2011 Fiscal Year Final Research Report
Analysis of the role of electrostatic interactions between cytoplasmic domains of sarcoplasmic reticulum calcium pump
| Project/Area Number |
21570127
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Asahikawa Medical College |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 生体エネルギー転換 / カルシウムポンプ |
|---|
| Research Abstract |
I explored the roles of electrostatic interactions in the movement of cytoplasmic domain of sarcoplasmic reticulum calcium pump by using a kinetic analysis of site-directed mutants. In this study, I found a linear relationship between logarithm of rate constant and square of the activity coefficient. This plot revealed that electrostatic interactions between domains act like a guide line of domain motion. This plot would provide a useful tool for quantification of electrostatic effects in the enzyme reactions.
|
