Transplantation of iPS cell derived neural stem cells for spinal cord injury

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390426
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: NAKAMURA Masaya 慶應義塾大学, 医学部, 講師 (30217898)
• Co-Investigator(Kenkyū-buntansha): TOYAMA Yoshiaki 慶應義塾大学, 医学部, 教授 (40129549)
• Co-Investigator(Renkei-kenkyūsha): ISHII Ken 慶應義塾大学, 医学部, 講師 (00276289)
• Project Period (FY): 2009 – 2011
• Keywords: 脊髄損傷 / 人工多能性幹細胞 / 移植 / 再生医療

Research Abstract

In the present study, we demonstrated that transplantation of mouse induced pluripotent stem cells(iPSC) derived neural stem/progenitor cells(NS/PCs) promoted functional recovery after spinal cord injury(SCI) in mice. Furthermore, transplantation of human iPSC-NS/PC also promoted functional recovery after SCI in NOD/scid mice as well as common marmosets without any tumor formation as long as pre-evaluated''safe" human iPSC-NS/PCs were used as a cell source. Pre-evaluated'safe' human iPSC-NS/PCs s may be a promising cell source for transplantation therapy for patients with SCI.

Research Products

• [Journal Article] Cell therapy for spinal cord injury by neural stem/progenitor cells derived from iPS/ES cells (2011)
  • Author(s): Tsuji O, Miura K, Fujiyoshi K, Momoshima S, Nakamura M, Okano H.
  • Journal Title: Neurotherapeutics Volume: 8 Pages: 668-676
  • Peer Reviewed
• [Journal Article] Toward using iPS cells to treat spinal cord injury : their safety and therapeutic efficacy (2011)
  • Author(s): Miura K, Tsuji O, Nakamura M, Okano H
  • Journal Title: Inflammation and Regeneration Volume: 31 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Grafted human-induced pluripotent stem cell. derived neurospheres promote motor functional recovery after spinal cord injury in mice (2011)
  • Author(s): Nori S, Okada Y, Yasudaa A, Tsuji O, Takahashi Y, Kobayashia Y, Fujiyoshi K, Koike M, Uchiyama Y, Ikeda E, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Journal Title: Pro Nat Acad Sci USA Volume: 108 Pages: 16825-16830
  • Peer Reviewed
• [Journal Article] Significance of re-myelination by neural stem/progenitor cells transplanted into the injured spinal cord (2011)
  • Author(s): Yasuda A, Tsuji O, Shibata S, Nori S, Takano M, Kobayashi Y, Takahashi Y, Fujiyoshi K, Miyauchi Hara C, Miyawaki A, Okano JH, Toyama Y, Nakamura M, Okano H
  • Journal Title: Stem Cells Volume: 29 Pages: 1983-1994
  • Peer Reviewed
• [Journal Article] Comparative study of methods for administering neural stem/progenitor cells to treat spinal cord injury in mice (2011)
  • Author(s): TakahashiY, Tsuji O, KumagaiG, Miyauchi Hara C, Okano JH, Miyawaki A, Toyama Y, Okano H, NakamuraM
  • Journal Title: Cell Transplant Volume: 20 Pages: 727-739
  • Peer Reviewed
• [Journal Article] Mimicking the neurotrophic factor profile of embryonic spinal cord controls the differentiation potential of spinal progenitors into neuronal cellsg (2011)
  • Author(s): Nakamura M, Tsuji O, Bregman BS, Toyama Y, Okano H
  • Journal Title: PLos ONE Volume: e20717 Pages: 6
  • DOI: doi:10.1371/journal.pone.0020717
  • Peer Reviewed
• [Journal Article] Transplantation of Galectin-1-Expressing Human Neural Stem Cells into the Injured Spinal Cord of Adult Common Marmosets (2010)
  • Author(s): Yamane J, Nakamura M, Iwanami A, Sakaguchi M, Katoh H, Yamada M, Momoshima S, Miyao S, Ishii K, Tamaoki N, Nomura T, Okano HJ, Kanemura Y, Toyama Y, Okano H
  • Journal Title: J Neurosci Res Volume: 88 Pages: 1394-1405
  • Peer Reviewed
• [Journal Article] Therapeutic potential of appropriately evaluated 'safe' induced pluripotent stem cells for spinal cord injury (2010)
  • Author(s): Tsuji O, Miura K, Okada Y, Fujiyoshi K, Mukaino M, Nagoshi N, Kitamura K, KumagaiG, NishinoM, Tomisato S, Higashi H, NagaiT, Katoh H, Kohda K, MatsuzakiY, YuzakiM, IkedaE, ToyamaY, NakamuraM, Yamanaka S, Okano H.
  • Journal Title: Pro Nat Acad Sci USA Volume: 107 Pages: 12704-12709
  • Peer Reviewed
• [Journal Article] Regenerative medicine for spinal cord injury : Current status and open issues (2009)
  • Author(s): Nakamura M, Nagoshi N, Fujiyoshi K, Kaneko S, Toyama Y, Okano H.
  • Journal Title: Inflammation and Regeneration Volume: 29 Pages: 198-203
  • Peer Reviewed
• [Presentation] Therapeutic potential of appropriately evaluated 'safe' induced pluripotent stem cells for spinal cord injury (2011)
  • Author(s): Tsuji O, Fujiyoshi K, Miura K, Matsumoto M, Chiba K, Toyama Y, Yamanaka S, Okano H, Nakamura M
  • Organizer: Cervical Spine Research Society 38^<th> Annual Meeting
  • Place of Presentation: Arizona, USA
  • Year and Date: 20111207-10
• [Presentation] Transplantation of human iPSC-derived neurospheres for the treatment of spinal cord injury in NOD-scid mice (2011)
  • Author(s): Nori S, Okada Y, Yasuda A, Tsuji O, Takahashi Y, Kobayashi Y, Fujiyoshi K, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Organizer: Cervical Spine Research Society 38^<th> Annual Meeting
  • Place of Presentation: Arizona, USA
  • Year and Date: 20111207-10
• [Presentation] Pre-evaluated safe human iPS clone derived neural stem cells promoted functional recovery after spinal cord injury without tumorigenicity in adult common marmosets (2011)
  • Author(s): Kobayashi Y, Okada Y, Iwai H, Nishimura S, Nori S, Konomi T, Fujiyoshi K, Tsuji O, Toyama Y, Yamanaka S, Okano H, Nakamura M
  • Organizer: Cervical Spine Research Society 38^<th> Annual Meeting, oral presentation
  • Place of Presentation: Arizona, USA
  • Year and Date: 20111207-10
• [Presentation] Pre-evaluated safe human iPSC-derived neurospheres promote functional recovery after spinal cord injury without tumorigenecity in adult common marmosets (2011)
  • Author(s): Kobayashi Y, Okada Y, Iwai H, Nishimura S, Nori S, Konomi T, Fujiyoshi K, Tsuji O, Toyama Y, Yamanaka S, Nakamura M, Okano H.
  • Organizer: 41^<st> Annual meeting Neuroscience 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111112-16
• [Presentation] Transplantation of human iPSC-derived neurospheres promote motor functional recovery after spinal cord injury (2011)
  • Author(s): Nori S, Okada Y, Yasuda A, Tsuji O, Takahashi Y, Kobayashi Y, Fujiyoshi K, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Organizer: NOD-scid mice41^<st> Annual meeting Neuroscience 2011
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20111112-16
• [Presentation] Pre-evaluated safe human iPS clone derived neural stem cells promoted functional recovery after spinal cord injury without tumorigenecity in adult common marmosets (2011)
  • Author(s): Kobayashi Y, Okada Y, Iwai H, Nishimura S, Nori S, Konomi T, Fujiyoshi K, Tsuji O, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Organizer: ISSCR 2011
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20110615-18
• [Presentation] Transplantation of human iPS cell-derived neurospheres for the treatment of spinal cord injury in nod-scid mice (2011)
  • Author(s): Nori S, Okada Y, Yasuda A, Tsuji O, Takahashi Y, Kobayashi Y, Fujiyoshi K, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Organizer: ISSCR 2011
  • Place of Presentation: Toronto, Canada
  • Year and Date: 20110615-18
• [Presentation] Transplantation of human iPS cell-derived neurospheres for the treatment of spinal cord injury in NOD-scid mice (2010)
  • Author(s): Nori S, Okada Y, Tsuji O, Takahashi Y, Fujiyoshi K, Yasuda A, Kobayashi Y, Toyama Y, Yamanaka S, Nakamura M, Okano H.
  • Organizer: 40^<th> Annual meeting Neuroscience 2010
  • Place of Presentation: San Diego
  • Year and Date: 20101113-17
• [Presentation] Therapeutic potential of appropriately evaluated 'safe' induced pluripotent stem cells for spinal cord injury (2010)
  • Author(s): Tsuji O, Miura K, Okada Y, Fujiyoshi K, Matsumoto M, Chiba K, Yamanaka S, Toyama Y, Nakamura M, Okano H
  • Organizer: 40^<th> Annual meeting Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 20101113-17
• [Presentation] Transplantation of human iPS cell-derived neurospheres for the treatment of spinal cord injury in NOD-scid mice (2010)
  • Author(s): Nori S, Okada Y, Tsuji O, Takahashi Y, Fujiyoshi K, Yasuda A, Kobayashi Y, Toyama Y, Yamanaka S, Nakamura M, Okano H.
  • Organizer: Neuro2010
  • Place of Presentation: Kobe
  • Year and Date: 20100902-04
• [Presentation] Transplantation of human iPS cell-derived neurospheres for the treatment of spinal cord injury in NOD-scid mice. GCOE幹細胞医学のための教育拠点成果報告シンポジウム (2010)
  • Author(s): Nori S, Okada Y, Tsuji O, Takahashi Y, Fujiyoshi K, Yasuda A, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Organizer: Global COE Program Symposium 2010 Education and Research Center for Stem Cell Medicine
  • Place of Presentation: 日慶應義塾大学信濃町キャンパス
  • Year and Date: 20100225-26
• [Presentation] Therapeutic effect of pre-evaluated 'safe' iPS derived neurospheres for spinal cord injury (2009)
  • Author(s): Tsuji O, Miura K, Fujiyoshi K, Chiba K, Yamanaka S, Okano H, MD, Toyama Y, Nakamura M
  • Organizer: Cervical Spine Research Society 37^<th> Annual Meeting, oral presentation
  • Place of Presentation: East Lancaster, USA
  • Year and Date: 20091203-05
• [Presentation] Transplantation of human iPS cell-derived neurospheres into injured spinal cord of NOD-scid mice (2009)
  • Author(s): Nori S, Okada Y, Tsuji O, Takahashi Y, Fujiyoshi K, Yasuda A, Shimada H, Toyama Y, Yamanaka S, Nakamura M, Okano H
  • Organizer: The 39th annual meeting of the Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 20091017-21