in acute and chronic allergy (2009)
  • Author(s): Mukai, K., Obata, K., Tsujimura, Y. and Karasuyama, H.
  • Journal Title: Allergol. Int Volume: 58 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Peanuts can contribute to anaphylactic shock by activating complement (2009)
  • Author(s): Khodoun, M., Strait, R., Orekov, T., Hogan, S., Karasuyama, H., Herbert, D. R., Kohl, J. and Finkelman, F. D.
  • Journal Title: J. Allergy Clin. Immunol Volume: 58 Pages: 11-19
  • Peer Reviewed
• [Journal Article] Basophils preferentially express mouse mast cell protease 11 among the mast cell tryptase family in contrast to mast cells (2009)
  • Author(s): Ugajin, T., Kojima, T., Mukai, K., Obata K., Kawano, Y., Minegishi, Y., Eishi Y., Yokozeki, H., and Karasuyama, H.
  • Journal Title: J. Leukoc. Biol Volume: 86 Pages: 1417-1425
  • Peer Reviewed
• [Journal Article] 好塩基球とアレルギー (2009)
  • Author(s): 向井香織、小畑一茂、辻村祐佑、烏山一
  • Journal Title: 日本臨床 Volume: 67(11) Pages: 2095-2009
• [Journal Article] アレルギーにおける好塩基球の新たな役割 (2009)
  • Author(s): 烏山一
  • Journal Title: 実験医学増刊「アレルギー疾患の免疫機構」 Volume: 27(20) Pages: 68-74
• [Journal Article] アレルギーにおける好塩基球の新たな役割 (2009)
  • Author(s): 烏山一
  • Journal Title: Annual Review 2009呼吸器 Pages: 44-51
• [Journal Article] 免疫アレルギー研究の表舞台に躍り出た好塩基球 (2009)
  • Author(s): 烏山一
  • Journal Title: アレルギー・免疫 Volume: 16(3) Pages: 9-10
• [Journal Article] CD200R3を介した好塩基球とマスト細胞の活性化 (2009)
  • Author(s): 小島利之、小畑一茂、烏山一
  • Journal Title: アレルギー・免疫 Volume: 16(3) Pages: 44-51
• [Journal Article] 好塩基球によるIgG型アナフィラキシーの誘導 (2009)
  • Author(s): 辻村祐佑、小畑一茂、烏山一
  • Journal Title: アレルギー・免疫 Volume: 16(3) Pages: 62-71
• [Journal Article] 慢性アレルギー炎症ならびにアナフィラキシーにおける好塩基球のあらたな役割 (2009)
  • Author(s): 烏山一
  • Journal Title: 医学のあゆみ別冊「最新免疫研究Update」 Pages: 77-82
• [Journal Article] 好塩基球が主役を演じる慢性アレルギー炎症 (2009)
  • Author(s): 向井香織、烏山一
  • Journal Title: アレルギー・免疫 Volume: 16(3) Pages: 72-77
• [Journal Article] 好塩基球・マスト細胞の機能とCD200R3 (2009)
  • Author(s): 小島利之、小畑一茂、向井香織、烏山一
  • Journal Title: 臨床免疫アレルギー科 Volume: 52(1) Pages: 49-57
• [Journal Article] Emerging roles of basophils in protective immunity against parasites
  • Author(s): Karasuyama, H., Wada, T., Yoshikawa, S., and Obata, K.
  • Journal Title: Trends Immunol Volume: 32 Pages: 125-130
  • Peer Reviewed
• [Presentation] Emerging roles of basophils in protective and pathological immune responses (2011)
  • Author(s): Karasuyama, H.
  • Organizer: EMBRN-COST International Mast Cell and Basophil Meeting
  • Place of Presentation: Southampton, UK
  • Year and Date: 2011-11-25
• [Presentation] Newly appreciated roles for basophils in allergy and protective immunity. Japan Korea China Joint Symposium' Inflammatory cells in allergic diseases' (2011)
  • Author(s): Karasuyama, H.
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Year and Date: 2011-11-10
• [Presentation] Emerging roles of basophils in protective and pathological immune responses (2011)
  • Author(s): Karasuyama, H.
  • Organizer: JSICR MMCB 2011
  • Place of Presentation: Osaka
  • Year and Date: 2011-05-26
• [Presentation] Basophils as a non-redundant player in allergy and protective immunity (2010)
  • Author(s): Karasuyama, H.
  • Organizer: The 20^<th> Annual Johns Hopkins Asthma and Allergy Center Symposium
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2010-11-19
• [Presentation] Novel roles for basophils in allergy and protective immunity (2010)
  • Author(s): Karasuyama, H.
  • Organizer: Cold Spring Harbor-Asia Conference' Frontiers of Immunology in Health & Diseases'
  • Place of Presentation: Suzhou, China
  • Year and Date: 2010-11-09
• [Presentation] Newly discovered roles for basophils : a neglected minority gains new respect (2010)
  • Author(s): Karasuyama, H.
  • Organizer: The 40th Annual Meeting of the German Society for Immunology
  • Place of Presentation: Leipzig, Germany
  • Year and Date: 2010-09-23
• [Presentation] Novel roles for basophils in allergy and immunity (2010)
  • Author(s): Karasuyama, H.
  • Organizer: International Congress of Immunology 2010
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-26
• [Presentation] Novel roles for immune and allergic responses (2010)
  • Author(s): Karasuyama, H.
  • Organizer: The 97^<th> Annual Meeting of the American Association of Immunologists
  • Place of Presentation: Baltimore, USA.
  • Year and Date: 2010-05-08
• [Presentation] Novel roles for basophilsin allergy. International Symposium ; Eosinophils, other inflammatory cells and molecules in allergy (2009)
  • Author(s): Karasuyama, H.
  • Organizer: 第59回日本アレルギー学術秋季学術大会
  • Place of Presentation: 秋田
  • Year and Date: 2009-10-30
• [Presentation] Newly discovered roles for basophils-a neglected minority gains new respect (2009)
  • Author(s): Karasuyama, H.
  • Organizer: 6th Biennial International Symposium of the International Eosinophil Society
  • Place of Presentation: Bruges, Belgium
  • Year and Date: 2009-07-08
• [Book] Environmental and genetic factors in allergy and clinical immunology (2010)
  • Author(s): Karasuyama, H., Tsujimura Y., Obata, K., Mukai, K., and Minegishi Y.
  • Total Pages: 63-65
  • Publisher: Basophils and mast cells play distinct roles in acute and chronic allergic reactions
• [Book] 「粘膜における自然免疫:肥満細胞・好酸球・好塩基球」臨床粘膜免疫学 (2010)
  • Author(s): 斎藤博久、大保木啓介、中江進、烏山一
  • Total Pages: 79-88
  • Publisher: シナジー社
• [Remarks]
  • URL: http://www.tmd.ac.jp/med/mbch/top_Japanese.htm