2011 Fiscal Year Final Research Report

Elucidation of flavone C-glycosides biosynthesis pathway in rice based on transcriptome analysis

Research Project

Project/Area Number	21570057
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plant molecular biology/Plant physiology
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	SAKAKIBARA Keiko 独立行政法人理化学研究所, メタボローム機能研究グループ, 上級研究員 (20360555)
Project Period (FY)	2009 – 2011
Keywords	C-配糖化 / フラボノイド / トランスクリプトミクス / 二次代謝 / イネ / 植物
Research Abstract	Transcriptome coexpression analysis based on correlation coefficients database developed independently showed that the expression patterns of genes in flavonoid C-glycoside biosynthetic pathway are correlated with genes encoding chalcone synthase and chalcone isomerase and suggests some genes as candidates involved in flavonoid C-glycoside biosynthesis. By flavonoid analyses using several rice organs, 29 flavonoid-related peaks were detected and the structures of 11 peaks were supposed. Additional flavonoid related peaks were detected in some rice FOX lines. The functions of candidate genes were assayed by measurement of enzymatic activity using recombinant proteins and overexpression in Arabidopsis in addition to molecular genetic analysis.

(4 results)