2011 Fiscal Year Final Research Report
Measurement of Oxidative Stress on a single cell level by the beam deflection method and capillary electrophoresis
| Project/Area Number |
21550076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Fukuoka Institute of Technology (2010-2011)
University of Fukui (2009) |
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Principal Investigator |
WU Xing-zheng 福岡工業大学, 工学部, 教授 (70234961)
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| Co-Investigator(Kenkyū-buntansha) |
TERADA Satoshi 福井大学, 大学院・工学研究科, 准教授 (60311685)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 計測 / 細胞 / 酸化ストレス / 活性酸素 / プローブ光の偏向 / 電気泳動 / 化学発光 |
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| Research Abstract |
This research aimed at developing a novel method for measurement of oxidative stress in a single cell. Firstly, behavior of a single cell before and after an oxidative stress such as by addition of a hydrogen peroxide solution was monitored and compared by the deflection method of a probe beam. Then, analysis of active oxygen species in cells was carried out by a home-made capillary electrophoresis-laser induced fluorescence detection system. The luminol-chemliuminescence method was further used for quantitative analysis of active oxygen species in cells. HeG2 cell was cultured and used as a model cell sample.
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