Silencing mechanisms of plant heterochromatin regions

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: HABU Yoshiki 独立行政法人農業生物資源研究所, ゲノム機能改変研究ユニット, 主任研究員 (80266915)
• Project Period (FY): 2009 – 2011
• Keywords: サイレンシング / 反復配列 / シトシンメチル化 / シロイヌナズナ / MORPHEUS' MOLECULE1

Research Abstract

We analyzed function of factors involved in silencing of endogenous repetitive elements in the plant genome. The results suggested the presence of three independent silencing pathways regulated by(1) MORPHEUS' MOLECULE1(MOM1),(2) RNA-directed DNA methylation(RdDM), and(3) both of MOM1 and RdDM, respectively. Chromodomain is often found in MOM1 protein of plants other than Arabidopsis thaliana, but our analysis indicated that it is dispensable for silencing activity of MOM1.

Research Products

• [Journal Article] Tandemly arranged chalcone synthase A genes contribute to the spatially regulated expression of siRNA and the natural bicolor phenotype in Petunia hybrida (2012)
  • Author(s): Morita Y, Saito R, Ban Y, Tanikawa N, Kuchitsu K, Ando T, Yoshikawa Ma, Habu Y, Ozeki Y, Nakayama M
  • Journal Title: Plant J Volume: 70 Pages: 739-749
  • Peer Reviewed
• [Journal Article] siRNA-mediated chromatin maintenance and its function in Arabidopsis thaliana (2011)
  • Author(s): Kanno T, Habu Y
  • Journal Title: Biochim Biophys Acta-Gene Regulatory Mechanisms Volume: 1809 Pages: 444-451
  • Peer Reviewed
• [Journal Article] 植物におけるRNAサイレンシング機構:転写後型ジーンサイレンシング(PTGS)と転写型ジーンサイレンシング(TGS) (2011)
  • Author(s): 吉川学,菅野達夫,土生芳樹
  • Journal Title: 細胞工学 Pages: 706-711
• [Journal Article] Transduction of RNA-directed DNA methylation signals to repressive histone marks in Arabidopsis thaliana (2010)
  • Author(s): Numa H, Kim J-M, Matsui A, Kurihara Y, Morosawa T, Ishida J, Mochizuki Y, Kimura H, Shinozaki K, Toyoda T, Seki M, Yoshikawa M, Habu Y
  • Journal Title: EMBO J Volume: 29 Pages: 352-362
  • Peer Reviewed
• [Journal Article] Locus-specific dependency of endogenous silent loci on MOM1 and non-CG methylation in Arabidopsis thaliana (2010)
  • Author(s): Habu Y, Yoshikawa M
  • Journal Title: Plant Signal Behav Volume: 5 Pages: 724-726
  • Peer Reviewed
• [Journal Article] Epigenetic silencing of endogenous repetitive sequences by MORPHEUS' MOLECULE1 in Arabidopsis thaliana (2010)
  • Author(s): Habu Y
  • Journal Title: Epigenetics Volume: 5 Pages: 562-565
• [Presentation] クロマチン修飾を介した植物のゲノム組成改変 (2011)
  • Author(s): 土生芳樹
  • Organizer: 第17回京都府立大学植物バイテクシンポジウム「エピゲノム研究の展開と植物科学」
  • Year and Date: 20110000
• [Presentation] 育種におけるクロマチン修飾:減数分裂期組み換えとのかかわり日本育種学会シンポジウム「RNAサイレンシング研究の新展開とその育種的利用に向けた展望」 (2009)
  • Author(s): 土生芳樹
  • Organizer: 日本育種学会2009年秋季大会
  • Year and Date: 20090000
• [Presentation] ゲノムタイリングアレイが拓くヘテロクロマチンサイレンシングの新局面日本植物生理学会シンポジウム「植物科学におけるタイリングアレイと次世代シーケンサーの利用」 (2009)
  • Author(s): 土生芳樹
  • Organizer: 第50回日本植物生理学会年会
  • Year and Date: 20090000
• [Book] Mechanisms linking cytosine methylation to histone modification in Arabidopsis thaliana. In : Noncoding RNAs in plants (2011)
  • Author(s): Arita K, Kanno T, Yoshikawa M, Habu Y
  • Total Pages: 237-249
  • Publisher: Springer
• [Remarks]
  • URL: http://www.nias.affrc.go.jp/press/20100121/