Genetherapy for Head and Neck Cancer using carrier cell-based delivery of replication-selective adenoviral vector

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390462
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kobe University
• Principal Investigator: NIBU Ken-ichi 神戸大学, 医学研究科, 教授 (20251283)
• Co-Investigator(Kenkyū-buntansha): OTSUKI Naoki 神戸大学, 医学研究科, 准教授 (40343264) ; SHIRAKAWA Toshiro 神戸大学, 医学研究科, 准教授 (70335446)
• Project Period (FY): 2009 – 2011
• Keywords: 頭頸部癌 / 遺伝子治療 / アデノウイルス / ミドカイン / siRNA / Gene therapy

Research Abstract

While multimodal treatments have been performed for the treatment of head and neck cancers, the prognoses of the patients with advanced head and neck cancer are still poor. The estimated survival rates of those patients are around 30%. New strategies for the treatment for head and neck cancer are desirable. To address this issue, we developed the midkine promoter-driven replication-selective adenoviral vector containing siRNA for EGFR or VEGF. With this adenoviral vector, synergistic effect of oncolytic adenovirus and inhibition of oncogene via RNA interference is effect.

Research Products

• [Journal Article] E10A, an adenovirus-carrying endostatin gene, dramatically increased the tumor drug concentration of metronomic chemotherapy with low-dose cisplatin in a xenograft mouse model for head and neck squamous-cell carcinoma (2012)
  • Author(s): Adhim Z, Lin X, Huang W, Morishita N, Nakamura T, Yasui H, Otsuki N, Shigemura K, Fujisawa M, Nibu K, Shirakawa T
  • Journal Title: Cancer Gene Ther Volume: 19 Pages: 144-152
• [Journal Article] Multi-institutional analysis of solitary extramedullary plasmacytoma of the head and neck treated with curative radiotherapy (2012)
  • Author(s): Sasaki R, Yasuda K, Abe E, Uchida N, Kawashima M, Uno T, Fujiwara M, Shioyama Y, Kagam i Y, Shibamoto Y, Nakata K, Takada Y, Kawabe T, Uehara K, Nibu K, Yamada S
  • Journal Title: Int J Radiat Oncol Biol Phys Volume: 82 Pages: 626-34
• [Journal Article] Blowing ratio as an evaluation tool for velopharyngeal function after oral and oropharyngeal cancer resection (2012)
  • Author(s): Katoh C, Saitoh M, Tsuneyuki M, Tanimoto H, Hashikawa K, Tahara S, Otsuki N, Nibu K
  • Journal Title: Head Neck Volume: 32 Pages: 1012-8
• [Journal Article] In vitro and in vivo inhibitory effect of three Cox-2 inhibitors and epithelial-to-mesenchymal transition in human bladder cancer cell lines (2011)
  • Author(s): Adhim Z, Matsuoka T, Bito T, Shigemura K, Lee KM, Kawabata M, Fujisawa M, Nibu K, Shirakawa T
  • Journal Title: B r J Cancer Volume: 102 Pages: 393-402
• [Journal Article] Weekly paclitaxel in patients with recurrent or metastatic head a nd neck cancer (2011)
  • Author(s): T ahara M, Minami H, Hasegawa Y, Tomita K, Watanabe A, Nibu K, Fujii M, Onozawa Y, Kurono Y, Sagae D, Seriu T, Tsukuda M
  • Journal Title: Cancer Chemother Pharmacol Volume: 68 Pages: 769-76
• [Journal Article] Quality of life after neck dissection : a multicenter longitudinal study by the Japanese Clinical Study Group on Standardization of Treatment for Lymph Node Metastasis of Head and Neck Cancer (2010)
  • Author(s): Nibu K, Ebihara Y, Ebihara M, Kawabata K, Onitsuka T, Fujii T, Saikawa M
  • Journal Title: Int J Clin Oncol Volume: 15 Pages: 33-8
• [Journal Article] Depressed expression of Klotho and FGF receptor1in hyperplastic parathyroid glands from uremic patients (2010)
  • Author(s): Komaba H, Goto S, Fujii H, Hamada Y, Kobayashi A, Shibuya K, Tominaga Y, Otsuki N, Nibu K, Nakagawa K, Tsugawa N, Okano T, Kitazawa R, Fukagawa M, Kita T
  • Journal Title: Kidney Int Volume: 77 Pages: 232-8
• [Journal Article] Nuclear factor-kappaB expression as a novel marker of radioresistance in early-stage laryngeal cancer (2010)
  • Author(s): Yoshida K, Sasaki R, Nishimura H, Okamoto Y, Suzuki Y, Kawabe T, Saito M, Otsuki N, Hayashi Y, Soejima T, Nibu K, Sugimura K
  • Journal Title: Head Neck Volume: 32 Pages: 646-55
• [Journal Article] Outpatient administration of radioactive iodine after total thyroidectomy for pediatric thyroid cancer : a report of three cases (2009)
  • Author(s): T akano T, Yane K, Oue T, Otsuki N, Nibu K, Hidaka Y
  • Journal Title: Int J Pediatr Otorhinolaryngol Volume: 73 Pages: 1810-3
• [Journal Article] Cyclooxygenase2promoter-based replication-selective adenoviral vector for hypopharyngeal cancer (2009)
  • Author(s): Nakagawa T, Tanaka H, Shirakawa T, Gotoh A, Hayashi Y, Hamada K, Tsukuda M, Nibu K
  • Journal Title: Arch Otolaryngol Head Neck Surg Volume: 135 Pages: 282-6
• [Presentation] Serum and tissue metabolomics of head and neck cancer (2011)
  • Author(s): Yonezawa K, Otsuki N, Nibu K
  • Organizer: 11^<th> Japan-Taiwan Conference on Otolaryngology-Head and Neck Surgery
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2011-12-08
• [Presentation] Morishita N, Kawabata M, Nibu K. Gene silencing with siRNA targeting E6/E7 as a therapeutic intervention against Head and Neck Cancer containing HPV16 cell lines (2011)
  • Author(s): A dhim Z, Otsuki N, Shirakawa T
  • Organizer: San Francisco2011Annual Meeting of American Academy of Otolaryngology-Head and Neck Surgery
  • Place of Presentation: SanFrancisco
  • Year and Date: 2011-11-11
• [Presentation] E10A, an adenovirus carrying endostatin gene, enhanced anti-tumor effect of metronomic chemotherapy with low dose cisplatin in a xenograft mouse model for head and neck squamous cell carcinoma (2011)
  • Author(s): Adhim Z, Lin X, H uang W, Nakamura T, Yasui H, Otsuki N, Nibu K^1, Fujisawa M, Shirakawa T
  • Organizer: 14^<th> Annual Meeting of American Society of Gene Therapy and Cellular Therapy
  • Place of Presentation: Seattle
  • Year and Date: 2011-05-18
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/jibi/1.ht