Development of a novel healing system for bone disease using a synergetic effect of magnetic fields and bone-regulating hormone

2011 Fiscal Year Final Research Report

• Project/Area Number: 21500404
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kanazawa University
• Principal Investigator: SUZUKI Nobuo 金沢大学, 環日本海域環境研究センター, 准教授 (60242476)
• Co-Investigator(Kenkyū-buntansha): YAMADA Sotoshi 金沢大学, 環日本海域環境研究センター, 教授 (80019786) ; TABUCHI Yoshiaki 富山大学, 生命科学先端研究センター, 准教授 (20322109) ; WADA Shigehito 富山大学, 附属病院, 講師 (50303219)
• Project Period (FY): 2009 – 2011
• Keywords: 骨芽細胞 / 破骨細胞 / 極低周波磁場 / ホルモンとの相互作用 / ウロコ / ラットの頭蓋骨 / メラトニン

Research Abstract

To develop a novel healing system for bone disease using magnetic fields, we examined the effects of extremely low-frequency(ELF) magnetic fields(60Hz) on osteoblasts and osteoclasts of goldfish scales using in vitro assay systems. The osteoblasts and osteoclasts in goldfish scales responded sensitively to the ELF magnetic fields in an in vitro experiment. The results agreed with those of rat calvarial osteoblasts and osteoclasts. In addition, the synergetic effect of magnetic fields and a bone-regulating hormone, melatonin, was confirmed. Thus, we succeeded in creating a novel healing system for bone disease by magnetic fields.

Research Products

• [Journal Article] Expression of osteoblast-specific genes during spontaneous goldfish scale regeneration and role of cell-to-cell contact in controlling the onset of resorption/regeneration process found in intra-scalepocket autotransplantation of modified scales (2012)
  • Author(s): Thamamongood, T. A, Furuya, R., Fukuba, S., Nakamura, M., Suzuki, N. andHattori, A
  • Journal Title: Bone Pages: 1240-1249
  • Peer Reviewed
• [Journal Article] Cloning of two members of the calcitonin receptor family from stingray (2012)
  • Author(s): Suzuki, N., Sekiguchi, T., Satake, H., Kato, K., Nishiyama, Y., Takahashi, H., Danks, J. A., Martin, T. J., Hattori, A., Nakano, M., Kakikawa, M., Yamada, S., (他8名)
  • Journal Title: Gene Volume: 499 Pages: 90-95
  • Peer Reviewed
• [Journal Article] Parathyroid hormone 1(1-34) acts on the scales and involves calcium metabolism in goldfish (2011)
  • Author(s): Suzuki, N., (他19名)
  • Journal Title: Bone Volume: 48 Pages: 1186-1193
  • Peer Reviewed
• [Journal Article] 自動面積測定法によるキンギョのウロコを骨のモデルとしたアッセイ法の改良 (2011)
  • Author(s): 北村敬一郎, 中野淳, 川部季美, 早川和一, 根本鉄, 大嶋雄治, 島崎洋平, 服部淳彦, 鈴木信雄
  • Journal Title: 日本海域研究 Volume: 42 Pages: 27-34
  • Peer Reviewed
• [Journal Article] Osteoblas tactivity in the goldfish scale responds sensitively to mechanical stress (2010)
  • Author(s): Kitamura, K., Suzuki, N., Sato, Y., Nemoto, T., Ikegame, M., Yamamoto, T., Shimizu, N., Kondo, T., Furusawa, Y., Wada, S.
  • Journal Title: Comp. Biochem. Physiol. Volume: 156 Pages: 357-363
  • Peer Reviewed
• [Journal Article] Diurnal expressions of four subtypesof melatonin receptor genes in the optictectum and retina of goldfish (2009)
  • Author(s): Ikegami, T., Azuma, K., Nakamura, M., Suzuki, N., Hattori, A. and Ando, H
  • Journal Title: Comp. Biochem. Physiol. Volume: 152 Pages: 219-224
  • Peer Reviewed
• [Presentation] Activation of osteoclasts in female goldfish during the reproductive stage (2012)
  • Author(s): Maruyama, Y., Suzuki, N., Hattori, A
  • Organizer: 7^<th> Congress of the Asia and Oceania Society for Comparative Endocrinology
  • Place of Presentation: Sunway Resort Hotel(Malaysia)
  • Year and Date: 20120303-07
• [Presentation] キンギョの再生ウロコのin vivoおよびin vitro系による機械的刺激の骨代謝への影響 (2011)
  • Author(s): 佐藤雄亮,根本鉄,鈴木信雄,矢野幸子,服部淳彦,北村敬一郎
  • Organizer: 平成23年度生体医工学会北陸支部大会
  • Place of Presentation: 金沢大学サテライトプラザ(石川県)
  • Year and Date: 2011-12-10
• [Presentation] 赤外・ラマン分光によるキンギョのウロコの状態分析 (2011)
  • Author(s): 奈良雅之,服部淳彦,大西晃宏,赤塚陽子,鈴木信雄,松田准一
  • Organizer: 医用分光学研究会
  • Place of Presentation: 島根大学(島根県)
  • Year and Date: 2011-11-13
• [Presentation] 魚類のウロコにおけるホルモン及び物理的刺激(磁場刺激)の応答 (2011)
  • Author(s): 鈴木信雄
  • Organizer: 日本歯科基礎医学会サテライトシンポジウム
  • Place of Presentation: 朝日大学(岐阜県)
  • Year and Date: 2011-09-30
• [Presentation] 骨とメラトニン (2011)
  • Author(s): 服部淳彦,池亀美華,矢野幸子,鈴木信雄
  • Organizer: 第3回抗加齢内分泌研究会学術集会
  • Place of Presentation: 鶴見大学会館(神奈川県)
  • Year and Date: 2011-09-04
• [Presentation] 繁殖期の雌キンギョにおけるカルシウム代謝に対するメラトニンの作用 (2010)
  • Author(s): 丸山雄介,鈴木信雄,伊藤正則,服部淳彦
  • Organizer: 第81回日本動物学会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 20100923-25
• [Presentation] 交流磁場の骨形成促進作用:再生ウロコを用いたinvivoの解析 (2010)
  • Author(s): 鈴木信雄,柿川真紀子,山田外史,田渕圭章,高崎一朗,古澤之裕,近藤隆,和田重人,廣田憲之,北村敬一郎,岩坂正和,服部淳彦,上野照剛
  • Organizer: 平成22年度宇宙生物科学会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 20100917-18
• [Presentation] 磁場による骨形成作用:ウロコを用いた解析 (2010)
  • Author(s): 鈴木信雄
  • Organizer: バイオサイエンスシンポジウム
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2010-02-24
• [Presentation] Development andapplication of a fish scale in vitroassay system : Fish scale is a suitablemodel for analysis of bone metabolism (2009)
  • Author(s): Suzuki, N
  • Organizer: 2009 Korea-Japan Joint Research Project Symposium."Aging, Radiation and Environment"
  • Place of Presentation: Pusan National University(Busan, Korea)
  • Year and Date: 2009-12-16
• [Presentation] 魚のウロコをモデル系とした磁場の骨代謝に対する作用 (2009)
  • Author(s): 鈴木信雄
  • Organizer: 日本磁気科学会年会サテライトシンポジウム
  • Place of Presentation: 信州大学(長野県)
  • Year and Date: 2009-11-12
• [Presentation] 交流磁場の骨代謝に対する作用:魚鱗を用いたモデル系による解析 (2009)
  • Author(s): 鈴木信雄,柿川真紀子,山田外史,田渕圭章,高崎一朗,古澤之裕,近藤隆,和田重人,廣田憲之,北村敬一郎,岩坂正和,服部淳彦,上野照剛
  • Organizer: 第34回日本比較内分泌学会大会
  • Place of Presentation: 大阪府
  • Year and Date: 2009-10-23
• [Presentation] 魚類のカルシウム代謝におけるウロコの生理学的役割:副甲状腺ホルモンに対する作用 (2009)
  • Author(s): 鈴木信雄
  • Organizer: 宇宙生物科学会第23回大会
  • Place of Presentation: 筑波宇宙センター(茨城県)
  • Year and Date: 2009-10-02
• [Book] Comparative and general aspects of calcium homeostasis and its hormonal regulations. In "Evolution of calcium homeostasis and its hormonal regulation in vertebrates".Virtual special issues in Zoological Science
  • Author(s): Suzuki, N. and Sakamoto, T
• [Book] 第3章親子判別『身近な動物を使った実験4』
  • Author(s): 服部淳彦,田畑純,鈴木信雄
  • Publisher: 三共出版