2011 Fiscal Year Final Research Report
Molecular mechanisms of sexual plasticity during testicular differentiation and development in the medaka, Olyzias latipes
| Project/Area Number |
21570065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | University of Shizuoka |
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Principal Investigator |
KOBAYASHI Tohru 静岡県立大学, 環境科学研究所, 教授 (30221972)
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| Project Period (FY) |
2009 – 2011
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| Keywords | メダカ / 性的可塑性 / 性分化 / 精巣卵 / エストロゲン / dmrt1 / foxl2 |
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| Research Abstract |
This study focuses on the molecular mechanisms of sexual plasticity of testicular somatic cells during testis differentiation and development in the medaka, Olyzias latipes. In XY medaka of Hd-rR strain, estrogen treatment induces the female differentiation when estrogen expose to the fry before 30 dph(days post hatching). During XY sex reversal by estrogen, Sertoli cells showed the expression of foxl2, whereas the expression of male differentiation maker genes of germ-cell supporting cells were decreased and subsequently suppressed, such as gsdf and dmrt1 etc. Although estrogen induced testis-ova differentiation easily in mature fish, XY sex reversal(ovarian differentiation) was not induced. In mature XY fish treated by estrogen, foxl2 expression was induced. However, expression of male differentiation maker genes was not suppressed completely. These results suggest strongly that sexual plasticity of Sertoli cells is limited with the progression of testis differentiation in medaka.
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[Journal Article] Expression of 3υ-hydroxysteroid dehydrogenase(hsd3b), star and ad4bp/sf-1 during gonadal development in medaka(Oryzias latipes)2012
Author(s)
Nakamoto, M., Fukasawa, M., Tanaka, S., Shimamori, K., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata, N.
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Journal Title
Gen. Comp. Endocrinol
Volume: 176
Pages: 222-230
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[Journal Article] Comparative Responsiveness to Natural and Synthetic Estrogens of Fish Species Commonly Used in the Laboratory and Field Monitoring2011
Author(s)
Lange, A., Katsu, Y., Miyagawa, S., Ogino, Y., Urushutani, H., Kobayashi, T., Hirai, T., Shears, J. A., Nagae, M., Yamamoto, J., Ohnishi, Y., Oka, T., Tatarazako, N., Ohta, Y., Tyler, C. R. and Iguchi, T.
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Journal Title
Aquatic Toxicology
Volume: 109
Pages: 250-258
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[Journal Article] Gene expression profiles in the testis associated with testis-ova in adult Japanese medaka(Oryzias latipes) exposed to 17τ-ethinylestradiol2011
Author(s)
Hirakawa, I., Miyagawa, S., Katsu, Y., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Ogino, Y., Ohta, Y. and Iguchi, T.
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Journal Title
Chemosphere
Volume: 87
Pages: 668-674
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[Journal Article] The cloning and expression of medaka cholesterol side chain cleavage cytochrome P450(P450scc) during gonadal development2010
Author(s)
Nakamoto, M., Fukasawa, M., Orii, S., Shimamori, K., Maeda, T., Suzuki, A., Matsuda, M., Kobayashi, T., Nagahama, Y. and Shibata, N.
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Journal Title
Develop. Growth and Differ
Volume: 52
Pages: 385-395
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[Presentation] Comparative Responsiveness to test and sentinel fish species to natural and synthetic oestrogens2011
Author(s)
Lange, A., Katsu, Y., Miyagawa, S., Ogino, Y., Urushutani, H., Kobayashi, T., Hirai, T., Shears, J. A., Nagae, M., Yamamoto, 5 J., Ohnishi, Y., Oka, T., Tatarazako, N., Ohta, Y., Tyler, C. R. and Iguchi, T.
Organizer
9th International Symposium on Reproductive Physiology of Fish
Place of Presentation
India
Year and Date
20110000
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[Presentation] Histology and gene expression analysis in testis of medaka exposed by ethinylestradiol2010
Author(s)
Hirakawa, H., Kagami, Y., Tatarazako, N., Kobayashi, T., Kusano, T., Ogino, Y., Ohta, Y., Miyagawa, S., Iguchi, T.
Organizer
環境ホルモン学会第13回研究発表会
Place of Presentation
東京
Year and Date
20100000
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