2011 Fiscal Year Final Research Report
Experimental design of ELISA for quantitative MCT4 analysis and an investigation of signal transduction cascades
| Project/Area Number |
21500690
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
IIZUKA Kenji 北海道医療大学, 薬学部, 准教授 (10344467)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAFUJI Masahiko 北海道医療大学, 薬学部, 教授 (20142987)
MACHIDA Takuji 北海道医療大学, 薬学部, 講師 (90433424)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生活習慣病 |
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| Research Abstract |
Two types of antibodies, anti-monocarboxylate transporter(MCT) 4 polyclonal antibody recognizes C-terminal end of MCT4 and the anti-MCT4 polyclonal antibody created by considering synthetic peptide of the intermediate part of MCT4 molecule as an antigen, were prepared. Using these antibodies, we constructed both a single and a sandwiche enzyme-linked immunosorbent assay system for quantification of MCT4 concentration in serum and/or culture medium.
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