Clinical application of human ovarian tissue cryopreservation

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390455
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University
• Principal Investigator: KUJI Naoaki 慶應義塾大学, 医学部, 講師 (80169987)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Hiroshi 帯広畜産大学, 原虫病研究センター, 教授 (60333473) ; KASHIWAZAKI Naomi 麻布大学, 獣医学部, 教授 (90298232)
• Co-Investigator(Renkei-kenkyūsha): HAMATANI Toshio 慶應義塾大学, 医学部, 講師 (60265882) ; MOCHIMARU Yoshiyuki 慶應義塾大学, 医学部, 助教 (80445238) ; TAKANO Mitsuko 慶應義塾大学, 医学部, 助教 (20445240)
• Project Period (FY): 2009 – 2011
• Keywords: 移植・再生医療 / 医療・福祉 / 再生医学

Research Abstract

The efficacy of magnetic field supplementation was confirmed, since normal litter was born only from magnetic field supplemented cryopreserved ovarian tissue, and those litter were appeared to be fertile. Although ovaries from juvenile porcine were cryopreserved, there is no ovary with function after freeze-thaw, neither from cryopreservation with magnetic field supplementation or that without magnetic field supplementation. Freezing temperature of both distilled water and freezing medium was not moved by magnetic field supplementation.

Research Products

• [Journal Article] Effect of bovine cumulus-oocyte complexes-conditioned medium on in-vitro maturation of canine oocytes (2011)
  • Author(s): Abdel-Ghani, M., Abe, Y., Asano, T., Hamano, S. and Suzuki, H.
  • Journal Title: Reprod. Med. Biol Volume: 10 Pages: 43-49
  • Peer Reviewed
• [Journal Article] Cryopreservation of canine embryos (2011)
  • Author(s): Abe, Y., Suwa, Y., Asano, T., Ueta, Y. Y., Kobayashi, N., Ohshima, N., Shirasuna, S., Abdel-Ghani, M. A., Oi, M., Kobayashi, Y., Miyoshi, M., Miyahara, K. and Suzuki, H.
  • Journal Title: Biol. Reprod Volume: 84 Pages: 363-368
  • Peer Reviewed
• [Journal Article] Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD-SCID mice (2011)
  • Author(s): Kawase, Y. and Suzuki, H
  • Journal Title: Reprod. Med. Biol Volume: 10 Pages: 259-266
  • Peer Reviewed
• [Journal Article] Effect of intraperitoneal administration of desialylated erythropoietin on the follicul ar survival in cryopreserved canine ovaries after xenotransplantation (2011)
  • Author(s): Hirayama, Y., Inoue, K. and Suzuki, H.
  • Journal Title: J. Mamm. Ova Res Volume: 28 Pages: 143-147
  • Peer Reviewed
• [Journal Article] Expr ession of erythropoietic cytokines in alpha-tocopherol tran sfer protein knockout mice with murine malaria infection (2011)
  • Author(s): Herbas, M. S., Ueta, Y. Y., Ishibashi, K. and Suzuki, H.
  • Journal Title: Parasitol. Res Volume: 109 Pages: 1243-1250
  • Peer Reviewed
• [Journal Article] Sero epidemiological survey of Brucella canis infection in dogs in Japan using the tube agglutination test (2011)
  • Author(s): Tachibana, M., Kobayashi, N., Inokuma, H., Suzuki, H. and Watarai, M.
  • Journal Title: J. Jpn. Vet. Med. Assoc Volume: 64 Pages: 559-561
  • Peer Reviewed
• [Journal Article] Beneficial effect of hyperbaric oxygen therapy on the follicular survival of mouse ovary after transplantation (2011)
  • Author(s): Hayashi, S., Asano, T., Kakizaki, R. and Suzuki, H.
  • Journal Title: J. Reprod. Dev Volume: 58(In press)
  • Peer Reviewed
• [Journal Article] Scavenger receptor mediates systemic RNA interference in ticks (2011)
  • Author(s): Aung, K. M., Boldbaatar, D., Umemiya-Shira fuji, R., Liao, M., Xuan, X., Suzuki, H., Galay, R. L., Tanaka, T. and Fujisaki, K.
  • Journal Title: PLoS ONE Volume: 6(12) Pages: e28407
  • Peer Reviewed
• [Journal Article] In vitro maturation of canine oocytes co-cultured with bovine and canine granulosa cell monolayers (2011)
  • Author(s): Abdel-Ghani, M., Shimizu, T., Asano, T. and Suzuki, H
  • Journal Title: Theriogenology Volume: 77 Pages: 347-355
  • Peer Reviewed
• [Journal Article] 【不妊診療のすべて】ART(生殖補助医療)精子・卵子・卵巣の凍結保存とその安全性 (2011)
  • Author(s): 久慈直昭, 井上治, 福永朝子, 菅原かな, 小川誠司, 奥村典子, 山田満稔, 浜谷敏生, 吉村泰典
  • Journal Title: 産婦人科治療 Volume: 102巻 Pages: 495-500
• [Journal Article] マウス単一卵子のDNAマイクロアレイによる遺伝子発現解析とその応用 (2010)
  • Author(s): 持丸佳之、久慈直昭、山田満稔、奥村典子、高野光子、浜谷敏生, 他5名
  • Journal Title: 日本産科婦人科学会雑誌 Volume: 62(2) Pages: 344
• [Journal Article] 交流磁場発生装置を用いたブタ卵巣の超低温保存 (2009)
  • Author(s): 持丸佳之、久慈直昭、高野光子、山田満稔、浜谷敏生, 他3名
  • Journal Title: 日本産科婦人科雑誌 Volume: 61(2) Pages: 635
• [Presentation] Puppies from frozen embryos (2011)
  • Author(s): Suzuki, H
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: いわて県民情報交流センター・アイーナ,盛岡(岩手県)
  • Year and Date: 20110913-17
• [Presentation] Murine Plasmodium virulence is affected due to oxidative stress status of the host (2011)
  • Author(s): Herbas, S. M., Maggloire, N. and Suzuki, H.
  • Organizer: 17^<th> Japanese-German cooperative symposium on protozoan diseases
  • Place of Presentation: Nara Women's University, Nara
  • Year and Date: 20110912-14
• [Presentation] Follicular loss of cryopreserved canine ovary after xenotransplantation and its solution (2011)
  • Author(s): Suzuki, H
  • Organizer: The 12th International Congress on Reproductive Biomedicine(招聘講演)
  • Place of Presentation: Royan Institute, Tehran, The Islamic Republic of Iran
  • Year and Date: 20110907-09
• [Presentation] Cryopreservation of canine embryos (2011)
  • Author(s): Suzuki, H
  • Organizer: The 12th International Congress on Reproductive Biomedicine(招聘講演)
  • Place of Presentation: Royan Institute, Tehran, The Islamic Republic of Iran
  • Year and Date: 20110907-09
• [Presentation] Effect of hyperbaric oxygen therapy on the follicular loss of ovarian tissues after transplantation (2011)
  • Author(s): Suzuki, H., Hayashi, S. and Asano, T.
  • Organizer: 44th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Portland, Oregon, USA
  • Year and Date: 20110731-0804
• [Presentation] Processing of semen from an HIV-1-positive male and its use in the IVF-ICSI procedure-clinical efficacy (2011)
  • Author(s): Osamu INOUE, Naoaki KUJI, Tomoko FUKUNAGA, Seiji OGAWA, Kana SUGAWARA, Mitsutoshi YAMADA, Toshio HAMATANI, Hideji HANABUSA, Yasunori YOSHIMURA and Shingo KATO
  • Organizer: 27th Annual Meeting of the European Society of Human Reproductoin and Embryology
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 20110703-06
• [Presentation] マウスの卵巣移植における高圧酸素療法の卵胞数減少抑制効果について (2011)
  • Author(s): Hayashi, S., Asano, T. and Suzuki, H.
  • Organizer: 第52回日本哺乳動物卵子学会
  • Place of Presentation: 国際医療福祉大学本校
  • Year and Date: 20110521-22
• [Presentation] X、Y精子選別によるイヌの雌雄産み分け技術の開発-FISH法によるイヌ精子の性判別法の確立- (2011)
  • Author(s): Oi, M., Yamada, K., Hayakawa, H. and Suzuki, H.
  • Organizer: 第26回動物生殖工学研究会
  • Place of Presentation: 北里本館,東京
  • Year and Date: 2011-12-04
• [Presentation] 盲導犬適性と性格関連遺伝子多型との関連性 (2011)
  • Author(s): Ohshima, N., Kobayashi, N., Shirasuna, S., Suwa, Y. and Suzuki, H.
  • Organizer: 北海道実験動物研究会・日本実験動物技術者協会北海道支部合同学術集会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-07-09
• [Presentation] マウスの卵巣移植における高圧酸素療法の効果 (2011)
  • Author(s): Hayashi, S., Asano, T. and Suzuki, H.
  • Organizer: 北海道実験動物研究会・日本実験動物技術者協会北海道支部合同学術集会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-07-09
• [Presentation] Globalgene expression analysis in single oocytes from young and aged mice (2009)
  • Author(s): Naoaki Kuji, et al
  • Organizer: European Society of Human Reproduction and Embryology
  • Place of Presentation: Amstedam, Holland
  • Year and Date: 2009-07-01