Action of antimicrobial peptides into the bacterial outer membrane, activity enhancement, and the technology for single molecular evaluation

2011 Fiscal Year Final Research Report

• Project/Area Number: 21550168
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: FUKUOKA Satoshi 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (40357885)
• Co-Investigator(Kenkyū-buntansha): ITO Tamitake 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (00351742)
• Project Period (FY): 2009 – 2011
• Keywords: 抗菌ペプチド / リポ多糖 / リピドA / 胆汁酸 / 表面増強ラマン分光 / 単一分子解析 / 高次構造 / 生物活性

Research Abstract

The interaction of lipopolysaccharide(LPS) and its hydrophobic part lipid A with antimicrobial peptides or bile acids was studied. We elucidated the conformation of their phosphate groups due to peptide binding, and the profile of the secondary structure of the peptides by physicochemical analyses. The thermodynamics of binding between peptides and lipid A showed that the acidic amino acid substituted peptides improved the binding activity. The conformation change of the peptide enhanced the ability of incorporation into the lipid A aggregates, along with changes in biochemical and biophysical parameters. The surface enhanced Raman scattering spectroscopy study indicated the applicability of that to the LPS evaluation.

Research Products

• [Journal Article] Biophysical investigations into the interactions of endotoxins with bile acids (2012)
  • Author(s): Satoshi Fukuoka, Walter Richter, Jorg Howe, Jorg Andra, Manfred Rossle, Christian Alexander, Thomas Gutsmann and Klaus Brandenburg
  • Journal Title: Innate Immunity Volume: Vol.18 Pages: 301-317
  • DOI: DOI:10.1177/1753425911404093
  • Peer Reviewed
• [Journal Article] Molecular basis for endotoxin neutralization by amphipathic peptides derived from the α-helical cationic core-region of NK-lysin (2010)
  • Author(s): Klaus Brandenburg, Patrick Garidel, Satoshi Fukuoka, Jorg Howe, Michel H. J. Koch, Thomas Gutsmann, and Jorg Andra
  • Journal Title: Biophysical Chemistry Volume: Vol.150 Pages: 80-87
  • DOI: DOI:10.1016/j.bpc.2010.01.009
  • Peer Reviewed
• [Presentation] リポ多糖の膜構造や生物活性を変化させる生体関連分子 (2012)
  • Author(s): 福岡聰, Walter Richter, Jorg Howe, Jorg Andra, Manfred Rossle, Christian Alexander, Thomas Gutsmann, and Klaus Brandenburg
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 神奈川県・慶応義塾大学
  • Year and Date: 2012-03-25
• [Presentation] 金属ナノ構造体とプラズモン共鳴の解析に基づくSERS電磁理論の定量的検証 (2011)
  • Author(s): 伊藤民武
  • Organizer: 北海道大学電子研講演会
  • Place of Presentation: 北海道・北海道大学
  • Year and Date: 2011-04-22
• [Presentation] 単一ナノ粒子分光を用いた表面増強ラマン散乱メカニズムの解明と生体サンプルへの応用 (2011)
  • Author(s): 伊藤民武
  • Organizer: 岡山理科大学工学部バイオ・応用化学科セミナー
  • Place of Presentation: 岡山県・岡山理科大学
  • Year and Date: 2011-01-19
• [Presentation] Incorporation of bile acids into Erwinia carotovora lipid A aggregates and influence on their biological activity and phase behaviors (2010)
  • Author(s): Satoshi Fukuoka, Walter Richter, Jorg Howe, Jorg Andra, Manfred Rossle, Christian Alexander, Thomas Gutsmann, and Klaus Brandenburg
  • Organizer: International Endotoxin and Innate Immunity Society Conference(IEIIS2010)
  • Place of Presentation: カナダ・バンクーバー・フェアモントホテル
  • Year and Date: 2010-10-08
• [Presentation] 単一大腸菌表面に光還元合成した銀ナノ粒子からの表面増強ラマン散乱 (2010)
  • Author(s): 北濱康孝,伊藤民武,石堂智美,平野研,石川満
  • Organizer: 第71回応用物理学会学術講演会
  • Place of Presentation: 長崎県・長崎大学
  • Year and Date: 2010-09-16
• [Presentation] 表面増強ラマン散乱分光による疾病関連分子や細胞表面タンパク質分子の超高感度識別 (2010)
  • Author(s): 伊藤民武
  • Organizer: 第2回環境・生体の関わる物理・化学の研究会
  • Place of Presentation: 沖縄県・琉球大学
  • Year and Date: 2010-06-03
• [Presentation] アミノ酸置換抗菌ペプチドの細菌外膜への結合と抗菌活性 (2010)
  • Author(s): 福岡聰, Jorg Howe, Jorg Andra, Thomas Gutsmann, and Klaus Brandenburg
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪府・近畿大学
  • Year and Date: 2010-03-28
• [Presentation] エンドトキシンの両親媒性ペプチドによる活性中和の分子機構 (2010)
  • Author(s): Klaus Brandenburg, Patrick Garidel, Satoshi Fukuoka, Jorg Howe, Michel H. J. Koch, Thomas Gutsmann, and Jorg Andra
  • Organizer: 平成21年度産技連LsBt合同発表会
  • Place of Presentation: 茨城県・産業技術総合研究所
  • Year and Date: 2010-02-04