Molecular epidemiological study on cholangio-carcinogenesis induced by liver fluke Opisthorchis viverrini in Thailand.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21406019
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Hygiene
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: KAWANISHI Shosuke 鈴鹿医療科学大学, 薬学部, 教授 (10025637)
• Co-Investigator(Kenkyū-buntansha): MURATA Mariko 三重大学, 大学院・医学系研究科, 教授 (10171141) ; HIRAKU Yusuke 三重大学, 大学院・医学系研究科, 講師 (30324510) ; MA Nei 鈴鹿医療科学大学, 保健衛生学部, 教授 (30263015) ; OHNISHI Shiho 鈴鹿医療科学大学, 薬学部, 助手 (80511914) ; INOUE Sumiko 鈴鹿医療科学大学, 薬学部, 准教授 (20378657) ; CHINZEI Yasuo 鈴鹿医療科学大学, 医用工学部, 教授 (60024709)
• Co-Investigator(Renkei-kenkyūsha): OIKAWA Shinji 三重大学, 大学院・医学系研究科, 准教授 (10277006)
• Research Collaborator: YONGVANIT P. コンケーン大学, 教授 ; THANAN R. 鈴鹿医療科学大学, ポスト・ドクター
• Project Period (FY): 2009 – 2011
• Keywords: DNA損傷 / 感染 / 炎症 / がん予防 / 酸化ストレス / 胆管癌 / 肝吸虫

Research Abstract

Cholangiocarcinoma(CCA) is associated with chronic inflammation caused by liver fluke infection. Stem cell markers(CD133, OV6, CK-19) and DNA base damage(8-oxodG, 8-nitroguanine) were clearly colocalized in CCA. We identified 14 highly carbonylated proteins in CCA tissues. The oxidative modification of these proteins was significantly associated with poor prognoses as determined by the Kaplan-Meier method.

Research Products

• [Journal Article] Inflammation-induced protein carbonylation contributes to poor prognosis for cholangiocarcinoma (2012)
  • Author(s): Thanan R, Oikawa S, Yongvanit P, Hiraku Y, Ma N, Pinlaor S, Pairojkul C, Wongkham C, Sripa B, Khuntikeo N, Kawanishi S, Murata M
  • Journal Title: Free Radic Biol Med Volume: 52(8) Pages: 1465-72
  • DOI: DOI:10.1016/j.freeradbiomed.2012.01.018
  • Peer Reviewed
• [Journal Article] Role of nitrative and oxidative DNA damage in inflammation-related carcinogenesis (2012)
  • Author(s): Murata M, Thanan R, Ma N, Kawanishi S
  • Journal Title: J Biomed Biotechnol Volume: 2012 Pages: 623019
  • DOI: DOI:10.1155/2012/623019
  • Peer Reviewed
• [Journal Article] Nuclear Localization of COX-2 in relation to the Expression of Stemness Markers in Urinary Bladder Cancer (2012)
  • Author(s): Thanan R, Murata M, Ma N, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Oikawa S, Kawanishi S
  • Journal Title: Mediators Inflamm Volume: 2012 Pages: 165879
  • DOI: DOI:10.1155/2012/165879
  • Peer Reviewed
• [Journal Article] Nitrative DNA damage and Oct3/4 expression in urinary bladder cancer with Schistosoma haematobium infection (2011)
  • Author(s): Ma N, Thanan R, Kobayashi H, Hammam O, Wishahi M, El Leithy T, Hiraku Y, Amro el-K, Oikawa S, Ohnishi S, Murata M, Kawanishi S
  • Journal Title: Biochem Biophys Res Commun Volume: 414(2) Pages: 344-9
  • DOI: DOI:10.1016/j.bbrc.2011.09.073
  • Peer Reviewed
• [Journal Article] Nitrative and oxidative DNA damage caused by K-ras mutation in mice (2011)
  • Author(s): Ohnishi S, Saito H, Suzuki N, Ma N, Hiraku Y, Murata M, Kawanishi S
  • Journal Title: Biochem Biophys Res Commun Volume: 413(2) Pages: 236-40
  • DOI: DOI:10.1016/j.bbrc.2011.08.076
  • Peer Reviewed
• [Journal Article] Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic (2010)
  • Author(s): Ma N, Sasoh M, Kawanishi S, Sugiura H, Piao F
  • Journal Title: J Biomed Sci Volume: 17 Suppl 1 Pages: S7
  • DOI: DOI:10.1186/1423-0127-17-S1-S7
  • Peer Reviewed
• [Journal Article] The role of iNOS-mediated DNA damage in infection-and asbestos-induced carcinogenesis (2010)
  • Author(s): Hiraku Y, Kawanishi S, Ichinose T, Murata M
  • Journal Title: Ann N Y Acad Sci Volume: 1203 Pages: 15-22
  • DOI: DOI:10.1111/j.1749-6632.2010.05602.x
  • Peer Reviewed
• [Journal Article] Protective effect of taurine against nitrosative stress in the stomach of rat with water immersion restraint stress (2009)
  • Author(s): Ma N, Sasaki T, Sakata-Haga H, Ohta K, Gao M, Kawanishi S, Fukui Y
  • Journal Title: Adv Exp Med Biol Volume: 643 Pages: 273-83
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of 8-nitroguanine, a nitrative DNA lesion, in relation to inflammation-associated carcinogenesis (2009)
  • Author(s): Hiraku Y, Kawanishi S
  • Journal Title: Methods Mol Biol Volume: 512 Pages: 3-13
  • DOI: DOI:10.1007/978-1-60327-530-9_1
  • Peer Reviewed
• [Presentation] RRAD with tumor suppressive function is aberrantly methylated in nasopharyngeal carcinoma (2012)
  • Author(s): Mo Yingxi
  • Organizer: 第82回日本衛生学会総会
  • Place of Presentation: 京都
  • Year and Date: 20120325-26
• [Presentation] Protein carbonylation in liver fluke-induced cholangiocarcinoma in relation to poor prognosis (2012)
  • Author(s): Thanan Raynoo
  • Organizer: Mahidol International Conference on Infection and Cancer
  • Place of Presentation: タイ国バンコク市
  • Year and Date: 20120206-08
• [Presentation] Role of mutagenic 8-nitroguanine in estrogen-dependent radiation-induced mammary tumorigenesis of rats (2011)
  • Author(s): S. Kawanishi, N. Ma, M. Onoda, H. Inano, S. Ohnishi, M. Murata
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 20110800
• [Presentation] 寄生虫感染による幹様細胞における酸化・ニトロ化DNA損傷 (2011)
  • Author(s): Thanan Raynoo
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-05
• [Presentation] 上咽頭がんにおけるゲノムワイドなメチル化DNAの解析 (2011)
  • Author(s): Mo Yingxi
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-03
• [Presentation] K-ras activation and oxidative DNA damage (2009)
  • Author(s): S. Kawanishi, S. Ohnishi, H. Saito, N. Suzuki, Y. Hiraku, M. Murata
  • Organizer: 2009 Annual Meeting of the Society for Free Radical Research-Europe
  • Place of Presentation: Rome, Italy
  • Year and Date: 20090800
• [Book] Potential Biomarker to Evaluate the Risk of Inflammation-Related Carcinogenesis (2012)
  • Author(s): N. Ma, M. Murata, S. Ohnishi, R. Thanan, Y. Hiraku, S. Kawanishi
  • Total Pages: 201-224
  • Publisher: InTech
• [Book] Health and Lifestyle(K-ras activation and oxidative DNA damage) (2009)
  • Author(s): S. Kawanishi, S. Ohnishi
  • Total Pages: 75-78
• [Book] 炎症・再生医学事典 (2009)
  • Author(s): 川西正祐、大西志保
  • Total Pages: 328-331
  • Publisher: 朝倉書店