Generation of knockout mice and transgenic mice of the Alivin family

2012 Fiscal Year Final Research Report

• Project/Area Number: 21500396
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: 公益財団法人東京都医学総合研究所 (2012); Tokyo Metropolitan Organization for Medical Research (2009-2011)
• Principal Investigator: ONO Tomio 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (60231239)
• Co-Investigator(Kenkyū-buntansha): SHITARA Hiroshi 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (90321885) ; MATSUOKA Kunie 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (40291158) ; TAYA Choji 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 室長 (90175456)
• Project Period (FY): 2009 – 2012
• Keywords: ノックアウトマウス / アポトーシス / 神経細胞死 / ロイシンリッチリピート / 免疫グログリン / 神経活動

Research Abstract

In order to study biological functions of the Alivin family, we have generated mutant stains of mice harboring Alivin1 knockout (KO) allele, Alivin2 KO allele, Alivin3 KO allele, and an Alivin1 over-expressing transgene. Results of open-field tests suggest that the transgenic mice over-expressing ALIVIN1 have enhanced anxiety. The fibroblast prepared from embryos of the Alivin1 KO mice showed reduced migratory activity compared with that of wild type.

Research Products

• [Journal Article] Comprehensive application of an mtDsRed2-Tg mouse strain for mitochondrial imaging (2012)
  • Author(s): Yamaguchi J, Nishiyama S, Shimanuki M, Ono T,Sato A, Nakada K, Hayashi J, Yonekawa H, Shitara H
  • Journal Title: Transgenic Res 21 Pages: 439-47
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21792696
  • Peer Reviewed
• [Journal Article] Over-expression of Tfam improves the mitochondrial disease phenotypes in a mouse model system (2010)
  • Author(s): Nishiyama S, Shitara H, Nakada K, Ono T, Sato A, Suzuki H, Ogawa T,Masaki H, Hayashi J, Yonekawa H
  • Journal Title: Biochem Biophys Res Commun Volume: 401 (1) Pages: 26-31
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20816751
  • Peer Reviewed
• [Journal Article] Mice deficient in alivin1/amigo2 show enhanced locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa H
  • Journal Title: Neurosci Res Volume: 65 Pages: S228- S228
• [Presentation] 血管新生におけるCK2とポリアミン の関与 (2012)
  • Author(s): 芦野洋美, 小野富男, 入江敦, 島村眞里子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20121214-20121216
• [Presentation] A new spontaneous mouse mutant of the kyphoscoliosis peptidase gene, a muscular dystrophy gene (2011)
  • Author(s): Tomio Ono, Toyoyuki Takada, Kazuhiko Watabe, Hiroshi Shitara, Rie Ishii, Toshihiko Shiroishi , Choji Taya, and Hiromichi Yonekawa
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] CK2阻害物質は血管新生を抑制する (2011)
  • Author(s): 芦野洋美, 小野富男, 入江敦, 島村眞里子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] IkBキナーゼ阻害剤によるin vivo 血管新生の抑制 (2010)
  • Author(s): 芦野洋美, 小野富男, 入江敦, 山本行男, 島村眞里子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] Enhanced locomotor activity in alivin1/amigo2 deficient mice (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa H
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] Inhibitory effect of N-acetyl-L-cysteine on angiogenesis in vivo (2009)
  • Author(s): Ashino,H., Ono,T., Yamamoto,Y., Shimamura,M
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] Deficiency of alivin1/amigo2 in mice enhances locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa, H
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Presentation] Mice deficient in alivin1/amigo2 show enhanced locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa H
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090916-18