2011 Fiscal Year Final Research Report
Identification of peptide ligands for lipid receptors
| Project/Area Number |
21570128
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Gunma University |
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Principal Investigator |
TAKEDA Shigeki 群馬大学, 大学院・工学研究科, 教授 (80282854)
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| Project Period (FY) |
2009 – 2011
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| Keywords | Gタンパク質共役受容体 / TGR5 / GPCR-Gα融合タンパク質 / ランダムペプチドライブラリー |
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| Research Abstract |
A random hexapeptide library was screened to identify novel peptide ligands for a TGR5, which is a G-protein-coupled receptor for bioactive steroids. We successfully identified a weak agonist peptide (Ac-WLFLHV-NH2 ) with EC50 of 630 nM, and a weak antagonist peptide (Ac-WVFLIV-NH2) with IC50 of 15 nM.
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Research Products
(20 results)
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[Journal Article] Genomic and proteomic chaacterization of the large Myoviridae bacteriophage TMA of the extreme thermophile2011
Author(s)
M. Tamakoshi, A. Murakami, M. Sugisawa, K. Tsuneizumi, S. Takeda, T. Saheki, T. Izumi, T. Akiba, K. Mitsuoka, H. Toh, A. Yamashita, F. Arisaka, M. Hattori, T. Oshima, A. Yamagishi
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Journal Title
Thermus thermophilus, Bacteriophage
Volume: 67
Pages: 837-841
Peer Reviewed
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[Journal Article] Observation of the membrane binding activity and domain structure of gpV, which comprises the tail spike of bacteriophage P22009
Author(s)
Y. Kageyama, M. Murayama, T. Onodera, S. Yamada, H. Fukada, M. Kudou, K. Tsumoto, Y. Toyama, S. Kado, K. Kubota, S. Takeda
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Journal Title
Biochemistry
Volume: 48
Pages: 10129-10135
Peer Reviewed
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