2011 Fiscal Year Final Research Report
Development of sensitive analytical method for protein and nucleic acid using natural elemental tags
| Project/Area Number |
21550094
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
TAKATSU Akiko 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究科長 (10357361)
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| Co-Investigator(Kenkyū-buntansha) |
INAGAKI Kazumi 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究室付 (50356490)
FUJII Shin-ichiro 独立行政法人産業技術総合研究所, 計測標準研究部門, 研究員 (10415739)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生体分析 / 核酸 / タンパク質 / 誘導結合プラズマ質量分析 / 液体クロマトグラフィー / キャピラリー電気泳動 |
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| Research Abstract |
A high performance interface device was developed for sample introduction to inductively coupled plasma mass spectrometer(ICP-MS) at low flow rate. Using this interface device, hyphenation of capillary liquid chromatography(LC) or capillary electrophoresis with ICP-MS was achieved. DNA molecules separated with CE or capillary LC were sensitively and selectively detected with ICP-MS in quantification of phosphorus as an elemental tag. We have concluded that this system can provide a fast screening method for various bio-molecules in biological samples.
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