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2010 Fiscal Year Annual Research Report

Apopタンパク質による老化誘導機構の解析

Research Project

Project/Area Number 21390222
Research InstitutionOsaka University

Principal Investigator

樂木 宏実  大阪大学, 医学系研究科, 教授 (20252679)

Co-Investigator(Kenkyū-buntansha) 安田 修  熊本大学, 医学部附属病院, 特任准教授 (00372615)
福尾 惠介  武庫川女子大学, 生活環境学部, 教授 (40156758)
大石 充  大阪大学, 医学系研究科, 講師 (50335345)
Keywordsミトコンドリア / Apop
Research Abstract

Apop遺伝子をヘテロに欠損したマウスの交配により、Apopノックアウト(ApopKO)マウスを得た。ApopKOマウス同士の交配を行った結果、産仔の外観、体重は野生型と同等であったものの出生数は野生型に比して少なく、発生におけるApop遺伝子の関与と重要性を示す結果であった。
培養血管内皮細胞を高グルコース条件下で培養した結果、ミトコンドリアの機能低下が見られ、Apop発現ならびに酸化ストレス産生の上昇は上昇していた。この細胞ではβガラクトシダーゼ発現が見られ、細胞の老化が促進されていることを示すものであった。また発現ベクターを用いてApop遺伝子を発現した細胞では酸化ストレスの産生が亢進し、逆にsiRNAを用いてApop発現を抑制した細胞では酸化ストレス産生が抑制されていた。これらの結果はApop遺伝子が内皮細胞の酸化ストレス産生と傷害とをもたらしていることを示すとともに、Apop遺伝子は糖尿病による血管老化を促進していることを示している。以上よりApopが動脈硬化治療の標的になりうることが明らかとなった。またApop遺伝子発現を抑制した細胞ではミトコンドリア代謝とATP産生の上昇が認められ、Apopのミトコンドリア機能への関与を示していた。
老化疾患の代表である心筋梗塞後に治療のための再還流を行ったマウスでApop遺伝子の発現定量を行った結果、虚血再還流後4-6時間をピークにApop遺伝子発現の変化が認められた。この結果はApop遺伝子発現が心筋梗塞治療後の再還流による細胞死と酸化ストレス産生に関わっていることを示している。

  • Research Products

    (43 results)

All 2011 2010 Other

All Journal Article (30 results) (of which Peer Reviewed: 30 results) Presentation (9 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Usefulness of the resistive index in renal Doppler ultrasonography as an indicator of vascular damage in patients with risks of atherosclerosis2011

    • Author(s)
      Kawai T
    • Journal Title

      Nephrol Dial Transplant

      Volume: 26(10) Pages: 3256-3262

    • Peer Reviewed
  • [Journal Article] β-Adrenergic receptor gene polymorphism is a genetic risk factor for cardiovascular disease : a cohort study with hypertensive patients2011

    • Author(s)
      Iwamoto Y
    • Journal Title

      Hypertens Res

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] Role of Insulin Signaling in the Interaction Between Alzheimer Disease and Diabetes Mellitus : A Missing Link to Therapeutic Potential2011

    • Author(s)
      Sato N
    • Journal Title

      Curr Aging Sci

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] Association of (pro)renin receptor gene polymorphisms with lacunar infarction and left ventricular hypertrophy in Japanese women2011

    • Author(s)
      Hirose T
    • Journal Title

      Hypertens Res

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] CD28 superagonist-induced regulatory T cell expansion ameliorates mesangioproliferative glomerulonephritis in rats2011

    • Author(s)
      Miyasato K
    • Journal Title

      Clin Exp Nephrol

      Volume: 15 Pages: 50-57

    • Peer Reviewed
  • [Journal Article] Effects of chronic kidney disease and post-angiographic acute kidney injury on long-term prognosis after coronary artery angiography2011

    • Author(s)
      Kimura T
    • Journal Title

      Nephrol Dial Transplant

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] Impact of concomitant diabetes and chronic kidney disease on preload-induced changes in left ventricular diastolic filling in hypertensive patients2011

    • Author(s)
      Iwashima Y
    • Journal Title

      J Hypertens

      Volume: 29 Pages: 144-153

    • Peer Reviewed
  • [Journal Article] Hepatocyte growth factor attenuates renal fibrosis through TGF-β1 suppression by apoptosis of myofibroblasts2011

    • Author(s)
      Iekushi K
    • Journal Title

      J Hypertens

      Volume: 28 Pages: 2454-2461

    • Peer Reviewed
  • [Journal Article] Urinary type IV collagen in nondiabetic kidney disease2011

    • Author(s)
      Furumatsu Y
    • Journal Title

      Nephron Clin Pract

      Volume: 117 Pages: c160-166

    • Peer Reviewed
  • [Journal Article] Promoter Polymorphism of RGS2 Gene Is Associated with Change of Blood Pressure in Subjects with Antihypertensive Treatment2010

    • Author(s)
      Sugimoto K
    • Journal Title

      Int J Hypertens

      Volume: 2010 Pages: 196307-196313

    • Peer Reviewed
  • [Journal Article] Fetuin-mineral complex reflects extraosseous calcification stress in CKD2010

    • Author(s)
      Hamano T
    • Journal Title

      J Am Soc Nephrol

      Volume: 21 Pages: 1998-2007

    • Peer Reviewed
  • [Journal Article] The counterregulating role of ACE2 and ACE2-mediated angiotensin 1-7 signaling against angiotensin II stimulation in vascular cells2010

    • Author(s)
      Hayashi N
    • Journal Title

      Hypertens Res

      Volume: 33 Pages: 1182-1185

    • Peer Reviewed
  • [Journal Article] Comparison of strict- and mild-blood pressure control in elderly hypertensive patients : a per-protocol analysis of JATOS2010

    • Author(s)
      Rakugi H
    • Journal Title

      Hypertens Res

      Volume: 33 Pages: 1124-1128

    • Peer Reviewed
  • [Journal Article] Identification of evidence suggestive of an association with peripheral arterial disease at the OSBPL10 locus by genome-wide investigation in the Japanese population2010

    • Author(s)
      Koriyama H
    • Journal Title

      J Atheroscler Thromb

      Volume: 17 Pages: 1054-1062

    • Peer Reviewed
  • [Journal Article] ANG II inhibits insulin-mediated production of PI 3,4,5-trisphosphates via a Ca2+-dependent but PKC-independent pathway in the cardiomyocytes2010

    • Author(s)
      Ikushima M
    • Journal Title

      Am J Physiol Heart Circ Physiol

      Volume: 299 Pages: H680-689

    • Peer Reviewed
  • [Journal Article] Estrogen inhibits vascular calcification via vascular RANKL system : common mechanism of osteoporosis and vascular calcification2010

    • Author(s)
      Osako MK
    • Journal Title

      Circ Res

      Volume: 107 Pages: 466-475

    • Peer Reviewed
  • [Journal Article] Impact of age and overt proteinuria on outcomes of stage 3 to 5 chronic kidney disease in a referred cohort2010

    • Author(s)
      Obi Y
    • Journal Title

      Clin J Am Soc Nephrol

      Volume: 5 Pages: 1558-1565

    • Peer Reviewed
  • [Journal Article] Valsartan in Elderly Isolated Systolic Hypertension Study Group. Target blood pressure for treatment of isolated systolic hypertension in the elderly : valsartan in elderly isolated systolic hypertension study2010

    • Author(s)
      Ogihara T
    • Journal Title

      Hypertension

      Volume: 56 Pages: 196-202

    • Peer Reviewed
  • [Journal Article] Nifedipine inhibits vascular smooth muscle cell dedifferentiation via downregulation of Akt signaling2010

    • Author(s)
      Kaimoto T
    • Journal Title

      Hypertension

      Volume: 56 Pages: 247-252

    • Peer Reviewed
  • [Journal Article] Reduction of brain beta-amyloid (Abeta) by fluvastatin, a hydroxymethylglutaryl-CoA reductase inhibitor, through increase in degradation of amyloid precursor protein C-terminal fragments (APP-CTFs) and Abeta clearance2010

    • Author(s)
      Shinohara M
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 22091-22102

    • Peer Reviewed
  • [Journal Article] Cigarette smoking and progression of IgA nephropathy2010

    • Author(s)
      Yamamoto R
    • Journal Title

      Am J Kidney Dis

      Volume: 56 Pages: 313-324

    • Peer Reviewed
  • [Journal Article] Involvement of microsomal triglyceride transfer protein in nonalcoholic steatohepatitis in novel spontaneous mouse model2010

    • Author(s)
      Shindo N
    • Journal Title

      J Hepatol

      Volume: 52 Pages: 903-912

    • Peer Reviewed
  • [Journal Article] Association of carotid atherosclerosis with genetic polymorphisms of the klotho gene in patients with hypertension2010

    • Author(s)
      Oguro R
    • Journal Title

      Geriatr Gerontol Int

      Volume: 10 Pages: 311-318

    • Peer Reviewed
  • [Journal Article] The role of sympathetic nervous activity in renal injury and end-stage renal disease2010

    • Author(s)
      Masuo K
    • Journal Title

      Hypertens Res

      Volume: 33 Pages: 521-528

    • Peer Reviewed
  • [Journal Article] Diabetes-accelerated memory dysfunction via cerebrovascular inflammation and Abeta deposition in an Alzheimer mouse model with diabetes2010

    • Author(s)
      Takeda S
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 107 Pages: 7036-7041

    • Peer Reviewed
  • [Journal Article] Variation in OSBPL10 is associated with dyslipidemia2010

    • Author(s)
      Koriyama H
    • Journal Title

      Hypertens Res

      Volume: 33 Pages: 511-514

    • Peer Reviewed
  • [Journal Article] Campylobacter fetus Peritonitis in a Patient with an Unused Embedded Subcutaneous Peritoneal Catheter2010

    • Author(s)
      Inoue K
    • Journal Title

      Perit Dial Int

      Volume: 30 Pages: 253-254

    • Peer Reviewed
  • [Journal Article] Loss of ACE2 accelerates time-dependent glomerular and tubulointerstitial damage in streptozotocin-induced diabetic mice2010

    • Author(s)
      Shiota A
    • Journal Title

      Hypertens Res

      Volume: 33 Pages: 298-307

    • Peer Reviewed
  • [Journal Article] Estrogen attenuates vascular remodeling in Lp(a) transgenic mice2010

    • Author(s)
      Nakagami F
    • Journal Title

      Atherosclerosis

      Volume: 211 Pages: 41-47

    • Peer Reviewed
  • [Journal Article] Specialist care and improved long-term survival of dialysis patients2010

    • Author(s)
      Furumatsu Y
    • Journal Title

      Nephrol Dial Transplant

      Volume: 25 Pages: 1930-1935

    • Peer Reviewed
  • [Presentation] 高血圧と血管老化2010

    • Author(s)
      樂木宏実
    • Organizer
      第18回日本血管生物医学会学術集会
    • Place of Presentation
      大阪(招待講演)
    • Year and Date
      2010-12-01
  • [Presentation] 老年医学の新しい展望2010

    • Author(s)
      樂木宏実
    • Organizer
      第21回老年医学会北陸地方会
    • Place of Presentation
      福井(招待講演)
    • Year and Date
      2010-11-13
  • [Presentation] VALISH studyから2010

    • Author(s)
      樂木宏実
    • Organizer
      第33回日本高血圧学会総会
    • Place of Presentation
      福岡(招待講演)
    • Year and Date
      2010-10-15
  • [Presentation] VALISHからのレッスン2010

    • Author(s)
      樂木宏実
    • Organizer
      第33回日本高血圧学会総会
    • Place of Presentation
      福岡(招待講演)
    • Year and Date
      2010-10-15
  • [Presentation] ガイドライン改訂の課題2010

    • Author(s)
      樂木宏実
    • Organizer
      第33回日本高血圧学会総会
    • Place of Presentation
      福岡(招待講演)
    • Year and Date
      2010-10-15
  • [Presentation] 本態性高血圧-成因・診断・治療-2010

    • Author(s)
      樂木宏実
    • Organizer
      老年医学会 北陸地方会
    • Place of Presentation
      福井(招待講演)
    • Year and Date
      2010-10-13
  • [Presentation] Optimal Target Blood Pressure for Elderly Hypertensive Patients2010

    • Author(s)
      樂木宏実
    • Organizer
      The 23^<rd> scientific meeting of the International Society of Hypertension (ISH2010)
    • Place of Presentation
      バンクーバー(カナダ)(招待講演)
    • Year and Date
      2010-09-27
  • [Presentation] 高齢者高血圧の治療指針2010

    • Author(s)
      樂木宏実
    • Organizer
      第52回日本老年医学会学術集会
    • Place of Presentation
      神戸(招待講演)
    • Year and Date
      2010-06-24
  • [Presentation] 糖尿病患者の高血圧治療と糖尿病発症予防の高血圧治療2010

    • Author(s)
      樂木宏実
    • Organizer
      第53回日本糖尿病学会年次学術集会
    • Place of Presentation
      岡山(招待講演)
    • Year and Date
      2010-05-28
  • [Book] 内分泌性高血圧診療マニュアル2010

    • Author(s)
      樂木宏実
    • Total Pages
      44-49
    • Publisher
      アンジオテンシン変換酵素(ACE)
  • [Book] 内分泌性高血圧診療マニュアル2010

    • Author(s)
      樂木宏実
    • Total Pages
      5-6
    • Publisher
      高血圧治療ガイドラインと内分泌性高血圧
  • [Book] 内分泌性高血圧診療マニュアル2010

    • Author(s)
      樂木宏実
    • Total Pages
      78-79
    • Publisher
      低カリウムを伴う高血圧の鑑別診断
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/geriat/www/

URL: 

Published: 2012-07-19  

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