2011 Fiscal Year Final Research Report
Proteomic analysis of global changes induced by deep hypothermia in both protein synthesis and phosphorylation.
| Project/Area Number |
21390389
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Shimane University |
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Principal Investigator |
ODA Teiji 島根大学, 医学部, 教授 (50448198)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Kenichi 島根大学, 総合科学研究支援センター, 教授 (30202328)
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| Co-Investigator(Renkei-kenkyūsha) |
TOYOTA Kousaku 島根大学, 医学部, 助教 (30322225)
NIKAI Tetsurou 島根大学, 医学部, 助教 (20314643)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 心臓大血管外科学 / 低体温療法 / プロテオミクス解析 / 冬眠 |
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| Research Abstract |
Hypothermia is utilized with increasing frequency to protect organs such as the brain against ischemic injury. Though a principal mechanism of hypothermic protection would be suppression of metabolism, its pleiotropic effects are as yet incompletely understood. To analyze hypothermia-induced proteomic changes, we employed fluorescence-based two-dimensional(2-D) difference gel electrophoresis(DIGE) and matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF/TOF) tandem mass spectrometry. Deep hypothermia produced significant down-regulation of the proteins that regulates ATP synthesis or muscle contraction in rat heart muscle. Hypothermia also produced significant down-regulation of the following cellular components and biological processes or pathways in rat livers : endoplasmic reticulum(ER), mitochondria, ATP binding, amino acid metabolism, urea cycle, response to oxidative stress, anti-apoptosis, negative regulation of apoptosis. These changes were similar to those in hibernating animals except for both ER and apoptosis-related pathways, which are conversely up-regulated in hibernating mammals. This discrepancy needs to be investigated further in future research.
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Research Products
(5 results)
