2011 Fiscal Year Final Research Report
Development of glycosyl modification machinery by sequential multiple infections of baculoviruses
| Project/Area Number |
21510220
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Akita University |
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Principal Investigator |
GOTOH Takeshi 秋田大学, 大学院・工学資源学研究科, 教授 (10215494)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生物活性分子の設計 / 合成 / 糖鎖化学 |
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| Research Abstract |
Several recombinant baculoviruses that contained c DNAs of glycosyltransferase soluble domains under the control of gp64 early promoter were constructed, in order to develop a glycosyl modification machinery in insect cells, in which multiple glycosyltransferases were sequentially expressed. In addition, a novel method was proposed that the proteolytic degradation of recombinant proteins expressed in baculovirus-infected insect cells was efficiently inhibited.
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Research Products
(15 results)
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[Presentation] Expression of recombinant human (rh)-renin in Sf-9 insect cells : Application of rh-renin for inhibitor screening2009
Author(s)
Takahashi, S., Hori, K., Hiwatashi, K., Awa, H., Kikuchi, K.-I., Gotoh, T
Organizer
6th General Meeting of the International Proteolysis Society
Place of Presentation
Marriot Hotel (Surfers Paradise, QLD Australia)
Year and Date
20091026-30
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[Presentation] Characterization of prorenin processing enzyme responsible for in situ active human renin production by baculovirus-infected Sf-9 cells and inhibition of excessive processing2009
Author(s)
Gotoh, T., Awa, H., Hikage, S., Kikuchi, K.-I., Takahashi, S
Organizer
14th European Congress on Biotechnology
Place of Presentation
Palau de Congressos de Catalunya (Barcelona, Spain)
Year and Date
20090913-16
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