2011 Fiscal Year Final Research Report

Elucidation of Activation Mechanism of Extracellular Matrix Protein in Related to Allergosis

Research Project

Project/Area Number	21570123
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Structural biochemistry
Research Institution	Japan Atomic Energy Agency
Principal Investigator	TAMADA Taro 独立行政法人日本原子力研究開発機構, 量子ビーム応用研究部門, 研究主幹 (50391248)
Co-Investigator(Kenkyū-buntansha)	ADACHI Motoyasu 独立行政法人日本原子力研究開発機構, 子ビーム応用研究部門, 研究副主幹 (60293958)
Co-Investigator(Renkei-kenkyūsha)	IZUHARA Kenji 佐賀大学, 医学部, 教授 (00270463)
Project Period (FY)	2009 – 2011
Keywords	ペリオスチン / アレルギー疾患 / 活性化機構
Research Abstract	Human periostin is an extracellular matrix protein in related to allergosis. We succeeded in preparation of functional region(R1-R4), including four sequential fasciclin 1 like domains, of human periostin using E. coli expression system combined with refolding method. Continuously, two anti-human periostin antibodies with sufficient binding ability to antigen were selected by gel filtration analysis, and then crystallization experiments using complex between R1-R4 and Fabs from these antibodies were carried out.

(1 results)