Analysis of the molecular mechanism of the repair of demyelination

2011 Fiscal Year Final Research Report

• Project/Area Number: 21500349
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagoya University
• Principal Investigator: SUGIMURA Yoshihisa 名古屋大学, 医学部附属病院, 講師 (50456670)
• Co-Investigator(Kenkyū-buntansha): OISO Yutaka 名古屋大学, 医学系研究科, 教授 (40203707)
• Co-Investigator(Renkei-kenkyūsha): SAWADA Makoto 名古屋大学, 環境医学研究所, 教授 (10187297)
• Project Period (FY): 2009 – 2011
• Keywords: グリア細胞 / レーザーマイクロダイセクション / 脱髄

Research Abstract

Osmotic demyelination syndrome(ODS) is a central demyelinating disease associated with a rapid correction of hyponatremia. To clarify the role of microglia in the pathogenesis of ODS, We used laser-capture microdissection to isolate single microglia and analyzed single-microglia gene-expression profiles using high-throughput microarray assays. Our data provide a new insight into the characteristic of microglia associated with injured oligodendrocytes and new molecular targets of minocycline treatment.

Research Products

• [Journal Article] Osteoclast Response to Low Extracellular Sodium and the Mechanism of Hyponatremia-induced Bone Loss (2011)
  • Author(s): Barsony J, Sugimura Y. Verbalis JG.
  • Journal Title: J Biol Chem Volume: 25 Pages: 10864-75
  • Peer Reviewed
• [Journal Article] Poly(A) tail length of neurohypophysial hormones is shortened under endoplasmic reticulum stress (2011)
  • Author(s): Morishita Y, Arima H, Hiroi M, Hayashi M, Hagiwara D, Asai N, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Takahashi M, Oiso Y
  • Journal Title: Endocrinology Volume: 152(12) Pages: 4846-55
  • Peer Reviewed
• [Journal Article] Time-dependent Changes in Proinflammatory and Neurotrophic Responses of Microglia and Astrocytes in a Rat Model of Osmotic Demyelination Syndrome (2011)
  • Author(s): Iwama S, Sugimura Y, Suzuki H, Suzuki H, Murase T, Ozaki N, Nagasaki H, Arima H, Murata Y, Sawada M, Oiso Y
  • Journal Title: GLIA Volume: 59 Pages: 452-62
  • Peer Reviewed
• [Journal Article] Minocycline prevents osmotic demyelination syndrome by inhibiting the activation of microglia (2010)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Ozaki N, Nagasaki H, Arima H, Sawada M, Oiso Y
  • Journal Title: J Am Soc Nephrol Volume: 21 Pages: 2090-8
  • Peer Reviewed
• [Journal Article] Hyponatremia-induced osteoporosis (2010)
  • Author(s): Verbalis JG, Barsony J, Sugimura Y, Tian Y, Adams D, J Carter EA, Resnick HE.
  • Journal Title: J Bone Miner Res Volume: 25 Pages: 554-63
  • Peer Reviewed
• [Journal Article] Activation of vasopressin neurons leads to phenotype progression in a mouse model for familial neurohypophysial diabetes insipid us (2010)
  • Author(s): Hiroi M, Morishita Y, Hayashi M, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Oiso Y, Arima H
  • Journal Title: J Physiol Regul Integr Comp Physiol Volume: 298 Pages: 486-93
  • Peer Reviewed
• [Journal Article] Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes (2009)
  • Author(s): Sugimura Y, Murase T, Oyama K, Uchida A, Sato N, Hayasaka S, Kano Y, Takagishi Y, Hayashi Y, Oiso Y, Murata Y
  • Journal Title: Diabetologia Volume: 52 Pages: 962-71
  • Peer Reviewed
• [Journal Article] Central adiponectin functions to in hibitarginine vasopressin release in consciousrats (2009)
  • Author(s): Iwama S, Sugimura Y, Murase T, Hiroi M, Goto M, Hayashi M, Arima H, Oiso Y
  • Journal Title: J Neuroendocrinol Volume: 21 Pages: 753-9
  • Peer Reviewed
• [Presentation] Pathophysiology of OsmoticDemyelination Syndrome (2011)
  • Author(s): Sugimura Y
  • Organizer: American Society of Nephrology, Kidney Week 2011(米国腎臓学会)招待講演
  • Place of Presentation: Philadelphia
  • Year and Date: 2011-11-10
• [Presentation] Minocycline Prevents Osmotic Demyelination Syndrome by Inhibiting the Activation of Microglia (2011)
  • Author(s): Sugimura Y
  • Organizer: 9th World Congress on Neurohypophysial Hormones(国際神経下垂体学会),招待講演
  • Place of Presentation: Boston
  • Year and Date: 2011-07-29
• [Presentation] COMPARATIVE ANALYSIS OF GENE EXPRESSION PROFILES OF MICROGLIA FROM DEMYELINATIVE LESIONS INOSMOTIC DEMYELINATION SYNDROME RAT MODEL (2010)
  • Author(s): Haruyuki Suzuki, YoshihisaSugimura, Shintaro Iwama, HiromiSuzuki, Atsushi Kiyota, MakotoSawada, Yutaka Oiso
  • Organizer: 10^<th> International Congress of Neuroimmunology
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-10-27
• [Presentation] Gene expression profiling of microglia from the demyelinative lesions in osmotic demyelination syndrome (2010)
  • Author(s): Sugimura Y, Suzuki H, Iwama S, Suzuki H, Kiyota A, Sawada M, Oiso Y
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] The pathogenes is of osmotic demyelination syndrome(ODS) and the therapy of ODS (2010)
  • Author(s): Sugimura Y
  • Organizer: 14^<th> International Congress of Endocrinology(ICE2010)(国際内分泌学会)招待講演
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-27
• [Presentation] The differing roles of nitric oxide synthase isoforms in osmotic demyelination syndrome inrats (2009)
  • Author(s): Sugimura Y, Murase T, Takefuji S, Takagi H, Suzuki H, Iwama S, Takagishi Y, Arima H, Sawada M, Oiso Y, Murata Y
  • Organizer: Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Presentation] Protective effects of minocycline on osmotic demyelination syndrome in rats (2009)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Arima H, Sawada M, OisoY
  • Organizer: Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Presentation] The differing pathological effects of nitric oxide synthase is forms in osmotic demyelination syndrome in rats (2009)
  • Author(s): Sugimura Y, Murase T, Takefuji S, Takagi H, Iwama S, Suzuki H, Takagishi Y, Arima H, Sawada M, Oiso Y, Murata Y
  • Organizer: The 32nd ANNUAL MEETING OF THEJAPAN NEUROSCIENCE SOCIETY
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Proinflammatory and Neurotrophic Responses of Microglia and Astrocytes in a RatModel for Osmotic Demyelination Syndrome (2009)
  • Author(s): Iwama S, Sugimura Y, Suzuki H, Suzuki H, Arima H, Murata Y, Sawada M, Oiso Y
  • Organizer: 8th World Congress on Neurohypoohysial Hormones
  • Place of Presentation: Kitakyushu
  • Year and Date: 2009-09-07
• [Presentation] Minocycline prevents osmotic demyelination by inhibiting the activation of microglia in rats (2009)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Arima H, Sawada M, Oiso Y
  • Organizer: 8th World Congress on Neurohypoohysial Hormones
  • Place of Presentation: Kitakyushu
  • Year and Date: 2009-09-07
• [Presentation] 浸透圧性脱髄症候群ラットモデルにおけるミノサイクリンの検討 (2009)
  • Author(s): 鈴木陽之,椙村益久,岩間信太郎,有馬寛,大磯ユタカ
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬
  • Year and Date: 2009-04-23
• [Book] ADH(AVP)不適切分泌症候群、症候群ハンドブック403 (2011)
  • Author(s): 椙村益久、大磯ユタカ
  • Total Pages: 403
  • Publisher: 中山書店
• [Book] 高ナトリウム血症内分泌・糖尿病科 (2009)
  • Author(s): 椙村益久、大磯ユタカ
  • Total Pages: 393-400
  • Publisher: 科学評論社