2011 Fiscal Year Final Research Report
Study of oncolytic virotherapy for oral cancer
| Project/Area Number |
21390536
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
YURA Yoshiaki 大阪大学, 大学院・歯学研究科, 教授 (00136277)
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| Co-Investigator(Renkei-kenkyūsha) |
IWAI Soichi 大阪大学, 歯学研究科, 助教 (10362675)
HAMADA Hamada 大阪大学, 歯学部附属病院, 医員 (80506361)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 腫瘍融解性ウイルス療法 / ヘルペスウイルス / 組換え体 / 細胞融合 / 遺伝子構造 |
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| Research Abstract |
Oncolytic virotherapy has been developed to destroy tumor cells by the cytopathic effect of virus vectors. In the present study, we produced a recombinant herpes simplex virus type 1(HSV-1) RH2 using R849 with LacZ gene at the deleted site of neurovirulentγ_1 34. 5 gene and strain HF with fusogenic ability. Nucleotide sequences of RH2 were determined with Genome Sequencer FLX. RH2 induced large syncytia in squamous cell carcinoma(SCC) cells and was deficient of neurovirulent gene. It can be used as a vector for the treatment of brain tumor as well as oral SCC
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