2012 Fiscal Year Final Research Report

Molecular mechanism on anti-angiogenic effect of siRNA in choroidal neovascularization

Research Project

Project/Area Number	21390469
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Ophthalmology
Research Institution	Nagoya City University
Principal Investigator	OGURA Yuichiro 名古屋市立大学, 大学院・医学研究科, 教授 (70191963)
Co-Investigator(Kenkyū-buntansha)	YASUKAWA Tsutomu 名古屋市立大学, 大学院・医学研究科, 准教授 (00324632) NOZAKI Miho 名古屋市立大学, 大学院・医学研究科, 講師 (00295601) MATSUBARA Akihisa 名古屋市立大学, 大学院・医学研究科, 助教 (80336685)
Project Period (FY)	2009 – 2012
Keywords	加齢黄斑変性 / 脈絡膜新生血管 / アテロコラーゲン / VEGF / RNA 干渉(siRNA)
Research Abstract	Previous studies have shown that siRNAs could suppress angiogenesis via stimulation of toll-like receptor (TLR) -3. The purpose of this study was to determine the efficacy of atelocollagen to deliver siRNA without TLR3 stimulation in the laser-induced choroidal neovascularization (CNV) model. The mean CNV volumes were significantly smaller in the naked siRNA-Luc, naked siRNA-Vegfa, or siRNA-Vegfa /atelocollagen complex compared with PBS, atelocollagen, or siRNA-Luc/atelocollagen complex-injected mice (p < 0.05). These findings demonstrate that atelocollagen may deliver siRNA without non-specific TLR3 stimulation in the murine laser-CNV model.

(3 results)