2011 Fiscal Year Final Research Report
Development of Analytical System of Structure and Function of Membrane Proteins with Surface-enhanced Infrared Spectroscopy
| Project/Area Number |
21570165
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 膜タンパク質 / 赤外分光 / 構造・機能解析 / 可溶化 / 界面活性剤 / リン脂質 |
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| Research Abstract |
Experimental techniques on membrane proteins for solubilizing with short-chain phospholipids, reconstituting into partially fluorinated phospholipid vesicles and on a sensitive infrared detection with surface-enhanced effects have been developed for constructing an analytical system of structure and function of membrane proteins. It has been demonstrated that short-chain phospholipids are powerful for solubilization of membrane proteins and that vesicles of partially fluorinated phospholipids are suitable materials for incorporating membrane proteins under native conditions. An analytical system with surface-enhanced infrared spectroscopy has been developed for membrane proteins research.
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[Presentation] 新規部分フッ素化リン脂質1,2-Di(14,14,14,13,13,12,12,11,11-nonafluorotetradecanoil)-glycero-3-phosphorylcholine二重膜の熱物性と膜の安定性2011
Author(s)
上治瑛, 吉野賢, 高橋浩, 高木俊之, 馬場照彦, 金森敏幸, 園山正史
Organizer
第5回バイオ関連化学シンポジウム
Place of Presentation
つくば国際会議場(茨城県)
Year and Date
2011-09-13
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[Presentation] 日本生物物理学会第48回年会2010
Author(s)
M. Yoshino, H. Takahashi, T. Takagi,T. Baba, T. Kanamori, M. Sonoyama
Organizer
Structure and thermal properties of binary lipid bilayers of partially fluorinated and normal phosphatidylcholine
Place of Presentation
東北大学川内北キャンパス(宮城県)
Year and Date
2010-09-22
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