2011 Fiscal Year Final Research Report
Monitoring of cell membrane disruption and recovery by broadband dielectric spectroscopy
| Project/Area Number |
21560447
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Measurement engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
ASAMI Koji 京都大学, 化学研究所, 准教授 (90127936)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 計測システム / 誘電分光 / 細胞膜 / 赤血球 / 界面分極 / 溶血 |
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| Research Abstract |
A suspension of erythrocyte ghosts obtained by hypotonic hemolysis shows dielectric dispersion termedα-dispersion below 10 kHz. The exact nature of theα-dispersion, however, has not been studied because of difficulty in low-frequency measurement due to electrode polarization, and therefore its origin has not been clearly understood. In this study, a new electrode configuration was designed to extend the available frequency region to a lower frequency side, making it possible to investigate theα-dispersion in detail. The occurrence of theα-dispersion correlated strongly with the presence of holes in the ghost membranes and its properties were exactly interpreted by the numerical simulation with the spherical membrane model with a hole. The numerical simulation provided a liner relation between the characteristic frequency of theα-dispersion and the hole radius, and thereby the hole radius being determined straightforwardly.
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