Regulatory mechanism of glycosylation

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Keio University (2010-2011); Mitsubishi Kagaku Institute of Life Sciences (2009)
• Principal Investigator: GOTO Satoshi 慶應義塾大学, 医学部, 特任講師 (60280575)
• Project Period (FY): 2009 – 2011
• Keywords: 糖鎖 / mRNA / ショウジョウバエ

Research Abstract

Glycosylation plays an important role in various physiological processes such as development, immunity and neural functions. However, mechanisms regulating glycosylation still remain largely unknown. To elucidate it, we performed a large scale screen using Drosophila RNAi library and identified novel genes. We analyzed the functions of the genes and found novel mechanisms of mRNA of glycosylation related genes.

Research Products

• [Journal Article] Identification of proteasome components required for apical localization of Chaoptin using functional genomics (2012)
  • Author(s): Yano, H., Yamamoto-Hino, M., Awano, W., Aoki-Kinoshita, K. F., Tsuda-Sakurai, K., Okano, H. and Goto, S.
  • Journal Title: J. Neurogenet Volume: vol.26 Pages: 53-63
  • DOI: DOI:10.3109/01677063.2012.661497
  • Peer Reviewed
• [Journal Article] Multistage MS/MS information obtained by deconvolution of energy-resolved mass spectra acquired by triple-quadrupole mass spectrometry (2011)
  • Author(s): Kanie, O., Kanie, Y., Daikoku, S., Shioiri, Y., Kurimoto, A., Mutsuga, S., Goto, S., Ito, Y. and Suzuki, K.
  • Journal Title: Rapid Commun. Mass Spectrom Volume: vol.25 Pages: 1617-1624
  • DOI: DOI:10.1002/rcm.5031
  • Peer Reviewed
• [Journal Article] Identification of genes required for neural-specific glycosylation using functional genomics (2010)
  • Author(s): Yamamoto-Hino, M., Kanie, Y., Awano, W., Aoki-Kinoshita, K. F., Yano, H., Nishihara, S., Okano, H., Ueda, R., Kanie, O. and Goto, S.
  • Journal Title: PLoS Genetics Volume: vol.6 Pages: e1001254
  • DOI: DOI:10.1371/journal.pgen.1001254
  • Peer Reviewed
• [Journal Article] Spatial and temporal regulation of glycosylation during Drosophila eye development (2009)
  • Author(s): Yano, H., Yamamoto-Hino, M. and Goto, S.
  • Journal Title: Cell. Tissue Res. Volume: vol.336 Pages: 137-147
  • DOI: DOI:10.1007/s00441-009-0753-
  • Peer Reviewed
• [Journal Article] Insight into the regulation of glycan synthesis in Drosophila chaoptin based on mass spectrometry (2009)
  • Author(s): Kanie, Y., Yamamoto-Hino, M., Karino, Y., Yokozawa, H., Nishihara, S., Ueda, R., Goto, S. and Kanie, O.
  • Journal Title: PLoS ONE Volume: vol.4 Pages: e5434
  • DOI: DOI:10.1371/journal.pone.0005434
  • Peer Reviewed
• [Presentation] Novel Roles of Glycosylation in Drosophila Innate Immunity (2011)
  • Author(s): 山本(日野)美紀、芝野孝子、粟野若枝、村岡正敏、岡野栄之、後藤聡
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜パシフィコ(横浜)
  • Year and Date: 20111213-16
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀、岡野栄之、後藤聡
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉)
  • Year and Date: 20111127-29
• [Presentation] 糖鎖関連遺伝子の大規模スクリーニング (2011)
  • Author(s): 山本(日野)美紀、蟹江善美、粟野若枝、木下聖子、西原祥子、岡野栄之、上田龍、蟹江治、後藤聡
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 20110921-24
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀、粟野若枝、芝野孝子、岡野栄之、後藤聡
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館(京都)
  • Year and Date: 20110921-24
• [Presentation] 自然免疫を制御する糖鎖修飾関連分子Senjuの解析 (2011)
  • Author(s): 山本(日野)美紀、芝野孝子、岡野栄之、後藤聡
  • Organizer: 第30回日本糖質学会年会
  • Place of Presentation: ハイブ長岡(新潟)
  • Year and Date: 2011-07-12
• [Book] NY, Structure, Function and Formation of Glycans in Drosophila, Glycans : Biochemistry, Characterization and Applications. (2012)
  • Author(s): Yamamoto-Hino, M., Okano, H., Kanie, O. and Goto, S.
  • Total Pages: 165-188
  • Publisher: Nova Science publishers, Inc.