2011 Fiscal Year Final Research Report
Function of a novel gene OsCEO containing a TPR motif
| Project/Area Number |
21570050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SASAKI Tadamasa 東京理科大学, 基礎工学部・生物工学科, 助教 (50432802)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 植物分子機能 |
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| Research Abstract |
An endosperm mutant flo2 is represented by reduced production of seed storage starch and storage proteins, and results in small chalky gains. This suggests that the gene responsible to this mutation encodes a regulatory gene, which may globally control the biosynthesis of storage substances. We isolated the gene responsible to the flo2 mutation by a map-based cloning method. This gene, named FLO2, contained a TPR motif, in which a point mutation occurred in the mutant gene. The wild-type FLO2 gene completely restored the phenotype of the flo2 mutation, indicating that this gene was the gene responsible to the flo2 mutation. The expression of FLO2 gene was detected in leaves and immature seeds. We found that the FLO2 protein interacted with a bHLH transcription factor and a LEA protein, suggesting that this protein may be involved in regulation of the gene expression for production of the storage substances. Overexpression of the FLO2 gene indicated overexpression of the genes for storage proteins and storage starch, resulting in enlargement of the grain size.
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Research Products
(28 results)
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[Journal Article] A novel factor FLOURY ENDOSPERM 2 is involved in regulation of rice grain size and starch quality2010
Author(s)
K.-C. She, H. Kusano, K. Koizumi, H. Yamakawa, M. Hakata, T. Imamura, M. Fukuda, N. Naito, Y. Tsurumaki, M. Yaeshima, T. Tsuge, K. Matsumoto, M. Kudoh, E. Itoh, S. Kikuchi, N. Kishimoto, J. Yazaki, T. Ando, M. Yano, T. Aoyama, T. Sasaki, H. Satoh, H. Shimada
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Journal Title
Plant Cell
Volume: 22
Pages: 3280-3294
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[Presentation] A novel factor FLOURY ENDOSPERM 2 is involved in regulation of rice grain quality2010
Author(s)
K.-C. She, H. Kusano, H. Yamakawa, M. Hakata, Y. Tsurumaki, M. Yaeshima, T. Ando, M. Yano, T. Aoyama, T. Sasaki, H. Satoh, H. Shimada
Organizer
International Symposium on Biodiversity Science 2010
Place of Presentation
Nagoya
Year and Date
20100731-0803
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