Translational research of hard tissue regeneration using physiologically active substances

2011 Fiscal Year Final Research Report

• Project/Area Number: 21390533
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyoto University
• Principal Investigator: BESSHO Kazuhisa 京都大学, 医学研究科, 教授 (90229138)
• Co-Investigator(Kenkyū-buntansha): FUJIMURA Kazuma 京都大学, 医学研究科, 准教授 (30252399) ; OKUBO Yasunori 京都大学, 医学研究科, 講師 (50378618) ; SONOBE Junya 京都大学, 医学研究科, 講師 (50464219) ; KOYAMA Noriaki 京都大学, 医学研究科, 助教 (30599931) ; NAKAO Kazumasa 京都大学, 医学研究科, 医員 (40599932)
• Project Period (FY): 2009 – 2011
• Keywords: 軟骨 / C型ナトリウム利尿ペプチド / 骨形成 / 間葉系幹細胞 / アドレノメデュリン

Research Abstract

C-type natriuretic peptide(CNP) promotes endchondral bone growth by acting chondrocytes directly to increase the size of chondrocytes and the space extracellular space. In addition, CNP affects bone formation by acting osteoblasts directly.
Adult dental pulp and exfoliated deciduous teeth pulp-derived mesenchymal stem cells compared with bone marrow-derived mesenchymal stem cells, osteogenic differentiation potentials were high.
Adrenomedullin didn' t affect on osteodifferentiation.

Research Products

• [Journal Article] Innovative Collagen Nano-Hydroxyapatite Scaffolds Offer a Highly Efficient Non-Viral Gene Delivery Platform for Stem Cell-Mediated Bone Formation. (2012)
  • Author(s): C. M. Curtin, G. M. Cunniffe, F. G. Lyons, K. Bessho, G. R. Dickson, G. P. Duffy, F. J. O' Brien
  • Journal Title: Advanced Materials Volume: 24 Pages: 749-754
  • DOI: DOI:10.1002/adma.201103828
  • Peer Reviewed
• [Journal Article] Bessho:Id2 Controls Chondrogenesis Acting Downstream of BMP Signaling during Maxillary Morphogenesis (2012)
  • Author(s): T. Sakata-Goto, K. Takahashi, H. Kiso, B. Huang, H. Tukamoto, M. Takemoto, T. Hayashi, M. Sugai, T. Nakamura, Y. Yokota, A. Shimizu, H. C. Slavkin, K
  • Journal Title: Bone Volume: 50 Pages: 69-78
  • DOI: DOI:10.1016/j.bone.2011.09.049
  • Peer Reviewed
• [Journal Article] 筋突起に生じた骨軟骨種の1例 (2012)
  • Author(s): 高藤洋之、藤村和磨、高橋克、家森正志、大久保康則、別所和久
  • Journal Title: 日本口腔外科学会雑誌 Volume: 57 Pages: 25-29
  • Peer Reviewed
• [Journal Article] Bessho Experimental Study of Osteoinduction Using a New Material as a Carrier for Bone Morphogenetic Protein-2 (2011)
  • Author(s): N. Koyama, Y. Okubo, K. Nakao, K. Osawa, K.
  • Journal Title: Br J Oral Maxillofac Surg Volume: 49(4) Pages: 314-8
  • DOI: DOI:10.1016/j.bjoms.2010.05.010
  • Peer Reviewed
• [Journal Article] Pluripotency of Mesenchymal Cells Derived from Synovial Fluid in Patients with Temporomandibula Joint Disordar (2011)
  • Author(s): N. Koyama, Y. Okubo, K. Nakao, K. Osawa, K. Bessho
  • Journal Title: Life Science Volume: 89 Pages: 741-5
  • DOI: DOI:10.1016/j.lfs.2011.09.005
  • Peer Reviewed
• [Journal Article] Social Nicotine Dependence in Australian Dental Undergraduate Students (2011)
  • Author(s): B. Huang, K. Inagaki, C. Yoshii, M. Kano, P. V. Abbott, T. Noguchi, K. Takahashi, K. Bessho
  • Journal Title: International Dental Journal Volume: 61 Pages: 152-6
  • DOI: DOI:10.1111/j.1875-595X.2011.00032.x
  • Peer Reviewed
• [Journal Article] Impact of Different Surgery Modalities to Correct Class III Jaw Deformities on the Pharyngeal Airway Space (2011)
  • Author(s): T. F. Abdelrahman, K. Takahashi, K. Tamura, K. Nakao, K. M. Hassanein, A. Alsuity, H. Maher, K. Bessho
  • Journal Title: The Journal of Craniofacial Surgery Volume: 22 Pages: 1598-1601
  • DOI: DOI:10.1097/SCS.0b013e31822e5fc2
  • Peer Reviewed
• [Journal Article] Evaluation Tongue Sensary Disturbance by Somatosensory Evoked Magnetic Fields Following Tongue Stimulation (2011)
  • Author(s): H. Maezawa, K. Yoshida, M. Matsuhashi, Y. Yokoyama, T. Mima, K. Bessho, S. Fujita, T. Nagamine, H. Fukuyama
  • Journal Title: Neuroscience Research Volume: 71 Pages: 244-250
  • DOI: DOI:10.1016/j.neures.2011.07.1831
  • Peer Reviewed
• [Journal Article] Hypertension, Periodontal Disease, and Potassium Intake in Nonsmoking, Nondrinker African Women on No Medication (2011)
  • Author(s): M. Yamori, M. Njelekela, J. Mtabaji, Y. Yamori, K. Bessho
  • Journal Title: International Journal of Hypertension
  • DOI: DOI:10.4061/2011/695719
  • Peer Reviewed
• [Journal Article] Rigid Fixation of Intraoral Vertico-Sagittal Ramus Osteotomy for Mandibular Prognathism (2011)
  • Author(s): K. Fujimura, K. Bessho
  • Journal Title: J Oral Maxillofac Surg Volume: 70 Pages: 1170-3
  • DOI: DOI:10.1016/j.joms.2011.03.010
  • Peer Reviewed
• [Journal Article] Osteoinduction by Repeat Plasmid Injection of Human Bone Borphogenetic Protein-2 (2010)
  • Author(s): K. Osawa, Y. Okubo, K. Nakao, N. Koyama, K. Bessho
  • Journal Title: J Gene Med Volume: 12(12) Pages: 937-44
  • DOI: DOI:10.1002/jgm.1515
  • Peer Reviewed
• [Journal Article] BMP-2 Gene Transfer under Various Conditions with in vivo Electrop oration and Bone Induction
  • Author(s): H. Yamamoto, M. Kawai, N. Shiotsu, M. Watanabe, Y. Yoshida, M. Suzuki, H. Maruyama, J. Miyazaki, M. Ikegami, K. Bessho, T. Yamamot
  • Journal Title: Asian Jornal of Oral and Maxillofacial Surgery Volume: (IN PRESS)
  • Peer Reviewed
• [Presentation] Morphogenetical Analysis of Maxillary Deformity in Id2 KO mice (2011)
  • Author(s): T. Sakata-Goto, K. Takahashi, H. Kiso, B. Huang, H. Tukamoto, K. Bessho
  • Organizer: I. A. D. R
  • Place of Presentation: USA
  • Year and Date: 20110315-19
• [Presentation] Osteoinduction by Ultrasound-mediated Gene Transfer of Bone Morphogenetic Protein-2 (2011)
  • Author(s): K. Osawa, K. Nakao, N. Koyama, K. Bessho
  • Organizer: I. A. D. R
  • Place of Presentation: USA
  • Year and Date: 20110315-19
• [Presentation] 喫煙・ニコチンの歯槽骨再生治療に対する影響 (2011)
  • Author(s): 別所和久
  • Organizer: 第26回喫煙科学研究財団助成研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-22
• [Presentation] 再建から美容治療へ:歯科インプラントを利用した顎顔面矯正・再建手術と口腔顎顔面再生医療 (2011)
  • Author(s): 別所和久
  • Organizer: 香川県保険医協会医科歯科合同セミナー
  • Place of Presentation: 高松市
  • Year and Date: 2011-03-27
• [Presentation] 医療安全管理・歯科インプラントを利用した口腔外科手術 (2010)
  • Author(s): 別所和久
  • Organizer: 西京歯科医師会学術講演会
  • Place of Presentation: 京都市
  • Year and Date: 2010-12-04
• [Presentation] 抗血栓薬・ビスフォスフォネート製剤投与患者に対する歯科治療と歯科再生医療 (2010)
  • Author(s): 別所和久
  • Organizer: 第14回「空穂の会」学術講演会
  • Place of Presentation: 新潟市
  • Year and Date: 2010-11-13
• [Presentation] 再生医療の現状と歯科領域への応用 (2010)
  • Author(s): 別所和久
  • Organizer: 第40回日本口腔インプラント学会学術大会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-18
• [Presentation] 歯科インプラントを利用した口腔外科手術と歯科再生医療 (2010)
  • Author(s): 別所和久
  • Organizer: KIDS研究会学術講演会
  • Place of Presentation: 京都市
  • Year and Date: 2010-08-28
• [Presentation] ビスフォスフォネート製剤投与患者に発症する顎骨壊死・顎骨骨髄炎 (2010)
  • Author(s): 別所和久
  • Organizer: 久留米骨粗鬆症研究会
  • Place of Presentation: 久留米市
  • Year and Date: 2010-07-02
• [Presentation] 硬組織再生医療を目指して (2010)
  • Author(s): 別所和久
  • Organizer: 松本歯科大学学会特別講演会
  • Place of Presentation: 塩尻市
  • Year and Date: 2010-05-28
• [Presentation] ビスフォスフォネート製剤投与患者に対する歯科治療・歯科領域の再生医療の可能性 (2010)
  • Author(s): 別所和久
  • Organizer: 南口腔ケアセンター記念講演会
  • Place of Presentation: 京都市
  • Year and Date: 2010-04-24
• [Presentation] 抗血栓療法中患者の歯科治療・医療安全管理・歯科再生医療 (2010)
  • Author(s): 別所和久
  • Organizer: 福井県歯科医師学術講演
  • Place of Presentation: 福井市
  • Year and Date: 2010-04-18
• [Presentation] オーラルケア・マネジメントとオーラルヘルス・マネジメント (2010)
  • Author(s): 別所和久
  • Organizer: 京都西部化学療法研究会
  • Place of Presentation: 京都市
  • Year and Date: 2010-03-12
• [Book] Feasibility of BMP-2 Gene Therapy Using an Ultra-Fine Needle
  • Author(s): K. Osawa, Y. Okubo, K. Nakao, N. Koyama, K. Bessho
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/%7Eoral_surg/Oral_Surg_index.html
• [Patent(Industrial Property Rights)] 歯の切削方法及び装置 (2011)
  • Inventor(s): 別所和久、松久寛、高橋克、東郷由弥子
  • Industrial Property Rights Holder: 別所和久、松久寛、高橋克、東郷由弥子
  • Industrial Property Number: 特許、特願2011-060967
  • Filing Date: 2011-03-18