2011 Fiscal Year Final Research Report
Basic research for the production of human hepatocytes and the transplantation of hepatic tissues
| Project/Area Number |
21390365
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANIMIZU Naoki 札幌医科大学, 医学部, 講師 (00333386)
OOE Hidekazu 札幌医科大学, 医学部, 助教 (90452979)
ICHINOHE Norihisa 札幌医科大学, 医学部, 研究員 (80452978)
MIZUGUCHI Toru 札幌医科大学, 医学部, 准教授 (30347174)
HIRATA Koichi 札幌医科大学, 医学部, 教授 (50136959)
KIKKAWA Yamato 東京薬科大学, 薬学部, 准教授 (20274227)
TANISHITA Kazuo 慶應義塾大学, 理工学部, 教授 (10101776)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 肝再生 / 肝幹・前駆細胞 / 細胞移植 / 増殖 / 分化 / 肝組織形成 / 肝発生 / 人工肝臓 |
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| Research Abstract |
We aimed to develop the methods of producing a large number of small hepatocytes(SHs), hepatic progenitor cells, and to establish the methods of hepatic organoid formation in vitro. In addition, we examined how cells and tissues could be transplanted into liver. Unlike mature hepatocytes the growth of rat SHs was regulated by Follistatin/Activin system. Although oval cells, hepatic stem cells, differentiated into hepatocytes via SHs, they could not completely mature. The transplanted hepatic stem/progenitor cells could not survive for a long term in the recipient liver and disappeared by cellular senescence. Thus, the role of hepatic stem/progenitor cells in severe liver failure may be to compensate for the lost hepatic functions transiently. To use the model of hepatic sinusoids constituting rat SHs, stellate cells, and endothelial cells, we clarified that quiescent stellate cells might regulate the capillary formation of endothelial cells and maturation of SHs.
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