2011 Fiscal Year Final Research Report
Fusion of affinity techniques for protein concentration and novel proteome analysis
| Project/Area Number |
21560810
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUMADA Youichi 京都工芸繊維大学, 工芸科学研究科, 助教 (70452373)
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| Co-Investigator(Renkei-kenkyūsha) |
NOMURA Akinari 京都大学, 医学研究科, 助教 (20402901)
IWATA Hiroo 京都大学, 再生医科学研究所, 教授 (30160120)
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| Project Period (FY) |
2009 – 2011
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| Keywords | アフィニティー濃縮 / プロテオーム解析 / ペプチド合成 / 医療診断 / 抗体医薬 |
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| Research Abstract |
For the detection of minor protein or small amout of protein, which are vey useful for cancer and the other adult deseases, several approach is proposed using the peptide affinity technology for concentration of the minor protein by washing out the major protein. For the evaluation and promotion of these technology, we tried to develop the treatment of high concentration of standard protein and the graphic analysis method of the 2-DE gel image. These technolgy were applied for the scFv production to identify the culture phase and screening of proteins, which attached specifically to the surface of plastics.
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